Rat VEGF₁₆₄ Alexa Fluor® 594-conjugated Antibody

Catalog #: FAB564T Datasheet

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FAB564T-100UG

All VEGF Antibodies

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Rat VEGF₁₆₄ Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat VEGF₁₆₄ in direct ELISAs and Western blots. Shows 50 - 100% cross-reactivity with recombinant mouse (rm) VEGF₁₂₀, rmVEGF₁₆₄, recombinant human (rh) VEGF₁₂₁, rhVEGF₁₆₅, and rhVEGF₁₈₉

Source

Monoclonal Mouse IgG2b Clone # 123704

Purification

Protein A or G purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat VEGF₁₆₄
Ala27-Arg190
Accession # AAA41211.1

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: VEGF

Vascular Endothelial Growth Factor (VEGF or VEGF-A), also known as Vascular Permeability Factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure. VEGF164 appears to be the most abundant and potent isoform, followed by VEGF120 and VEGF188. Rat VEGF164 is an approximately 50 kDa molecular weight homodimer sharing 97% aa sequence identity with corresponding regions of mouse, 88% with human and bovine, 90% with feline and equine, and 89% with canine and porcine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells. Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity. Human VEGF165 binds the Semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2. VEGF is required during embryogenesis and functions to regulate the proliferation, migration, and survival of endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle. Pathologically, it is involved in tumor angiogenesis and vascular leakage. Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus. VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, Oncostatin M (OSM) and TNF-alpha.

Long Name

Vascular Endothelial Growth Factor

Entrez Gene IDs

7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)

Alternate Names

MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor