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Recombinant Feline CD8 alpha Protein, CF

Catalog #: 9649-CD Datasheet
Catalog # Availability Size / Price Qty
9649-CD-050
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Summary Product Datasheets Carrier Free Reconstitution Calculator Background Related Research Areas

Recombinant Feline CD8 alpha Protein, CF Summary

Product Specifications

Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Bioassay data are not available.
Source
Mouse myeloma cell line, NS0-derived feline CD8 protein
Ala22-Tyr188, with a C-terminal 6-His tag
Accession #
P41688
N-terminal Sequence
Analysis
Ala22
Predicted Molecular Mass
19 kDa
SDS-PAGE
28-31 kDa, reducing conditions

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9649-CD

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9649-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD8

CD8 is a dimeric complex made up of two Ig superfamily members (1). The CD8 alpha chain is a type I transmembrane glycoprotein that can dimerize with either itself forming CD8 alpha alpha, or with a 30-35 kDa CD8 beta chain, forming CD8 alpha beta (2). CD8 alpha contains one V-type Ig-like domain in its extracellular region that binds to class I MHC molecules. CD8 alpha beta is a TCR co-receptor, while CD8 alpha alpha promotes T cell survival and differentiation (1, 3). The mature feline CD alpha consists of a 167 amino acid (aa) extracellular domain (ECD), and runs at ~30 kDa on reduced SDS-PAGE. Within the ECD, feline CD8 alpha shares 58%, 47%, 51%, and 69% amino acid sequence identity with human, mouse, porcine, and canine CD8 alpha, respectively.

References
  1. Laugel, B. et al. (2011) J. Leukoc. Biol. 90:1089.
  2. Snow, P.M. and C. Terhorst (1983) J. Biol. Chem. 258:14675.
  3. Cole, D.K. et al. (2012) Immunology 137:139.
Entrez Gene IDs
925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)
Alternate Names
CD_antigen: CD8a; CD8 antigen, alpha polypeptide (p32); CD8; CD8a molecule; CD8A; Leu2 T-lymphocyte antigen; LEU2; MAL; OKT8 T-cell antigen; p32; T cell co-receptor; T8 T-cell antigen; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2

FAQs

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