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Recombinant Equine FGF basic/FGF2/bFGF Protein

Catalog #: 8577-FB
1 Citation Datasheet

Carrier Free

Catalog # Availability Size / Price Qty
8577-FB-025/CF

With Carrier

Catalog # Availability Size / Price Qty
8577-FB-025
R&D Systems Recombinant Proteins and Enzymes
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Citations (1)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Equine FGF basic/FGF2/bFGF Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.2-1 ng/mL.
Source
E. coli-derived equine FGF basic/FGF2/bFGF protein
Pro10-Ser155
Accession #
E0AEV7
N-terminal Sequence
Analysis
Pro10 & Ala11
Predicted Molecular Mass
16 kDa
SDS-PAGE
18 kDa, reducing conditions

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8577-FB (with carrier)

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8577-FB/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8577-FB

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, TCEP and EDTA with BSA as a carrier protein.
Reconstitution Reconstitute at 250 μg/mL in water containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

8577-FB/CF

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, TCEP and EDTA.
Reconstitution Reconstitute at 250 μg/mL in water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF basic/FGF2/bFGF

FGF basic, also known as FGF-2 or HBGF-2, is a widely expressed member of the fibroblast growth factor family (1-3). FGF basic functions in angiogenesis, wound healing, tissue repair, learning and memory, and the morphogenesis of heart, bone, and brain (2, 4-6). It is up-regulated in response to inflammatory stimuli such as TNF-alpha, IL-1 beta, IL-2, PDGF, and nitric oxide (2). The up-regulation of FGF basic in many human tumors is associated with tumor vascularity (2, 3). Among FGF family members, only FGF acidic and FGF basic lack signal peptides and are secreted by an alternative pathway. FGF basic is additionally localized in the nucleus where it regulates gene expression (2, 7). In humans, the use of alternative start sites can produce 21-23 kDa isoforms found only in the nucleus, and these isoforms target the expression of distinct genes (7). FGF family proteins share 35-60% amino acid (aa) identity. The 18 kDa equine FGF basic shares 97-99% amino acid  identity with bovine, canine, human, mouse, and rat FGF basic (8). FGF basic binds with picomolar affinity to FGF R1c and 2c (1, 2). Its bioactivity is modulated by a number of other binding partners including heparin, Integrin alpha v beta 3, soluble FGF R1, FGF-BP, free gangliosides, Thrombospondin, Pentraxin 3/TSG-14, Fibrinogen,
alpha 2-Macroglobulin, PDGF, and CXCL4/PF4 (2). These molecules act as cellular coreceptors or adhesion partners, extracellular matrix decoys or reservoirs, and soluble scavengers or chaperones (2). In particular, the interaction of FGF basic with cell surface heparan sulfate proteoglycans (HSPG) is required for the binding and activation of FGF receptors (1, 2).

References
  1. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
  2. Presta, M. et al. (2005) Cytokine Growth Factor Rev. 16:159.
  3. Grose, R. and C. Dickson (2005) Cytokine Growth Factor Rev. 16:179.
  4. Rosenblatt-Velin, N. et al. (2005) J. Clin. Invest. 115:1724.
  5. Reuss, B. et al. (2003) Cell Tissue Res. 313:139.
  6. Su, N. et al. (2008) Front. Biosci. 13:2842.
  7. Claus, P. et al. (2003) J. Biol. Chem. 278:479.
  8. Abraham, J.A. et al. (1986) Science 233:545.
Long Name
Fibroblast Growth Factor basic
Entrez Gene IDs
2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)
Alternate Names
basic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF basic; FGF2; FGF-2; FGFBprostatropin; fibroblast growth factor 2 (basic); HBGF-2; heparin-binding growth factor 2; Prostatropin

Citation for Recombinant Equine FGF basic/FGF2/bFGF Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Peripheral blood-derived mesenchymal stem cells demonstrate immunomodulatory potential for therapeutic use in horses
    Authors: ALF Longhini, TE Salazar, C Vieira, T Trinh, Y Duan, LM Pay, S Li Calzi, M Losh, NA Johnston, H Xie, M Kim, RJ Hunt, MC Yoder, D Santoro, TM McCarrel, MB Grant
    PLoS ONE, 2019-03-14;14(3):e0212642.
    Species: Equine
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. What receptors does FGF basic bind?
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