Recombinant Human CD44v5 Fc Chimera Avi-tag Protein, CF

Catalog #: AVI10146 Datasheet

Biotinylated

Catalog #	Availability	Size / Price	Qty
AVI10146-050

2 Images

All CD44 Proteins and Enzymes

Product Details

FAQs

Supplemental Products

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CD44v5 Fc Chimera Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When 2.00 μg/mL of Biotinylated Recombinant Human CD44v5 Fc Chimera Avi-tag protein is immobilized onto Streptavidin coated plate (Catalog # CP004) Recombinant Human LSECtin/CLEC4G (Catalog # 2947-CL) binds with an ED₅₀ of 10.0-60.0 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human CD44 protein

Human CD44v5 (Gln21-Thr222) NVDRNGTTAYEGNWNPEAHPPLIHHEHHEEEETPHSTST (Arg223-Trp269) Accession # P16070-12	IEGRMD	Human IgG₁ (Pro100-Lys330	Avi-tag
N-terminus			C-terminus

Accession #

NP_001001391.1

N-terminal Sequence
Analysis

Gln21 inferred from enzymatic pyroglutamate treatment revealing Ile22.

Structure / Form

Disulfide-linked homodimer

Biotinylated via Avi-tag

Predicted Molecular Mass

60 kDa

SDS-PAGE

100-120 kDa, under reducing conditions.

Product Datasheets

AVI10146

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI10146

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

View Larger

When 2.00 μg/mL of Biotinylated Recombinant Human CD44v5 Fc Chimera Avi-tag Protein (Catalog # AVI10146) is immobilized onto Streptavidin coated plate (CP004), Recombinant Human LSECtin/CLEC4G (2947-CL) binds with an ED₅₀ of 10.0‑60.0 ng/mL.

SDS-PAGE

View Larger

2 μg/lane of Biotinylated Recombinant Human CD44 Fc Chimera Avi-tag Protein (Catalog # AVI10146) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 100-120 kDa and 200‑240 kDa, respectively.

Background: CD44

CD44, also known as extracellular matrix receptor III (ECMRIII), is a non-kinase transmembrane glycoprotein that regulates numerous functions including cell proliferation, adhesion, migration, hematopoiesis, and lymphocyte activation. Mature human CD44 consists of an extracellular domain (ECD) with a hyaluronan‑binding, disulfide-stabilized link region and a stem region, a transmembrane domain, and a cytoplasmic domain. The full-length ECD of human CD44 shares 63% amino acid sequence identity with mouse CD44. In humans, CD44 is encoded by 19 exons with 10 constant in all isoforms. The standard form of CD44 (CD44s) is encoded by the 10 constant exons and is the shortest and most abundantly expressed isoform. The remaining 9, variant exons of CD44 can undergo complex alternative splicing and are expressed in a cell-specific manner and in the pathophysiology of many diseases. The N-terminal ECD of CD44 contains a binding site for Hyaluronan (HA), the most common ligand of CD44. CD44 exhibits multiple different functions and is aberrantly up-regulated among diverse tumors. It can bind HA and function as a "platform" for growth factors and metalloproteinases. CD44 can function as a co-receptor that modifies activity of receptors including MET and the ERBB family of tyrosine kinases. Further, CD44 can link the plasma membrane to the actin cytoskeleton via the ERM. CD44 has been found to bind E- and L-selectin on tumor cells and E-selectin on neutrophils. Additionally, distinct variants of CD44 have been found to interact with LSECtin and potentially mediate its function in the immune system. Human CD44v5 contains exon 10 and is associated with tumor progression and metastasis in many cancer types and is considered a functional cancer biomarker. Our Avi-tag Biotinylated CD44 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

Ponta, H. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:33.
Senbanjo, L.T. and Chellaiah, M.A. (2017) Front Cell Dev Biol. 5:18.
Chen, C. at al. (2018) J Hematol Oncol 11:64.
Xu, H. et al. (2020) Hematol Oncol 9:36.
Nagano, O. and H. Saya (2004) Cancer Sci. 95:930.
Hanley, W.D. et al. (2005) Cancer Res. 65:5812.
Katayama, Y. et al. (2005) J. Exp. Med. 18:1183.
Tang, L. et al. (2010) Eur. J. Immunol. 40:1185.

Entrez Gene IDs

960 (Human); 12505 (Mouse); 25406 (Rat); 100126860 (Porcine)

Alternate Names

CD44 antigen; CD44 molecule (Indian blood group); CD44; CD44R; CDw44; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; CSPG8; ECMR-III; epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; HCAM; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; Hyaluronate receptor; IN; LHR; MC56; MDU2; MDU2CD44 antigen (homing function and Indian blood group system); MDU3; MDU3CDW44; MIC4; MIC4MGC10468; MUTCH-I; Pgp1; PGP-1; PGP-I; Phagocytic glycoprotein 1; Phagocytic glycoprotein I