Recombinant Cynomolgus Monkey EGFR His-tag Protein, CF Summary
Product Specifications
Leu25-Ser645, with a C-terminal 6-His tag
Analysis
Product Datasheets
10405-ER
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
10405-ER
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 200 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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When Recombinant Cynomolgus Monkey EGFR His-tag (Catalog # 10405-ER) is immobilized 20 µg/mL, 100 µL/well, the concentration of Recombinant Human Pro-EGF (aa21-1023) Protein (Catalog # 4289-EG) that produces 50% of the optimal binding response is approximately 100-600 ng/mL.
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2 μg/lane of Recombinant Cynomolgus Monkey EGFR His-tag (Catalog # 10405-ER) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 95-109 kDa.
Background: EGFR
Epidermal growth factor receptor (EGFR) is a member of a subfamily of receptor tyrosine kinases comprised of four members: EGFR (also known as HER-1, ErbB1, or ErbB), ErbB2 (Neu, HER-2), ErbB3 (HER-3), and ErbB4 (HER-4). All family members are type I transmembrane glycoproteins with an extracellular domain (ECD) containing two cysteine-rich domains separated by a spacer region and a cytoplasmic domain containing a tyrosine kinase domain followed by multiple tyrosine autophosphorylation sites (1, 2). Several soluble isoforms lacking the intracellular domain are generated by alternate splicing (3, 4). The mature ECD of cynomolgus EGFR shares 99% and 90% amino acid sequence identity with human and mouse EGFR, respectively. EGFR binds a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, and epigen (1, 2). Ligand binding induces EGFR homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, heterodimerization tyrosine phosphorylation and cell signaling (5‑7). EGFR can also be recruited to form heterodimers with the ligand‑activated ErbB3 or ErbB4. EGFR signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis (5-7). EGFR is over-expressed in a wide variety of tumors and is the target of several anti-cancer drugs (8).
- Singh, A.B. and R.C. Harris (2005) Cell. Signal. 17:1183.
- Shilo, B.Z. (2005) Development 132:4017.
- Guillaudeau, A. et al. (2012) PLoS One. 7:1.
- Reiter J.L. et al. (2001) Genomics 71:1.
- Freed, D. M. et al. (2017) Cell. 171:683.
- Burgess, A.W. et al. (2003) Mol. Cell 12:541.
- Faria, J.A. et al. (2016) BBRC. 478:39.
- Lee, C.K. et al. (2017) J. Thoracic Oncology. 12:403.
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