Recombinant Cynomolgus Monkey ErbB2/Her2 Fc Protein, CF Summary
Product Specifications
| Cynomolgus Monkey ErbB2/Her2 (Thr23-Thr652) Accession # XP_005584091.2 | IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
10526-ER
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
10526-ER
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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2 μg/lane of Recombinant Cynomolgus Monkey ErbB2/Her2 Fc Protein (10526-ER) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 115-140 kDa and 230-280 kDa, respectively.
Background: ErbB2/Her2
ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I transmembrane glycoprotein member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factors. It is widely expressed in epithelial cells and has also been found to be over‑expressed in a large number of breast carcinomas. Among the ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other members of the ErbB family (ErbB1 (EGFR), ErbB3, ErbB4) to form higher affinity signaling complexes. Human ErbB2 consists of 1255 amino acids (aa) including a 22 aa signal sequence, a 630 aa extracellular domain (ECD), a 23 aa transmembrane region, and a 580 aa cytoplasmic domain. Within the ECD, cynomolgus ErbB2 shares and 99% aa sequence identity with human ErbB2. Phosphoinositide 3-kinase has been shown to play a role in ErbB2 signal transduction. The cytoplasmic domain of ErbB2 has been shown to associate with beta-catenin and plakoglobin. ErbB2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation (1-10).
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- Yamamoto, T. et al. (1986) Nature 319:230.
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- Codony-Servat, J. et al. (1999) Cancer Res. 59:1196.
- Carraway, K.L. 3rd et al. (1994) J. Biol. Chem. 269:14303.
- Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
- Schaefer, G. et al. (1999) J. Biol. Chem. 274:859.
- Schlessinger, J. (2000) Cell 103:211.
- Hellyer, N.J. et al. (2001) J. Biol. Chem. 276:42153.
- Daly, R.J. (1999) Growth Factors 16:255.
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