Rat IgG1 APC-conjugated Isotype Control

Catalog # Availability Size / Price Qty
IC005A
R&D Systems Antibodies
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Product Details
Citations (4)
FAQs
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Reviews (1)

Rat IgG1 APC-conjugated Isotype Control Summary

Specificity
Selected for use as rat IgG1 isotype control for flow cytometry applications.
Source
Monoclonal Rat IgG1 Clone # 43414
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG1

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G1
Alternate Names
IgG1; Igh-4; RGD1359539; VH7183

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Citations for Rat IgG1 APC-conjugated Isotype Control

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Selective Uptake of Carboxylated Multi-Walled Carbon Nanotubes by Class A Type 1 Scavenger Receptors and Impaired Phagocytosis in Alveolar Macrophages
    Authors: Ruhung Wang, Rishabh Lohray, Erik Chow, Pratima Gangupantula, Loren Smith, Rockford Draper
    Nanomaterials (Basel)
  2. Circulating blood extracellular vesicles as a tool to assess endothelial injury and chemotherapy toxicity in adjuvant cancer patients
    Authors: G Bar-Sela, I Cohen, A Avisar, D Loven, A Aharon
    PLoS ONE, 2020-10-27;15(10):e0240994.
    Species: Human
    Sample Types: Plasma
    Applications: Isotype Control
  3. Comparative Transcriptomic Analysis Identifies a Range of Immunologically Related Functional Elaborations of Lymph Node Associated Lymphatic and Blood Endothelial Cells
    Authors: Stella J. Berendam, Alexander F. Koeppel, Nicole R. Godfrey, Sherin J. Rouhani, Amber N. Woods, Anthony B. Rodriguez et al.
    Frontiers in Immunology
  4. Role of IL-18 in atopic asthma is determined by balance of IL-18/IL-18BP/IL-18R
    Authors: H Zhang, J Wang, L Wang, H Xie, L Chen, S He
    J. Cell. Mol. Med., 2017-09-18;0(0):.
    Applications: Flow Cytometry Control

FAQs

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Reviews for Rat IgG1 APC-conjugated Isotype Control

Average Rating: 4 (Based on 1 Review)

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Rat IgG1 APC-conjugated Isotype Control
By Lu Bai on 07/26/2022
Application: Flow Sample Tested: bone marrow Species: Mouse