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Recombinant Canine TNF RI/TNFRSF1A Protein

Catalog #: 4017-TR Datasheet

Carrier Free

Catalog # Availability Size / Price Qty
4017-TR-025/CF

With Carrier

Catalog # Availability Size / Price Qty
4017-TR-025
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Recombinant Canine TNF RI/TNFRSF1A Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 0.04-0.2 µg/mL in the presence of 15 ng/mL of rcaTNF-alpha.
Source
E. coli-derived canine TNF RI/TNFRSF1A protein
Ile22-Thr211, with an N-terminal Met
Accession #
XP_854474
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
21.6 kDa

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4017-TR (with carrier)

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4017-TR/CF (carrier free)

Product Datasheet

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4017-TR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

4017-TR/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TNF RI/TNFRSF1A

TNF receptor 1 (TNF RI; also called TNF R-p55/p60, TNFRSF1A and CD120a) is a type I transmembrane protein that belongs to the TNF receptor superfamily (1, 2). TNF RI is widely expressed and is present on the cell surface as a trimer of 55 kDa subunits. It serves as a receptor for both TNF-alpha and TNF-beta /lymphotoxin. Each subunit contains four TNF-alpha trimer-binding cysteine-rich domains (CRD) in its extracellular domain (ECD) (1 - 6). TNF-alpha binding to TNF R1 induces the sequestration of TNF RI in lipid rafts, where it activates NF kappa B and is cleaved by ADAM-17/TACE (7, 8). Release of the 28 - 34 kDa TNF RI ECD occurs constitutively, and in response to products of pathogens such as LPS, CpG DNA or S. aureus protein A (1, 7 - 12). Full-length TNF RI may also be released in exosome-like vesicles (12). This release helps to resolve inflammatory reactions as it down-regulates cell surface TNF RI and provides soluble TNF RI to bind TNF-alpha (6, 13, 14). Exclusion from lipid rafts causes endocytosis of TNF RI complexes and induces apoptosis (7, 15). Although there is a second receptor for TNF-alpha (TNF R2), TNF RI is thought to mediate most of the cellular effects of TNF-alpha (3). TNF R1 is essential for proper development of lymph node germinal centers and Peyer’s patches, and for combating intracellular pathogens such as Listeria monocytogenes (1 - 3). Canine TNF RI is a 452 amino acid (aa) protein (16). Based on human, its ECD will contain a PLAD domain that mediates constitutive trimer formation (6). The PLAD domain will be followed by four CRDs, a transmembrane domain, and a cytoplasmic sequence that contains a neutral sphingomyelinase activation domain and a death domain. The ECD of canine TNF RI shows approximately 87%, 75%, 80%, 66% and 67% aa identity with feline, porcine, human, rat and mouse TNF RI, respectively.

References
  1. Pfeffer, K. (2003) Cytokine Growth Factor Rev. 14:185. 
  2. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1.  
  3. Peschon, J.J. et al. (1998) J. Immunol. 160:943. 
  4. Banner, D.W et al. (1993) Cell 73: 431. 
  5. Medvedev, A.E. et al. (1996) J. Biol. Chem. 271:9778. 
  6. Chan, F.K. et al. (2000) Science 288:2351. 
  7. Legler, D.F. et al. (2003) Immunity 18:655. 
  8. Tellier, E. et al. (2006) Exp. Cell Res. 312:3969.
  9. Xanthoulea, S. et al. (2004) J. Exp. Med. 200:367.
  10. Jin, L. et al. (2000) J. Immunol. 165:5153.
  11. Gomez, M.I. et al. (2006) J. Biol. Chem. 281:20190.
  12. Islam, A. et al. (2006) J. Biol. Chem. 281:6860.
  13. Garton, K.J. et al. (2006) J. Leukoc. Biol. 79:1105.
  14. McDermott, M.F. et al. (1999) Cell 97:133.
  15. Schneider-Brachert, W. et al. (2004) Immunity 21:415.
  16. GenBank Accession # XP_854474.
Long Name
Tumor Necrosis Factor Receptor I
Entrez Gene IDs
7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)
Alternate Names
CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor

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