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Recombinant Equine IL-4 Protein

Catalog #: 1809-EL
3 Citations Datasheet

Carrier Free

Catalog # Availability Size / Price Qty
1809-EL-020/CF

With Carrier

Catalog # Availability Size / Price Qty
1809-EL-020
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (3)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Equine IL-4 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 10-60 ng/mL.
Source
E. coli-derived equine IL-4 protein
Lys26-Cys137
Accession #
NP_001075988
N-terminal Sequence
Analysis
Lys26
Predicted Molecular Mass
12.6 kDa

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1809-EL (with carrier)

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1809-EL/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1809-EL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1809-EL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-4

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa ‑ 18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1-3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Equine IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature equine IL-4 shares 53% - 60% aa sequence identity with bovine, goat, human, ovine, and porcine
IL-4 and 38%-40% aa sequence identity with mouse and rat IL-4. IL-4 exerts its effects through two receptor complexes (5, 6). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and
-21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2‑biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgE in B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (7-10). IL-4 plays a dominant role in the development of allergic inflammation and asthma (9, 11).

References
  1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
  2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
  3. Vandergrifft, E.V. et al. (1994) Vet. Immunol. Immunopathol. 40:379.
  4. Redfield, C. et al. (1991) Biochemistry 30:11029.
  5. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
  6. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
  7. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
  8. Corthay, A. (2006) Scand. J. Immunol. 64:93.
  9. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
  10. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
  11. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.
Long Name
Interleukin 4
Entrez Gene IDs
3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)
Alternate Names
B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; IL4E12; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra

Citations for Recombinant Equine IL-4 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Equine Arteritis Virus in Monocytic Cells Suppresses Differentiation and Function of Dendritic Cells
    Authors: NA Moyo, D Westcott, R Simmonds, F Steinbach
    Viruses, 2023-01-16;15(1):.
    Species: Equine
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Effects of priming with cytokines on intracellular survival and replication of Rhodococcus equi in equine macrophages
    Authors: LJ Berghaus, S Giguère, AI Bordin, ND Cohen
    Cytokine, 2017-12-12;102(0):7-11.
    Species: Equine
    Sample Types: Whole Cells
    Applications: Bioassay
  3. IL-4 stimulates the expression of CXCL-8, E-selectin, VEGF, and inducible nitric oxide synthase mRNA by equine pulmonary artery endothelial cells.
    Authors: Huang H, Lavoie-Lamoureux A, Moran K, Lavoie JP
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007-05-01;292(5):L1147-54.
    Species: Equine
    Sample Types: Whole Cells
    Applications: Bioassay

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