Rat Total MMP-9 Quantikine ELISA Kit

Catalog #: RMP900
15 Citations 3 Reviews Datasheet

Catalog #	Availability	Size / Price	Qty
RMP900

2 Images

All MMP-9 ELISAs

Product Details

Procedure

Citations (15)

FAQs

Supplemental Products

Reviews (3)

Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Rat Total MMP-9 Quantikine ELISA Kit Summary

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), Platelet-poor Heparin Plasma (10 uL)

Sensitivity

0.028 ng/mL

Assay Range

0.2 - 10 ng/mL

Specificity

Natural and recombinant rat MMP-9 (Pro-, active, and TIMP-complexed)

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Rat Total MMP-9 Immunoassay is a 4.5 hour solid phase ELISA designed to measure total rat MMP-9 (Pro-, active, and TIMP-complexed MMP-9) in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant rat MMP-9 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant protein. Results obtained using natural rat MMP-9 showed dose-response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat MMP-9.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Platelet-poor Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.39	1.22	5.27	0.51	1.18	3.55
Standard Deviation	0.027	0.068	0.238	0.035	0.046	0.153
CV%	6.9	5.6	4.5	6.9	3.9	4.3

Recovery

The recovery of rat MMP-9 spiked into various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Samples (n=4)	94	87-107
Platelet-poor Heparin Plasma (n=4)	92	80-103
Serum (n=4)	97	90-115

Linearity

To assess the linearity of the assay, samples containing high concentrations of rat MMP-9 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data

N/A

View Larger

Rat Total MMP-9 ELISA Standard Curve

Product Datasheets

Product Datasheet

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MMP-9

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

Long Name:

Matrix Metalloproteinase 9

Entrez Gene IDs:

4318 (Human); 17395 (Mouse); 81687 (Rat); 102117693 (Cynomolgus Monkey)

Alternate Names:

92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 3.4.24.35; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase

Related Research Areas

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.

Prepare all reagents, standard dilutions, and samples as directed in the product insert.
Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

50 µL Assay Diluent

Add 50 µL of Assay Diluent to each well.

50 µL Standard, Control, or Sample

Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

100 µL Conjugate

Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
Aspirate and wash 5 times.

100 µL Substrate Solution

Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

100 µL Stop Solution

Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Rat Total MMP-9 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
Filter your results:

Filter by:

In Vivo Study on Doxycycline Protective Mechanisms during Myocardial Ischemia Injury in Rats
Authors: Krzywonos-Zawadzka, A;Olejnik, A;Sawicki, G;Bil-Lula, I;
Biomedicines
Species: Rat
Sample Types: Tissue Homogenates
Therapeutic role of mesenchymal stem cells and platelet-rich plasma on skin burn healing and rejuvenation: A focus on scar regulation, oxido-inflammatory stress and apoptotic mechanisms
Authors: Tammam, BMH;Habotta, OA;El-Khadragy, M;Abdel Moneim, AE;Abdalla, MS;
Heliyon
Species: Rat
Sample Types: Tissue Homogenates
Therapeutic Effect of Enzymatically Hydrolyzed Cervi Cornu Collagen NP-2007 and Potential for Application in Osteoarthritis Treatment
Authors: Kim, HR;Lee, SH;Noh, EM;Choi, B;Seo, HY;Jang, H;Kim, SY;Park, MH;
International journal of molecular sciences
Species: Rat
Sample Types: Cell Culture Supernates, Serum
Punica granatum L. Polyphenolic Extract as an Antioxidant to Prevent Kidney Injury in Metabolic Syndrome Rats
Authors: A Radajewska, J Szyller, J Niewiadoms, A Noszczyk-N, I Bil-Lula
Oxidative Medicine and Cellular Longevity, 2023-01-05;2023(0):6144967.
Species: Rat
Sample Types: Tissue Homogenates
A novel rat model of chronic subdural hematoma: Induction of inflammation and angiogenesis in the subdural space mimicking human-like features of progressively expanding hematoma
Authors: X Xu, D Wang, Z Han, B Wang, W Gao, Y Fan, F Li, Z Zhou, C Gao, J Xiong, S Zhou, S Zhang, G Yang, R Jiang, J Zhang
Brain research bulletin, 2021-04-28;172(0):108-119.
Species: Rat
Sample Types: Tissue Homogenates
Changes in Gene and Protein Expression of Metalloproteinase-2 and -9 and Their Inhibitors TIMP2 and TIMP3 in Different Parts of Fluoride-Exposed Rat Brain
Authors: A ?ukomska, I Baranowska, K Dec, A Pilutin, M Tarnowski, K Jakubczyk, W ?wiere??o, M Skórka-Maj, D Chlubek, I Gutowska
International Journal of Molecular Sciences, 2020-12-31;22(1):.
Species: Rat
Sample Types: Tissue Homogenates
The antihypertensive agent hydralazine reduced extracellular matrix synthesis and liver fibrosis in nonalcoholic steatohepatitis exacerbated by hypertension
Authors: Y Yuan, H Naito, K Kitamori, S Hashimoto, T Asano, T Nakajima
PLoS ONE, 2020-12-14;15(12):e0243846.
Species: Rat
Sample Types: Serum
Liposomal Curcumin is Better than Curcumin to Alleviate Complications in Experimental Diabetic Mellitus
Authors: AE Bulboac?, AS Porfire, LR Tefas, PM Boarescu, SD Bolboac?, IC St?nescu, AC Bulboac?, G Dogaru
Molecules, 2019-02-27;24(5):.
Species: Rat
Sample Types: Plasma
Cardioprotective effect of MMP-2-inhibitor-NO-donor hybrid against ischaemia/reperfusion injury
Authors: A Krzywonos-, A Franczak, A Olejnik, M Radomski, JF Gilmer, G Sawicki, M Wo?niak, I Bil-Lula
J. Cell. Mol. Med., 2019-02-07;0(0):.
Species: Rat
Sample Types: Tissue Homogenates
Matrix Metalloproteinase-Mediated Blood-Brain Barrier Dysfunction in Epilepsy
Authors: RG Rempe, AMS Hartz, ELB Soldner, BS Sokola, SR Alluri, EL Abner, RJ Kryscio, A Pekcec, J Schlichtig, B Bauer
J. Neurosci., 2018-04-09;0(0):.
Species: Rat
Sample Types: Tissue Homogenates

2-Methoxyestradiol Causes MMP 9 Mediated Transactivation of EGFR and Angiotensin Type 1 Receptor Downregulation in Rat Aortic Smooth Muscle Cells
Authors: B Ogola, Y Zhang, L Iyer, T Thekkumkar
Am. J. Physiol., Cell Physiol., 2018-01-24;0(0):.
Species: Rat
Sample Types: Cell Culture Supernates

Implication of Free Fatty Acids in Thrombin Generation and Fibrinolysis in Vascular Inflammation in Zucker Rats and Evolution with Aging
Authors: J Lagrange, M Didelot, A Mohamadi, LA Walton, S Bloemen, B de Laat, H Louis, SN Thornton, B Derby, MJ Sherratt, B Fève, P Challande, R Akhtar, JK Cruickshan, P Lacolley, V Regnault
Front Physiol, 2017-11-22;8(0):949.
Species: Rat
Sample Types: Plasma

Ferulaldehyde Improves the Effect of Methotrexate in Experimental Arthritis
Authors: L Slovák, K Švík, D Mihalová, J Tóth, S Czigle, ? Pašková, F Bilka, K Bauerová
Molecules, 2017-11-06;22(11):.
Species: Rat
Sample Types: Plasma

Aliskiren has chondroprotective efficacy in a rat model of osteoarthritis through suppression of the local renin-angiotensin system
Authors: K Yan, Y Shen
Mol Med Rep, 2017-07-28;0(0):.
Species: Rat
Sample Types: Serum

Resveratrol suppresses neuroinflammation in the experimental paradigm of autism spectrum disorders
Authors: Ranjana Bhandari
Neurochem. Int, 2016-12-23;103(0):8-23.
Species: Rat
Sample Types: Serum