Home / CCL5/RANTES / Recombinant Human CCL5/RANTES (Mucin Stalk Chimera) Protein

Recombinant Human CCL5/RANTES (Mucin Stalk Chimera) Protein

Catalog #: 978-RN Datasheet

Carrier Free

Catalog # Availability Size / Price Qty
978-RN-025/CF

With Carrier

Catalog # Availability Size / Price Qty
978-RN-025
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CCL5/RANTES (Mucin Stalk Chimera) Protein Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR5. The ED50 for this effect is 0.1‑0.5 µg/mL.
Source
Mouse myeloma cell line, NS0-derived human CCL5/RANTES protein
Human RANTES
(Ser24 - Ser91)
Accession # P13501		 GS Human Fractalkine Mucin-like Stalk
(Phe103 - Gln341)
Accession # P78423		 KQN 6-His tag
N-terminus C-terminus
Accession #
P13501 (CCL5) & P78423 (CX3CL1 Mucin-like Stalk)
N-terminal Sequence
Analysis
Ser24
Predicted Molecular Mass
34 kDa
SDS-PAGE
85-105 kDa, reducing conditions

Product Datasheets

You must select a language.

x

978-RN (with carrier)

Product Datasheet

You must select a language.

x

978-RN/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

978-RN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

978-RN/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL5/RANTES

Fractalkine (also known as neurotactin and CX3CL1) is one of two known transmembrane chemokines and is the only member of the CX3C chemokine subfamily. Fractalkine is a type I membrane protein containing a chemokine domain tethered on a long mucin-like stalk that is connected to a transmembrane domain and a C-terminal intracellular domain. Expression of Fractalkine has been detected on endothelial cells, neurons, dendritic cells and a subset of mononuclear phagocytes. The expression of Fractalkine is upregulated in response to inflammatory signals. Fractalkine binds with high affinity to CX3CR1 and mediates the tight binding of leukocytes expressing CX3CR1 independently of G protein activation under flow conditions. In the RANTES/mucin-like stalk chimera, the chemokine domain of human Fractalkine is replaced by human RANTES (CCL5), a ligand for CCR1, CCR3 and CCR5.

References
  1. Haskell, C.A. et al. (2000) J. Biol. Chem. 275:34183.
Entrez Gene IDs
6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)
Alternate Names
CCL5; chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed, and presumably secreted; EoCP; Eosinophil chemotactic cytokine; RANTES; SISd; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human CCL5/RANTES (Mucin Stalk Chimera) Protein

There are currently no reviews for this product. Be the first to review Recombinant Human CCL5/RANTES (Mucin Stalk Chimera) Protein and earn rewards!

Have you used Recombinant Human CCL5/RANTES (Mucin Stalk Chimera) Protein?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review