Rat MAG/Siglec-4a Antibody Summary
Gly20-Pro516
Accession # P07722
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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MAG/Siglec‑4a in Rat Brain. MAG/Siglec‑4a was detected in perfusion fixed frozen sections of rat brain (cerebellum) using 25 µg/mL Goat Anti-Rat MAG/Siglec‑4a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF538) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to processes of oligodendrocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MAG/Siglec-4a
MAG (Myelin-Associated Glycoprotein), a type I transmembrane glycoprotein containing five Ig-like domains in its extracellular domain is an adhesion molecule belonging to the immunoglobin superfamily. Within this superfamily, MAG, CD22, CD33, Schwann cell myelin protein, and sialoadhesin which bind specifically to cell-surface glycan containing sialic acid residues define the I-type sialyl lectin subgroup, also called the sialoadhesin family. Sialoadhesins mediate diverse biological processes through recognition of specific sialyted glycans on cell surface. MAG is expressed on myelinating oligodenrocytes and Schwann cells, and preferentially recognizes alpha 2, 3-linked sialic acid on O-linked glycans and gangliosides. MAG exists as two isoforms which differ in the sequence and length of the cytoplasmic tail. The large form (71 kDa) and small form (67 kDa) arise from alternative spliced mRNAs. Although MAG might encounter haematopoietic cells and lymphocytes under pathologic conditions, it would normally be expected to interact with neuronal cells. It has been shown that MAG promotes axonal growth from neonatal DRG neurons and embryonic spinal neurons, but is a potent inhibitor of axonal re-growth from adult DRG and postnatal cerebellar neurons. MAG plays an important role in the interaction between axons and myelin. A soluble form of MAG containing the extracellular domain is released from myelin in large quantities and identified in normal human tissues and in tissues from patients with neurological disorders. This soluble MAG might contribute to the lack of CNS neuron regeneration after injury.
- Kelm, S. et al. (1994) Current Biology 4:965.
- McKerracher, L. et al. (1994) Neuron 13:805.
- Tang, S. et al. (1997) Molecular and Cellular Neuroscience 9:333.
- Cai, D. et al. (1999) Neuron 22:89.
Product Datasheets
Citations for Rat MAG/Siglec-4a Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Efficacy of Nerve-Derived Hydrogels to Promote Axon Regeneration Is Influenced by the Method of Tissue Decellularization
Authors: VK Kuna, A Lundgren, LO Anerillas, P Kelk, M Brohlin, M Wiberg, PJ Kingham, LN Novikova, G Andersson, LN Novikov
International Journal of Molecular Sciences, 2022-08-06;23(15):.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Nonneuronal control of the differential distribution of myelin along retinal ganglion cell axons in the mouse.
Authors: Yang, Xiaoli, Zou, Haidong, Jung, Gila, Richard, Gisbert, Linke, Stephan, Ader, Marius, Bartsch, Udo
Invest Ophthalmol Vis Sci, 2013-12-02;54(13):7819-27.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
In vitro expanded stem cells from the developing retina fail to generate photoreceptors but differentiate into myelinating oligodendrocytes.
Authors: Czekaj M, Haas J, Gebhardt M, Muller-Reichert T, Humphries P, Farrar J, Bartsch U, Ader M
PLoS ONE, 2012-07-25;7(7):e41798.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Netrin-1 is a novel myelin-associated inhibitor to axon growth.
Authors: Löw K, Culbertson M, Bradke F, Tessier-Lavigne M, Tuszynski MH
J. Neurosci., 2008-01-30;28(5):1099-108.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Cleavage of myelin associated glycoprotein by matrix metalloproteinases.
Authors: Milward E, Kim KJ, Szklarczyk A, Nguyen T, Melli G, Nayak M, Deshpande D, Fitzsimmons C, Hoke A, Kerr D, Griffin JW, Calabresi PA, Conant K
J. Neuroimmunol., 2007-12-11;193(1):140-8.
Species: Rat
Sample Types: Recombinant Protein
Applications: Western Blot -
Neuregulin 1-erbB signaling is necessary for normal myelination and sensory function.
Authors: Chen S, Velardez MO, Warot X, Yu ZX, Miller SJ, Cros D, Corfas G
J. Neurosci., 2006-03-22;26(12):3079-86.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
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