Recombinant Cynomolgus Monkey Mer Fc Chimera Protein, CF Summary
Product Specifications
| Cynomolgus Monkey Mer (Ala23-Ala501) Accession # XP_005575320.1 | IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
10576-MR
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
10576-MR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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2 μg/lane of Recombinant Cynomolgus Monkey Mer Fc Chimera Protein (Catalog # 10576-MR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 118-140 kDa and 236-280 kDa, respectively.
Background: Mer
Tyrosine-protein Kinase Mer, also known as c-Mer and MerTK, is a member of the receptor tyrosine kinase subfamily TAM (Tyro3, Axl, and Mer). Mature cynomolgus Mer consists of 484 amino acid (aa) extracellular domain (ECD), a 20 aa transmembrane segment, and a 472 aa cytoplasmic domain. Within the ECD, cynomolgus Mer shares 95% and 99% sequence identity with human and rhesus monkey, respectively. Similar to Axl and Tyro3, the ECD of Mer contains two Ig-like motifs and two fibronectin type III motifs. Mer is not expressed in normal B- and T-cells but is expressed in neoplastic B- and T-cell lines (1-2). It also shows higher expression in immunosuppressive M2‑like macrophages (3). Mer is known to bind Gas6, Protein S, Tubby, Tubby-like protein 1 (Tulp1), and Galectin-3 (4-7). Binding of Gas6 lead to cell proliferation, migration or the prevention of apoptosis. Upon binding ligands via the Ig-like motif, Mer is dimerized to trans-autophosphorylate the kinase domain to induce downstream signaling. It has been shown that Mer signaling in macrophages induces M2 polarization, which promotes tumor growth, metastasis and evasion of anti-tumor immunity in tumor microenviroment (8). Inhibition of Mer, especially on leukocytes and macrophages, is an effective anti-cancer therapy (9).
- Graham, D.K. et al. (1994) Cell Growth Differ. 5:647.
- Graham, D.K. et al. (2006) Clin. Cancer Res. 12:2662.
- Shibata, T. et al. (2014) J. Immunol. 192:3569.
- Nagata, K. et al. (1996) J. Biol. Chem. 271:30022.
- Uehara, H. et al. (2008) J. Immunol. 180:2522.
- Caberoy, N.B. et al. (2010) EMBO J. 29:3898.
- Caberoy, N.B. et al. (2012) J. Cell Physiol. 227:401.
- Kim, S.Y. et al. (2016) Sci. Rep. 6:29673.
- Cummings, C.T. et al. (2013) Clin. Cancer Res. 19:5275.
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