Rat IgG2B Alexa Fluor® 594-conjugated Isotype Control

Catalog #: IC013T Datasheet

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IC013T

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Rat IgG2B Alexa Fluor® 594-conjugated Isotype Control Summary

Specificity

Selected for use as a rat IgG_2B isotype control for flow cytometry applications.

Source

Monoclonal Rat IgG_2B Clone # 141945

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

KLH

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Control

Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 5 μL/10⁶ cells.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G2B Fc

Alternate Names

IgG2B

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Product Datasheet

FAQs

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