Rat IL-5 Antibody

Catalog #: MAB5451 Datasheet

Catalog #	Availability	Size / Price	Qty
MAB5451
MAB5451-SP

Cell Proliferation Induced by IL‑5 and Neutralization by Rat IL‑5 Antibody.

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Rat IL-5 Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat IL-5 in direct ELISAs. In direct ELISAs, 50% cross-reactivity with mouse IL-5 is observed and no cross‑reactivity with human, equine, canine, feline, or rhesus monkey IL-5 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 299257

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant rat IL-5
Met20-Val132
Accession # Q08125

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Neutralization

Measured by its ability to neutralize IL‑5-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 0.02-0.08 µg/mL in the presence of 1.25 ng/mL Recombinant Rat IL‑5.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by IL‑5 and Neutralization by Rat IL‑5 Antibody. Recombinant Rat IL-5 (Catalog # 545-RL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-5 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat IL-5 Monoclonal Antibody (Catalog # MAB5451). The ND₅₀ is typically 0.02-0.08 µg/mL.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-5

Interleukin-5 (IL-5) is a secreted glycoprotein that belongs to the alpha -helical group of cytokines (1 ‑ 3). Unlike other family members, it is present as a covalently linked antiparallel dimer (4, 5). The cDNA for rat IL-5 encodes a signal peptide and a 113 amino acid (aa) secreted mature protein. Mature rat IL-5 shares 70%, 94%, 56%, 63%, 59% and 59%, aa sequence identity with human, mouse, canine, equine, feline and porcine IL-5, respectively. IL-5 is primarily produced by CD4+ Th2 cells, but also by activated eosinophils, mast cells, EBV-transformed B cells, Reed-Sternberg cells in Hodgkin’s disease, and IL-2-stimulated invariant natural killer T cells (iNKT) (1 ‑ 3, 6 ‑ 8). IL-5 increases production and mobilization of eosinophils and CD34+ progenitors from the bone marrow and causes maturation of eosinophil precursors outside the bone marrow (1, 6, 9, 10). The receptor for human IL-5, mainly expressed by eosinophils, but also found on basophils and mast cells, consists of a unique ligand-binding subunit (IL-5 R alpha ) and a shared signal‑transducing subunit, beta c (3, 6, 11). IL-5 R alpha first binds IL-5 at low affinity, then associates with preformed beta c dimers, forming a high-affinity receptor (12). IL-5 also binds proteoglycans, potentially enhancing its activity (13). Soluble forms of IL-5 R alpha antagonize IL-5 and can be found in vivo (10, 14). In humans, IL-5 primarily affects cells of the eosinophilic lineage, and promotes their differentiation, maturation, activation, migration and survival, while in mice IL-5 also enhances Ig class switching and release from B1 cells (1 ‑ 3, 9, 10, 15, 16). IL-5 also promotes differentiation of basophils and primes them for histamine and leukotriene release (17).

References

Rosenberg, H. F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.
Elsas, P.X. and M. I. G. Elsas (2007) Curr. Med. Chem. 14:1925.
Martinez-Moczygemba, M. and D. P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
Minamitake, Y. et al. (1990) J. Biochem. 107:292.
McKenzie, A. N. et al. (1991) Mol. Immunol. 28:155.
Shakoory, B. et al. (2004) J. Interferon Cytokine Res. 24:271.
Lalani, T. et al. (1999) Ann. Allergy Asthma Immunol. 82:317.
Sakuishi, K. et al. (2007) J. Immunol. 179:3452.
Clutterbuck, E. J. et al. (1989) Blood 73:1504.
Cameron, L. et al. (2000) J. Immunol. 164:1538.
Tavernier, J. et al. (1991) Cell 66:1175.
Zaks-Zilberman, M. et al. (2008) J. Biol. Chem. 283:13398.
Lipscombe, R. et al. (1998) J. Leukocyte Biol. 63:342.
Tavernier, J. et al. (2000) Blood 95:1600.
Kopf, M. et al. (1996) Immunity 4:15.
Horikawa, K. and K. Takatsu (2006) Immunology 118:497.
Denburg, J. A. et al. (1991) Blood 77:1462.

Long Name

Interleukin 5

Entrez Gene IDs

3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)

Alternate Names

BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I