Recombinant Canine FGF basic/FGF2/bFGF Protein Summary
Product Specifications
Pro10-Ser155 (Lys36Asn)
Analysis
Product Datasheets
8575-FB (with carrier)
8575-FB/CF (carrier free)
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8575-FB
| Formulation | Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, TCEP and EDTA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 250 μg/mL in water containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
8575-FB/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, TCEP and EDTA. |
| Reconstitution | Reconstitute at 250 μg/mL in water. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: FGF basic/FGF2/bFGF
FGF basic, also known as FGF-2 or HBGF-2, is a widely expressed member of the fibroblast growth factor family (1-3). FGF basic functions in angiogenesis, wound healing, tissue repair, learning and memory, and the morphogenesis of heart, bone, and brain (2, 4-6). It is up-regulated in response to inflammatory stimuli such as TNF-alpha, IL-1 beta, IL-2, PDGF, and nitric oxide (2). The up-regulation of FGF basic in many human tumors is associated with tumor vascularity (2, 3). Among FGF family members, only FGF acidic and FGF basic lack signal peptides and are secreted by an alternative pathway. FGF basic is additionally localized in the nucleus where it regulates gene expression (2, 7). In humans, the use of alternative start sites can produce 21-23 kDa isoforms found only in the nucleus, and these isoforms target the expression of distinct genes (7). FGF family proteins share 35-60% amino acid (aa) identity. The 18 kDa canine FGF basic shares 97-99% amino acid identity with bovine, equine, human, mouse, and rat FGF basic (8). FGF basic binds with picomolar affinity to FGF R1c and 2c (1, 2). Its bioactivity is modulated by a number of other binding partners including heparin, Integrin alpha v beta 3, soluble FGF R1, FGF-BP, free gangliosides, Thrombospondin, Pentraxin 3/TSG-14, Fibrinogen,
alpha 2-Macroglobulin, PDGF, and CXCL4/PF4 (2). These molecules act as cellular co-receptors or adhesion partners, extracellular matrix decoys or reservoirs, and soluble scavengers or chaperones (2). In particular, the interaction of FGF basic with cell surface heparan sulfate proteoglycans (HSPG) is required for the binding and activation of FGF receptors (1, 2).
- Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
- Presta, M. et al. (2005) Cytokine Growth Factor Rev. 16:159.
- Grose, R. and C. Dickson (2005) Cytokine Growth Factor Rev. 16:179.
- Rosenblatt-Velin, N. et al. (2005) J. Clin. Invest. 115:1724.
- Reuss, B. et al. (2003) Cell Tissue Res. 313:139.
- Su, N. et al. (2008) Front. Biosci. 13:2842.
- Claus, P. et al. (2003) J. Biol. Chem. 278:479.
- Abraham, J.A. et al. (1986) Science 233:545.
Citations for Recombinant Canine FGF basic/FGF2/bFGF Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Neural stem/progenitor cells from adult canine cervical spinal cord have the potential to differentiate into neural lineage cells
Authors: Kim, WK;Son, YS;Lim, JH;Kim, WH;Kang, BJ;
BMC veterinary research
Species: Canine
Sample Types: Whole Cells, Spheroid
Applications: Bioassay -
Omental implantation of BOECs in hemophilia dogs results in circulating FVIII antigen and a complex immune response.
Authors: Ozelo M, Vidal B, Brown C, Notley C, Hegadorn C, Webster S, Harpell L, Ahlin J, Winterborn A, Handforth J, Arruda V, Hough C, Lillicrap D
Blood, 2014-05-14;123(26):4045-53.
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay
FAQs
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What receptors does FGF basic bind?
FGF receptor specificity has been reviewed in multiple citations. Please find more information at: //www.rndsystems.com/resources/articles/fibroblast-growth-factors-and-their-receptors
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