Reagent Diluent Concentrate 2

Catalog #: DY995
41 Citations 28 Reviews Datasheet

10X, 5 x 21 mL

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DY995

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DY999B: Substrate Reagent Pack

DY999: Substrate Reagent Pack

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All Buffers, Chelators and Reagents ELISAs

Product Details

Citations (41)

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Reviews (28)

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Reagent Diluent Concentrate 2 Summary

INTENDED USE & DESCRIPTION

Reagent Diluent Concentrate 2 (10X) (Part # 841380) contains 5 vials (21 mL/vial) of a 10% BSA solution in a concentrated formula which can be diluted to make a working solution at a range of final BSA concentrations suitable for the assay being run.

STORAGE & STABILITY

Store unopened at 2-8 °C. Stable for up to 30 days after opening when stored at 2-8 °C. Make all solutions fresh or filter sterilize and store for up to 1 week at 2-8 °C.

REAGENT PREPARATION

For assays that require 1% BSA in PBS, dilute 1:10 in deionized water before use. For assays that require 0.1% BSA in TBS, dilute 1:100 with Tris buffer + 0.05% Tween® 20, pH 7.2-7.4.

TECHNICAL HINTS & LIMITATIONS OF THE PROCEDURE

The Reagent Diluent will require additional components for some DuoSets® ELISA Development Systems. Please refer to the product-specific package insert for more information.

Specifications

Source

N/A

Shipping Conditions

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Applications

ELISA

Product Datasheets

Product Datasheet

Citations for Reagent Diluent Concentrate 2

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

41 Citations: Showing 1 - 10
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Rapid Microfluidic Immuno-Biosensor Detection System for the Point-of-Care Determination of High-Sensitivity Urinary C-Reactive Protein
Authors: Chen, SJ;Lu, SY;Tseng, CC;Huang, KH;Chen, TL;Fu, LM;
Biosensors 2024-05-30
A new mRNA structure prediction based approach to identifying improved signal peptides for bone morphogenetic protein 2
Authors: Wilkinson, P;Jackson, B;Fermor, H;Davies, R;
BMC biotechnology 2024-05-23
Chronic Glaucoma Induced in Rats by a Single Injection of Fibronectin-Loaded PLGA Microspheres: IOP-Dependent and IOP-Independent Neurodegeneration
Authors: Munuera, I;Aragon-Navas, A;Villacampa, P;Gonzalez-Cela, MA;Subías, M;Pablo, LE;Garcia-Feijoo, J;Herrero-Vanrell, R;Garcia-Martin, E;Bravo-Osuna, I;Rodrigo, MJ;
International journal of molecular sciences 2023-12-19
Dynamic genetic adaptation of Bacteroides thetaiotaomicron during murine gut colonization
Authors: Kennedy, MS;Zhang, M;DeLeon, O;Bissell, J;Trigodet, F;Lolans, K;Temelkova, S;Carroll, KT;Fiebig, A;Deutschbauer, A;Sidebottom, AM;Lake, J;Henry, C;Rice, PA;Bergelson, J;Chang, EB;
Cell reports 2023-08-29
Effect of Expansion Media on Functional Characteristics of Bone Marrow-Derived Mesenchymal Stromal Cells
Authors: Jakl, V;Popp, T;Haupt, J;Port, M;Roesler, R;Wiese, S;Friemert, B;Rojewski, MT;Schrezenmeier, H;
Cells 2023-08-19
Tunable degrees of neurodegeneration in rats based on microsphere-induced models of chronic glaucoma
Authors: MJ Rodrigo, I Bravo-Osun, M Subias, A Montolío, J Cegoñino, T Martinez-R, S Mendez-Mar, A Aragón-Nav, D Garcia-Her, LE Pablo, R Herrero-Va, AP Del Paloma, E Garcia-Mar
Scientific Reports, 2022-11-30;12(1):20622. 2022-11-30
Determination of Blood NOTCH3 Extracellular Domain and Jagged-1 Levels in Healthy Subjects
Authors: H Kim, B Jang, YJ Kim, JC Choi
International Journal of Molecular Sciences, 2022-09-11;23(18):. 2022-09-11
Tumour DDR1 promotes collagen fibre alignment to instigate immune exclusion
Authors: X Sun, B Wu, HC Chiang, H Deng, X Zhang, W Xiong, J Liu, AM Rozeboom, BT Harris, E Blommaert, A Gomez, RE Garcia, Y Zhou, P Mitra, M Prevost, D Zhang, D Banik, C Isaacs, D Berry, C Lai, K Chaldekas, PS Latham, CA Brantner, A Popratilof, VX Jin, N Zhang, Y Hu, MA Pujana, TJ Curiel, Z An, R Li
Nature, 2021-11-03;0(0):. 2021-11-03
Topological isolation of developmental regulators in mammalian genomes
Authors: HJ Wu, A Landshamme, EK Stamenova, A Bolondi, H Kretzmer, A Meissner, F Michor
Nature Communications, 2021-08-12;12(1):4897. 2021-08-12
The NFIB-ERO1A axis promotes breast cancer metastatic colonization of disseminated tumour cells
Authors: F Zilli, P Marques Ra, P Auf der Ma, C Jehanno, A Sethi, MM Coissieux, T Eichlisber, L Sauteur, A Rouchon, L Bonapace, J Pinto Cout, R Rad, MR Jensen, A Banfi, MB Stadler, M Bentires-A
Embo Molecular Medicine, 2021-03-10;0(0):e13162. 2021-03-10

Detection of Plasmodium falciparum malaria in 1ï¿½h using a simplified enzyme-linked immunosorbent assay
Authors: E de la Sern, K Arias-Alpí, LN Borgheti-C, A Sanchez-Ca, E Sulleiro, F Zarzuela, P Bosch-Nico, F Salvador, I Molina, M Ramírez, X Fernàndez-, A Sánchez-Mo, E Baldrich
Analytica chimica acta, 2021-01-30;1152(0):338254. 2021-01-30

Stabilization of surface-bound antibodies for ELISA based on a reversable zeolitic imidazolate framework-8 coating
Authors: L Kang, S Smith, C Wang
Journal of colloid and interface science, 2020-12-24;588(0):101-109. 2020-12-24

Differentially expressed circular RNAs in a murine asthma model
Authors: H Bao, Q Zhou, Q Li, M Niu, S Chen, P Yang, Z Liu, L Xia
Molecular Medicine Reports, 2020-10-20;22(6):5412-5422. 2020-10-20

bFGF blockade reduces intraplaque angiogenesis and macrophage infiltration in atherosclerotic vein graft lesions in ApoE3*Leiden mice
Authors: L Parma, HAB Peters, TJ Sluiter, KH Simons, P Lazzari, MR de Vries, PHA Quax
Sci Rep, 2020-09-29;10(1):15968. 2020-09-29

Selective Increment of Synovial Soluble TYRO3 Correlates with Disease Severity and Joint Inflammation in Patients with Rheumatoid Arthritis
Authors: J Vullings, JP Vago, CEJ Waterborg, RM Thurlings, MI Koenders, PLEM van Lent, PM van der Kr, FA Amaral, FAJ van de Loo
J Immunol Res, 2020-09-11;2020(0):9690832. 2020-09-11

BMP9 signaling promotes the normalization of tumor blood vessels
Authors: C Viallard, C Audiger, N Popovic, N Akla, K Lanthier, I Legault-Na, H Melichar, S Costantino, S Lesage, B Larrivée
Oncogene, 2020-02-10;0(0):. 2020-02-10

Electrostatic complex of neurotrophin 4 with dendrimer nanoparticles: controlled release of protein in vitro and in vivo
Authors: M D?bkowska, D Rogi?ska, P K?os, A Sobu?, M Adamczak, Z Litwi?ska, A Machali?sk, B Machali?sk
Int J Nanomedicine, 2019-08-01;14(0):6117-6131. 2019-08-01

Circulating Lactonase Activity but Not Protein Level of PON-1 Predicts Adverse Outcomes in Subjects with Chronic Kidney Disease
Authors: CJ Mohammed, Y Xie, PS Brewster, S Ghosh, P Dube, T Sarsour, AL Kleinhenz, EL Crawford, D Malhotra, RW James, PA Kalra, ST Haller, DJ Kennedy
J Clin Med, 2019-07-15;8(7):. 2019-07-15

Intrapulmonary Autoantibodies to HSP72 Are Associated with Improved Outcomes in IPF
Authors: R Mills, A Mathur, LM Nicol, JJ Walker, AA Przybylski, AC Mackinnon, SEM Howie, WAH Wallace, I Dransfield, N Hirani
J Immunol Res, 2019-04-11;2019(0):1845128. 2019-04-11

Growth Factor-Mediated Tenogenic Induction of Multipotent Mesenchymal Stromal Cells Is Altered by the Microenvironment of Tendon Matrix
Authors: SP Roth, S Schubert, P Scheibe, C Gro beta, W Brehm, J Burk
Cell Transplant, 2018-09-25;0(0):9636897187922. 2018-09-25

Soluble interleukin-27 receptor alpha is a valuable prognostic biomarker for acute graft-versus-host disease after allogeneic haematopoietic stem cell transplantation
Authors: S Liu, J Han, H Gong, Y Li, X Bao, J Qi, H Liu, J Chen, X Wu, Y Xu, S Ma, D Wu
Sci Rep, 2018-07-09;8(1):10328. 2018-07-09

Serum Soluble Vascular Cell Adhesion Molecule-1 Overexpression Is a Disease Marker in Patients with First-Time Diagnosed Antinuclear Antibodies: A Prospective, Observational Pilot Study
Authors: M Oleszowsky, MF Seidel
Biomed Res Int, 2018-02-01;2018(0):8286067. 2018-02-01

Plant synthetic GP4 and GP5 proteins from porcine reproductive and respiratory syndrome virus elicit immune responses in pigs
Authors: CH An, S Nazki, SC Park, YJ Jeong, JH Lee, SJ Park, A Khatun, WI Kim, YI Park, JC Jeong, CY Kim
Planta, 2018-01-08;0(0):. 2018-01-08

IRF5 distinguishes severe asthma in humans and drives Th1 phenotype and airway hyperreactivity in mice
Authors: TB Oriss, M Raundhal, C Morse, RE Huff, S Das, R Hannum, MC Gauthier, KL Scholl, K Chakrabort, SM Nouraie, SE Wenzel, P Ray, A Ray
JCI Insight, 2017-05-18;2(10):. 2017-05-18

Prostate cancer downregulated SIRP-? modulates apoptosis and proliferation through p38-MAPK/NF-?B/COX-2 signaling
Authors: C Yao, G Li, M Cai, Y Qian, L Wang, L Xiao, F Thaiss, B Shi
Oncol Lett, 2017-04-21;13(6):4995-5001. 2017-04-21

Effect of selective small molecule inhibitors on MMP-9 and VEGFR-1 expression in p16-positive and -negative squamous cell carcinoma
Authors: B Kramer, JD Schultz, C Hock, A Sauter, BA Stuck, K Hörmann, R Birk, C Aderhold
Oncol Lett, 2017-03-13;13(5):3269-3276. 2017-03-13

Viral transduction of renin rapidly establishes persistent hypertension in diverse murine strains.
Authors: Harlan S, Ostroski R, Coskun T, Yantis L, Breyer M, Heuer J
Am J Physiol Regul Integr Comp Physiol, 2015-06-24;309(5):R467-74. 2015-06-24

Identification of longitudinally dynamic biomarkers in Alzheimer's disease cerebrospinal fluid by targeted proteomics.
Authors: Wildsmith K, Schauer S, Smith A, Arnott D, Zhu Y, Haznedar J, Kaur S, Mathews W, Honigberg L
Mol Neurodegener, 2014-06-06;9(0):22. 2014-06-06

Optofluidic laser for dual-mode sensitive biomolecular detection with a large dynamic range.
Authors: Wu X, Oo M, Reddy K, Chen Q, Sun Y, Fan X
Nat Commun, 2014-04-30;5(0):3779. 2014-04-30

Oral and subcutaneous therapy of canine atopic dermatitis with recombinant feline interferon omega.
Authors: Litzlbauer P, Weber K, Mueller R
Cytokine, 2014-01-14;66(1):54-9. 2014-01-14

A Monoclonal Antibody-GDNF Fusion Protein Is Not Neuroprotective and Is Associated with Proliferative Pancreatic Lesions in Parkinsonian Monkeys.
Authors: Ohshima-Hosoyama S, Simmons HA, Goecks N
PLoS ONE, 2012-06-20;7(6):e39036. 2012-06-20

Influence of galectin-9/Tim-3 interaction on herpes simplex virus-1 latency.
Authors: Reddy PB, Sehrawat S, Suryawanshi A
J. Immunol., 2011-10-21;187(11):5745-55. 2011-10-21

Combined Flt3L/TK gene therapy induces immunological surveillance which mediates an immune response against a surrogate brain tumor neoantigen.
Authors: King GD, Muhammad AK, Larocque D
Mol. Ther., 2011-04-19;19(10):1793-801. 2011-04-19

Nectin 4 overexpression in ovarian cancer tissues and serum: potential role as a serum biomarker.
Authors: Derycke MS, Pambuccian SE, Gilks CB
Am. J. Clin. Pathol., 2010-11-01;134(5):835-45. 2010-11-01

Normal ranges of angiogenesis regulatory proteins in human platelets.
Authors: Peterson JE, Zurakowski D, Italiano JE
Am. J. Hematol., 2010-07-01;85(7):487-93. 2010-07-01

Soluble erythropoietin receptor contributes to erythropoietin resistance in end-stage renal disease.
Authors: Khankin EV, Mutter WP, Tamez H
PLoS ONE, 2010-02-16;5(2):e9246. 2010-02-16

First-trimester follistatin-like-3 levels in pregnancies complicated by subsequent gestational diabetes mellitus.
Authors: Thadhani R, Powe CE, Tjoa ML
Diabetes Care, 2009-12-10;33(3):664-9. 2009-12-10

A novel and sensitive ELISA reveals that the soluble form of IL-13R-alpha2 is not expressed in plasma of healthy or asthmatic subjects.
Authors: O'Toole M, Legault H, Ramsey R, Wynn TA, Kasaian MT
Clin. Exp. Allergy, 2008-02-25;38(4):594-601. 2008-02-25

Nanobacteria are mineralo fetuin complexes.
Authors: Raoult D, Drancourt M, Azza S, Nappez C, Guieu R, Rolain JM, Fourquet P, Campagna B, La Scola B, Mege JL, Mansuelle P, Lechevalier E, Berland Y, Gorvel JP, Renesto P
PLoS Pathog., 2008-02-08;4(2):e41. 2008-02-08

Human tumor-derived exosomes selectively impair lymphocyte responses to interleukin-2.
Authors: Clayton A, Mitchell JP, Court J, Mason MD, Tabi Z
Cancer Res., 2007-08-01;67(15):7458-66. 2007-08-01

Interaction between interferon gamma and insulin-like growth factor-1 in hippocampus impacts on the ability of rats to sustain long-term potentiation.
Authors: Maher FO, Clarke RM, Kelly A, Nally RE, Lynch MA
J. Neurochem., 2006-02-08;96(6):1560-71. 2006-02-08