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Recombinant Human AG-2/AGR2 His-tag Protein, CF

Catalog #: 10326-AG Datasheet
Catalog # Availability Size / Price Qty
10326-AG-050
Recombinant Human AG-2/AGR2 His-tag Protein Bioactivity
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human AG-2/AGR2 His-tag Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity

Measured by the ability of the immobilized protein to support the adhesion of PC-3 human prostate cancer cells. 

The ED50 for this effect is 0.3-1.8 µg/mL.

Source
E. coli-derived human AG-2/AGR2 protein
Arg21-Leu175, with a C-terminal 6-His tag
Accession #
NP_006399.1
N-terminal Sequence
Analysis
Met-Arg21
Structure / Form
Noncovalently-linked homodimer
Predicted Molecular Mass
19 kDa
SDS-PAGE
17-20 kDa, under reducing conditions

Product Datasheets

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10326-AG

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10326-AG

Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl, and TCEP.
Reconstitution Reconstitute at 500 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human AG-2/AGR2 His-tag Protein Bioactivity View Larger

Recombinant Human AG-2/AGR2 His-tag (Catalog # 10326-AG) supports the adhesion of PC-3 human prostate cancer cells. The ED50 for this effect is 0.03-0.18 μg/mL.

SDS-PAGE Recombinant Human AG-2/AGR2 His-tag Protein SDS-PAGE View Larger

2 μg/lane of Recombinant Human AG-2 His-tag (Catalog # 10326-AG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 17-20 kDa.

Background: AG-2/AGR2

AG-2 (Anterior gradient protein 2; also AGR2, GOB4 and HPC8) is an 18‑21 kDa member of the Protein disulfide-isomerase (PDI) family of enzymes. PDIs participate in the formation of transient disulfide linkages with molecules which aid in protein folding. Mature human AG-2 is 155 amino acids (aa) in length (aa 21‑175) and has a dimeric thioredoxin fold structure (1) which contains one cysteine (Cys81). It is expressed strongly in secretory cell types, such as prostate epithelium, small intestine goblet, trachea and lung, and is also expressed in estrogen receptor-positive breast cancer cell lines (2). Although AG-2 possesses a signal sequence, it appears to be rarely secreted, instead being found in the ER due the presence of a KTEL ER retention signal at its C-terminus (3). Over aa 21‑175, human AG-2 shares 94% aa identity with mouse AG-2. Over expressed in colorectal cancer stem cells, AG-2 is a marker regulated by the canonical Wnt/ beta -catenin pathway, AG-2 levels combined with elevated levels of beta -catenin are shown to indicate lower survival prognosis (4). Further, Wnt11-mediated signaling pathway has been implicated in secreted AG-2's role to promote metastasis of colorectal cancer (5). In prostate cancer, marker RAD9A affects the availability of AG-2. When added to RAD9A-depleted PC-3 cells, addition of AG-2 restores cell migration and adhesion properties (6). Additionally, AG-2 has been linked to pituitary adenomas as a biomarker (7).

References
  1. Patel, P. et al. (2013) ‎J. Mol. Biol. 425:929.
  2. Thompson, D. A. and Weigel, R. J. (1998) Biochem. Biophys. Res. Commun. 251:111.
  3. Gupta, A. et al. (2012) J. Biol. Chem. 287:4773.
  4. Lamichane, B. et al. (2019) Biochem. Biophys. Res. Commun. 515:600.
  5. Tian, S. et al. (2018) Experimental Cell Research. 364:198.
  6. Broustas, C. G. et al. (2018) Carcinogenesis. 40:164.
  7. Tohti, M. et al. (2017) Clinical Neurology and Neurosurgery. 154:19.
Long Name
Anterior Gradient 2 Homolog
Entrez Gene IDs
10551 (Human); 23795 (Mouse); 298961 (Rat)
Alternate Names
AG2; AG-2; AG2member 17; AGR2; anterior gradient 2 homolog (Xenopus laevis); anterior gradient homolog 2 (Xenopus laevis); GOB-4; hAG-2; HPC8; PDIA17; secreted cement gland homolog

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