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Recombinant Human CCL1/I-309 Protein

Catalog #: 272-I
10 Citations 2 Reviews Datasheet

Carrier Free

Catalog # Availability Size / Price Qty
272-I-010/CF
272-I-050/CF

With Carrier

Catalog # Availability Size / Price Qty
272-I-010
272-I-050
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (10)
FAQs
Reviews (2)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CCL1/I-309 Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BW5147 mouse T lymphoma cells. The ED50 for this effect is 3-9 ng/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR8. The ED50 for this effect is 1.5-7.5 ng/mL.
Source
E. coli-derived human CCL1/I-309/TCA-3 protein
Lys24-Lys96
Accession #
P22362.1
N-terminal Sequence
Analysis
Lys24
Predicted Molecular Mass
8.5 kDa

Product Datasheets

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272-I (with carrier)

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272-I/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

272-I

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

272-I/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL1/I-309/TCA-3

Human CCL1 was initially identified by subtractive hybridization as a transcript that was present in a gamma /δ T cell line but not in EBV-transformed B cells. Human CCL1 has been assumed to be a homologue of the mouse TCA3. While the two proteins share only approximately 42% amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines. Human CCL1 and mouse TCA3 also share significant sequence homology in the 5’ flanking region of their genes.

CCL1 cDNA encodes a 96 amino acid residue precursor protein with a hydrophobic signal peptide that is cleaved to yield a 73 amino acid residue mature protein. The CCL1 sequence contains one potential N-linked glycosylation site and natural CCL1 secreted by activated T cells is a glycoprotein doublet of 15 - 16 kDa. The amino acid sequence of CCL1 identified the protein as a member of the chemokine beta subfamily.

References
  1. Miller, M.D. and M.S. Krangel (1992) Proc. Natl. Acad. Sci USA 89:2950.
  2. Miller, M.D. et al. (1990) J. Immunol. 145:2737.
Entrez Gene IDs
6346 (Human); 20290 (Mouse)
Alternate Names
C-C motif chemokine 1; CCL1; chemokine (C-C motif) ligand 1; I-309; inflammatory cytokine I-309; P500; SCYA1; SCYA1Small-inducible cytokine A1; SCYA2; SISe; small inducible cytokine A1 (I-309, homologous to mouse Tca-3); T lymphocyte-secreted protein I-309; TCA3; TCA-3

Citations for Recombinant Human CCL1/I-309 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Structural basis of antibody inhibition and chemokine activation of the human CC chemokine receptor 8
    Authors: Sun, D;Sun, Y;Janezic, E;Zhou, T;Johnson, M;Azumaya, C;Noreng, S;Chiu, C;Seki, A;Arenzana, TL;Nicoludis, JM;Shi, Y;Wang, B;Ho, H;Joshi, P;Tam, C;Payandeh, J;Comps-Agrar, L;Wang, J;Rutz, S;Koerber, JT;Masureel, M;
    Nature communications
    Species: Hamster, Human
    Sample Types: Transfected Whole Cells
    Applications: Bioassay
  2. CC chemokine CCL1 receptor CCR8 mediates conversion of mesenchymal stem cells to embryoid bodies expressing FOXP3+CCR8+ regulatory T cells
    Authors: NS Haque, A Tuteja, N Haque
    PLoS ONE, 2019-07-17;14(7):e0218944.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  3. CCR8(+)FOXp3(+) Treg cells as master drivers of immune regulation
    Authors: Y Barsheshet, G Wildbaum, E Levy, A Vitenshtei, C Akinseye, J Griggs, SA Lira, N Karin
    Proc. Natl. Acad. Sci. U.S.A., 2017-05-22;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012-07-26;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Mouse CCL8, a CCR8 agonist, promotes atopic dermatitis by recruiting IL-5+ T(H)2 cells.
    Authors: Islam SA, Chang DS, Colvin RA
    Nat. Immunol., 2011-01-09;12(2):167-77.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. CC chemokine ligand 1 is released into the airways of atopic asthmatics.
    Authors: Montes-Vizuet R, Vega-Miranda A, Valencia-Maqueda E, Negrete-Garcia MC, Velasquez JR, Teran LM
    Eur. Respir. J., 2006-03-15;28(1):59-67.
    Species: Human
    Sample Types: BALF, Cell Culture Supernates, N/A
    Applications: Bioassay, ELISA (Standard)
  7. Genetic fusions with viral chemokines target delivery of nonimmunogenic antigen to trigger antitumor immunity independent of chemotaxis.
    Authors: Ruffini PA, Biragyn A, Coscia M, Harvey LK, Cha SC, Bogen B, Kwak LW
    J. Leukoc. Biol., 2004-04-09;76(1):77-85.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Expression of functional CCR and CXCR chemokine receptors in podocytes.
    Authors: Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstadt H
    J. Immunol., 2002-06-15;168(12):6244-52.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Stromal-derived factor 1 inhibits the cycling of very primitive human hematopoietic cells in vitro and in NOD/SCID mice.
    Authors: Cashman J, Clark-Lewis I, Eaves A, Eaves C
    Blood, 2002-02-01;99(3):792-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. A cytokine-to-chemokine axis between T lymphocytes and keratinocytes can favor Th1 cell accumulation in chronic inflammatory skin diseases.
    Authors: Albanesi C, Scarponi C, Sebastiani S, Cavani A, Federici M, Sozzani S, Girolomoni G
    J. Leukoc. Biol., 2001-10-01;70(4):617-23.
    Applications: ELISA (Standard)

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Reviews for Recombinant Human CCL1/I-309 Protein

Average Rating: 4.5 (Based on 2 Reviews)

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Recombinant Human CCL1/I-309 Protein
By Anonymous on 11/04/2019
Application: Characterization prior to downstream assays.
Reason for Rating: SEC elution consistent with expected MW, material appears to be good quality. Resolution may be improved with additional material and method development
Other Recombinant Human CCL1/I-309 Protein 272-I
Recombinant Human CCL1/I-309 Protein
By Anonymous on 11/22/2017
Application: Immunoassay Standard
Immunoassay Standard Recombinant Human CCL1/I-309 Protein 272-I