Recombinant HCoV-OC43 Spike His-tag Protein, CF Summary
Product Specifications
Val15-Pro1297 (Arg754Ser, Arg757Ser), with a C-terminal 6-His tag
Analysis
Product Datasheets
10989-CV
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
10989-CV
| Formulation | Supplied as a 0.2 μm filtered solution in PBS. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
View Larger
2 μg/lane of Recombinant HCoV-OC43 Spike His-tag Protein (Catalog # 10989-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at >150 kDa.
Background: Spike
HCoV-OC43, a virus first isolated in 1960's that accounts for ~ 20% of the common cold, belongs to a family of viruses known as coronaviruses that are commonly comprised of a large plus-strand RNA genome and four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1, 2). Other well-known human coronaviruses include three viruses that cause relatively mild respiratory disease: HCoV-229E, HCoV-HKU1 and HCov-NL63, plus three viruses that cause the Severe Acute Respiratory Syndrome (SARS-CoV), the Middle East Respirator Syndrome (MERS-CoV), and the global pandemic Covid-19 (SARS-CoV2). While the S, E and M proteins build up the viral envelop, the N protein is involved transcription, replication, and packaging of the viral RNA genome into a helical ribonucleocapsid (RNP) (3, 4). The CoV-OC43 N protein is a ~50 kDa protein composed of two independent structural domains connected by a linker region. Both the N-terminal and the linker regions contain RNA binding domains, while the C-terminal region is responsible for the oligomerization of the N protein (5). The CoV‑OC43 N protein shares 64% amino acid sequence identity with CoV-HKU1 N protein. the N protein is an abundant protein during coronavirus infection and displays high immunogenic activity. Cross activity of antibodies among different strains should be rigorously tested when designing serological diagnostic kits (6, 7).
- St-Jean, J.R. et al. (2004) J. Virol. 78:8824.
- Vabret, A. et al. (2003) Fr Clin Infect Dis. 36:985.
- Chang, C.K. et al. (2006) J. Biomed. Sci. 13:59.
- Hurst, K.R. et al. (2009) J. Virol. 83:7221.
- Huang, C.Y. et al. (2009) Protein Sci. 18:2209.
- Chan, K.H. et al. (2005) Clin Diagn Lab Immunol. 12:1317.
- Mourez, T. et al. (2007) J. Virol Methods. 139:175.
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant HCoV-OC43 Spike His-tag Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant HCoV-OC43 Spike His-tag Protein, CF and earn rewards!
Have you used Recombinant HCoV-OC43 Spike His-tag Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
