Rat IL-17/IL-17A Antibody

Catalog #: MAB8410 Datasheet

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB8410-100
MAB8410-SP

All IL-17/IL-17A Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat IL-17/IL-17A Antibody Summary

Species Reactivity

Rat

Specificity

Detects Rat IL‑17/IL‑17A in direct and capture ELISAs. In direct Elisa, no cross-reactivity with Rat IL17F was observed.

Source

Recombinant Monoclonal Mouse IgG_2B Clone # 905521R

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant rat IL-17A + rat IL-17F
(Met1-Ser150,I46L) and (Met1-161)
Accession # Q61453

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with rat anti-human IL‑17/IL‑17A monoclonal antibody (Catalog # MAB84101).This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17/IL-17A

Rat IL-17A/F is an approximately 37 kDa, secreted, disulfide-linked glycosylated heterodimeric protein comprised of two members of the IL-17 family of cytokines, IL-17A and IL-17F (1, 2). Members of this family demonstrate a structural motif termed a cysteine knot that also characterizes a large superfamily of growth factors such as TGF-beta (1). Although most cysteine knot superfamily members use three intrachain disulfide bonds to create a knot, IL-17 family molecules generate the same structural form with only two disulfide links (1, 3-5). Mature rat IL-17A and IL-17F share 60% and 59% amino acid sequence identity with human and 61% and 90% with mouse IL-17A and IL-17F, respectively. They also share 50% aa sequence identity with each other (1). IL-17A/F heterodimer and the IL-17A and IL-17F homodimers are produced by IL-23 activated Th17 cells (1, 6-10, 14). The widely expressed receptors IL-17 RA and IL-17 RC form a heterodimer for the binding of
IL-17A and IL-17F, as well as the heterodimeric IL-17A/F (6, 11, 12). IL-17A/F is a biologically active protein that induces chemokine production and airway neutrophilia with intermediate potency between IL-17A (most potent) and IL-17F (least potent) (7, 12). It is up-regulated in immune cells during inflammatory arthritis and contributes to disease severity (13).

References

Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265.
Kolls, J.K. and A. Linden (2004) Immunity 21:467.
Gu, C. et al. (2013) Cytokine 64:477.
Cheung, P.F.Y. et al. (2008) J. Immunol. 180:5625.
Liang, S.C. et al. (2007) J. Immunol. 179:7791.
Ouyang, W. et al. (2008) Immunity 28:454.
Steinman, L. (2007) Nat. Med. 13:139.
Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.
Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
Wright, J.F. et al. (2008) J. Immunol. 181:2799.
Sarkar, S. et al. (2014) Clin. Exp. Immunol. 177:652.
Spolski, R. et al. (2009) Eur. J. Immunol. 3: 658.

Long Name

Interleukin 17

Entrez Gene IDs

3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)

Alternate Names

CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A