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Rat IgG2B Alexa Fluor® 647-conjugated Isotype Control

Catalog #: IC013R
2 Citations Datasheet
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IC013R
R&D Systems Antibodies
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Citations (2)
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Rat IgG2B Alexa Fluor® 647-conjugated Isotype Control Summary

Specificity
Selected for use as a rat IgG2B isotype control for flow cytometry applications.
Source
Monoclonal Rat IgG2B Clone # 141945
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 5 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2B Fc
Alternate Names
IgG2B

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Citations for Rat IgG2B Alexa Fluor® 647-conjugated Isotype Control

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. TGF-?-mediated silencing of genomic organizer SATB1 promotes Tfh cell differentiation and formation of intra-tumoral tertiary lymphoid structures
    Authors: Chaurio RA, Anadon CM, Lee Costich T et al.
    Immunity
  2. Spaceflight Activates Protein Kinase C Alpha Signaling and Modifies the Developmental Stage of Human Neonatal Cardiovascular Progenitor Cells
    Authors: J Baio, AF Martinez, LL Bailey, N Hasaniya, M Pecaut, M Kearns-Jon
    Stem Cells Dev., 2018-02-12;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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