Rat IL-13 Antibody

Catalog #: MAB1945 Datasheet

Catalog #	Availability	Size / Price	Qty
MAB1945
MAB1945-SP

Cell Proliferation Induced by IL‑13 and Neutralization by Rat IL‑13 Antibody.

1 Image

All IL-13 Antibodies

Product Details

FAQs

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat IL-13 Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat IL-13 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse (rm) IL‑4, recombinant human IL-13, rmIL-13, or recombinant rhesus macaque IL‑13 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 296202

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant rat IL-13
Thr19-His131
Accession # P42203

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Rat IL‑13 (Catalog # 1945-RL)
under non-reducing conditions only

Neutralization

Measured by its ability to neutralize IL‑13-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 1-4 µg/mL in the presence of 15 ng/mL Recombinant Rat IL‑13.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

View Larger

Cell Proliferation Induced by IL‑13 and Neutralization by Rat IL‑13 Antibody. Recombinant Rat IL‑13 (Catalog # 1945-RL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL‑13 (15 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat IL‑13 Monoclonal Antibody (Catalog # MAB1945). The ND₅₀ is typically 1-4 µg/mL.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-13

IL-13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4⁺ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1-3). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration (4, 5). Mature rat IL-13 shares 59%, 75%, and 60% amino acid sequence identity with human, mouse, and rhesus IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (6, 7). IL-13 has diverse activities on numerous cell types (8). On macrophages, IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, up‑regulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 up‑regulates IL-6 while down‑regulating IL‑1 and TNF-alpha production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 R alpha 1, triggering IL-13 R alpha 1 association with IL-4 R alpha. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex (9, 10). Additionally, IL-13 binds with high affinity to IL-13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (11-14). IL-13 R alpha 2 regulates the bioavailability of both IL-13 and IL-4 and is over-expressed in glioma and several bronchial pathologies (10, 15, 16). Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13 R alpha 2 (17).

References