Recombinant Cotton Rat sCD4 Protein, CF

Catalog #: 6676-CD Datasheet

Catalog #	Availability	Size / Price	Qty
6676-CD-050

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Recombinant Cotton Rat sCD4 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of NIH‑3T3 mouse embryonic fibroblast cells.

The ED₅₀ for this effect is 0.2-1.0 μg/mL after a 1 hour incubation at
37 °C.

Optimal dilutions should be determined by each laboratory for each application.

Source

Mouse myeloma cell line, NS0-derived cotton rat CD4 protein
Met1-Pro393, with a C-terminal 6-His tag

N-terminal Sequence
Analysis

Lys26

Predicted Molecular Mass

42.4 kDa

SDS-PAGE

50-52 kDa, reducing conditions

Product Datasheets

6676-CD

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6676-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD4

CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations (1). CD4 contains four immunoglobulin-like domains in its extracellular region. Within the ECD, human CD4 shares approximately 52% aa sequence identity with mouse and rat CD4, while mouse and rat CD4 share 73% identity. CD4 is expressed along with CD8 on double positive T cells during their development in the thymus. Either CD4 or CD8 expression is then lost, giving rise to single positive (SP) CD4⁺ or CD8⁺ mature T cells (2). CD4⁺ SP cells, also known as T helper cells, further differentiate into multiple subsets of CD4⁺ cells including Th1, Th2, Th17, Tfh, and Treg cells which regulate humoral and cellular immunity (3). CD4 is re‑expressed on circulating CD8⁺ T cells upon activation and contributes to their cytotoxic effector activity (4). In human, CD4 is additionally expressed on macrophages, neutrophils, monocytes, NK cells, and neurons and glial cells in the brain (5 ‑ 8). Similar CD4 distribution between species cannot be assumed as demonstrated by its presence on macrophages in human and rat but not in mouse (5). CD4 binds directly to MHC class II molecules on antigen presenting cells (9). This interaction contributes to the formation of the immunological synapse which is focused around the TCR-MHC class II-antigenic peptide interaction (1, 10). Palmitoylation of two cysteine residues in the cytoplasmic tail of CD4 promotes the localization of CD4 in lipid rafts and its ability to augment TCR signaling via activation of the tyrosine kinase Lck (11). CD4 also functions as a chemotactic receptor for IL-16 and, in human, as a coreceptor for the gp120 surface glycoprotein of HIV-1 (6, 12 ‑ 14). CD4 associates with adhesion molecule CD44 at the cell surface, and triggers CD44-mediated cell adhesion (15).

References

Vignali, D.A.A. (2010) J. Immunol. 184:5933.
Alarcon, B. and H.M. van Santen (2010) Sci. Signal. 3:pe11.
Wan, Y.Y. and R.A. Flavell (2009) Mol. Cell Biol. 1:20.
Kitchen, S.G. et al. (2005) Proc. Natl. Acad. Sci. 102:3794.
Crocker, P.R. et al. (1987) J. Exp. Med. 166:613.
Biswas, P. et al. (2003) Blood 101:4452.
Bernstein, H.B. et al. (2006) J. Immunol. 177:3669.
Funke, I. et al. (1987) J. Exp. Med. 165:1230.
Doyle, C. and J.L. Strominger (1987) Nature 330:256.
Huppa, J.B. et al. (2010) Nature 463:963.
Fragoso, R. et al. (2003) J. Immunol. 170:913.
Cruikshank, W.W. et al. (1994) Proc. Natl. Acad. Sci. 91:5109.
Klatzmann, D. et al. (1984) Nature 312:767.
Dagleish, A.G. et al. (1984) Nature 312:763.
Umberto, D. et al. (1999) International Immunology
11(7):1085.

Entrez Gene IDs

920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)

Alternate Names

CD_antigen: CD4; CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4