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Recombinant Human CCL21/6Ckine (Mucin Stalk Chimera) Protein

Catalog #: 966-6C Datasheet

Carrier Free

Catalog # Availability Size / Price Qty
966-6C-025/CF

With Carrier

Catalog # Availability Size / Price Qty
966-6C-025
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Recombinant Human CCL21/6Ckine (Mucin Stalk Chimera) Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract 5-10 day cultured human peripheral blood lymphocytes (PBL). The ED50 for this effect is 0.5‑1.5 µg/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR7. The ED50 for this effect is 25-100 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human CCL21/6Ckine protein
Human 6Ckine
(Ser24 - Pro134)
(Pro41 Ser)
Accession # NP_002980		 GS Human Fractalkine mucin-like stalk
(Phe103 - Gln341)
Accession # P78423		 KQN 6-His tag
N-terminus C-terminus
Accession #
NP_002980 (6Ckine) & P78423 (Fractalkine Mucin-lik
N-terminal Sequence
Analysis
Ser24
Predicted Molecular Mass
38.3 kDa
SDS-PAGE
95-105 kDa, reducing conditions

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966-6C (with carrier)

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966-6C/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

966-6C

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

966-6C/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL21/6Ckine

Fractalkine (also known as neurotactin and CX3CL1) is one of two known transmembrane chemokines and is the only member of the CX3C chemokine subfamily. Fractalkine is a type I membrane protein containing a chemokine domain tethered on a long mucin-like stalk that is connected to a transmembrane domain and a C-terminal intracellular domain. Expression of Fractalkine has been detected on endothelial cells, neurons, dendritic cells and a subset of mononuclear phagocytes. The expression of Fractalkine is upregulated in response to inflammatory signals. Fractalkine binds with high affinity to CX3CR1 and mediates the tight binding of leukocytes expressing CX3CR1 independently of G protein activation under flow conditions. In the 6Ckine/mucin-like stalk chimera, the chemokine domain of human Fractalkine is replaced by human 6Ckine (CCL21), a ligand for CCR7. On a molar basis, soluble 6Ckine and 6Ckine/mucin-like stalk chimera are essentially equipotent in their ability to chemoattract cultured human lymphocytes.

References
  1. Haskell, C.A. et al. (2000) J. Biol. Chem. 275:34183.
Entrez Gene IDs
6366 (Human); 18829 (Mouse); 298006 (Rat)
Alternate Names
6Ckine; 6CkineSmall-inducible cytokine A21; Beta-chemokine exodus-2; CCL21; chemokine (C-C motif) ligand 21; CKb9; exodus-2; member 21; SCYA21; SCYA21MGC34555; secondary lymphoid tissue chemokine; SLC; SLCSecondary lymphoid-tissue chemokine; TCA-4

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