Rat IgG2B Isotype Control Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of Rat IgG Control by Flow Cytometry Human peripheral blood mononuclear cells (PBMCs) were stained with Rat Anti-Mouse CD44 Monoclonal Antibody (Catalog # MAB6127, filled histogram) or Rat IgG Isotype Control Antibody (Catalog #MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog F0105B).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IgG2B
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Product Datasheets
Citations for Rat IgG2B Isotype Control
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Turbinate-homing IgA-secreting cells originate in the nasal lymphoid tissues
Authors: Liu, J;Stoler-Barak, L;Hezroni-Bravyi, H;Biram, A;Lebon, S;Davidzohn, N;Kedmi, M;Chemla, M;Pilzer, D;Cohen, M;Brenner, O;Biton, M;Shulman, Z;
Nature
Species: Mouse
Sample Types: In Vivo
Applications: In vivo assay -
MIF-Modulated Spinal Proteins Associated with Persistent Bladder Pain: A Proteomics Study
Authors: Ye, S;Agalave, NM;Ma, F;Mahmood, DFD;Al-Grety, A;Khoonsari, PE;Leng, L;Svensson, CI;Bucala, R;Kultima, K;Vera, PL;
International journal of molecular sciences
Species: Mouse
Sample Types: In Vivo
Applications: In vivo assay -
CXCL5 suppression recovers neovascularization and accelerates wound healing in diabetes mellitus
Authors: Chen, C;Lin, LY;Chen, JW;Chang, TT;
Cardiovascular diabetology
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization Control -
GPR55 contributes to neutrophil recruitment and mechanical pain induction after spinal cord compression in mice
Authors: T Ono, T Yamashita, R Kano, M Inoue, S Okada, K Kano, S Koizumi, K Iwabuchi, Y Hirabayash, I Matsuo, Y Nakashima, H Kamiguchi, Y Kohro, M Tsuda
Brain, Behavior, and Immunity, 2023-03-08;110(0):276-287.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
GPR55 contributes to neutrophil recruitment and mechanical pain induction after spinal cord compression in mice
Authors: T Ono, T Yamashita, R Kano, M Inoue, S Okada, K Kano, S Koizumi, K Iwabuchi, Y Hirabayash, I Matsuo, Y Nakashima, H Kamiguchi, Y Kohro, M Tsuda
Brain, Behavior, and Immunity, 2023;110(0):276-287.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Inflammation triggers ILC3 patrolling of the intestinal barrier
Authors: A Jarade, Z Garcia, S Marie, A Demera, I Prinz, P Bousso, JP Di Santo, N Serafini
Nature Immunology, 2022-08-23;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
A functional role of meningeal lymphatics in sex difference of stress susceptibility in mice
Authors: W Dai, M Yang, P Xia, C Xiao, S Huang, Z Zhang, X Cheng, W Li, J Jin, J Zhang, B Wu, Y Zhang, PH Wu, Y Lin, W Wu, H Zhao, Y Zhang, WJ Lin, X Ye
Nature Communications, 2022-08-16;13(1):4825.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Microglia/macrophage-derived human CCL18 promotes glioma progression via CCR8-ACP5 axis analyzed in humanized slice model
Authors: Y Huang, E Motta, C Nanvuma, LD Kuhrt, Y Yuan, P Xia, M Lubas, S Zhu, M Schnauss, N Qazi, F Hu, H Zhang, T Lei, M Synowitz, C Flüh, H Kettenmann
Cell Reports, 2022-04-12;39(2):110670.
Species: Mouse
Sample Types: Tissue Culture Supernates
Applications: Neutralization Control -
Activation of the Innate Immune Checkpoint CLEC5A on Myeloid Cells in the Absence of Danger Signals Modulates Macrophages' Function but Does Not Trigger the Adaptive T Cell Immune Response
Authors: MJ Tosiek, K Groesser, A Pekcec, M Zwirek, G Murugesan, E Borges
Journal of Immunology Research, 2022-02-25;2022(0):9926305.
Species: Human
Sample Types: Whole Cells
Applications: Control -
Expression of IL-20 Receptor Subunit beta Is Linked to EAE Neuropathology and CNS Neuroinflammation
Authors: JR Dayton, Y Yuan, LP Pacumio, BG Dorflinger, SC Yoo, MJ Olson, SI Hernández-, MM McMahon, L Cruz-Oreng
Frontiers in Cellular Neuroscience, 2021-09-07;15(0):683687.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Microglial? signalling pathway deficits associated with the patient derived R47H TREM2 variants linked to AD indicate inability to activate inflammasome
Authors: K Cosker, A Mallach, J Limaye, TM Piers, J Staddon, SJ Neame, J Hardy, JM Pocock
Scientific Reports, 2021-06-25;11(1):13316.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Differentiation of induced pluripotent stem cells into definitive endoderm on Activin A-functionalized gradient surfaces
Authors: L Andreasson, H Evenbratt, R Mobini, S Simonsson
J Biotechnol, 2020-11-02;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
T cell-intrinsic role for Nod2 in protection against Th17-mediated uveitis
Authors: RJ Napier, EJ Lee, MP Davey, EE Vance, JM Furtado, PE Snow, KA Samson, SJ Lashley, BR Brown, R Horai, MJ Mattapalli, B Xu, MC Callegan, LS Uebelhoer, CL Lancioni, RK Vehe, BA Binstadt, JR Smith, RR Caspi, HL Rosenzweig
Nat Commun, 2020-10-26;11(1):5406.
Species: Mouse
Sample Types: In Vivo
Applications: Functional Assay -
Osteocyte necrosis triggers osteoclast-mediated bone loss through macrophage-inducible C-type lectin
Authors: D Andreev, M Liu, D Weidner, K Kachler, M Faas, A Grüneboom, U Schlötzer-, LE Muñoz, U Steffen, B Grötsch, B Killy, G Krönke, AM Luebke, A Niemeier, F Wehrhan, R Lang, G Schett, A Bozec
J. Clin. Invest., 2020-09-01;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay, Neutralization -
Tumor-infiltrating CD62L+PD-1-CD8 T cells retain proliferative potential via Bcl6 expression and replenish effector T cells within the tumor
Authors: Y Gong, T Suzuki, H Kozono, M Kubo, N Nakano
PLoS ONE, 2020-08-26;15(8):e0237646.
Species: Mouse
Sample Types: Whole Cells
Applications: Isotype Control -
Spatial proteomics revealed a CX3CL1-dependent crosstalk between the urothelium and relocated macrophages through IL-6 during an acute bacterial infection in the urinary bladder
Authors: Jenny Bottek, Camille Soun, Julia K. Lill, Akanksha Dixit, Stephanie Thiebes, Anna-Lena Beerlage et al.
Mucosal Immunology
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Dual‑targeting nanomicelles with CD133 and CD44 aptamers for enhanced delivery of gefitinib to two populations of lung cancer‑initiating cells
Authors: Xiaolong Huang, Jun Wan, Dewen Leng, Yingying Zhang, Shuo Yang
Experimental and Therapeutic Medicine
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Granulocyte Colony-Stimulating Factor Does Not Influence Clostridium Perfringens α-Toxin-Induced Myonecrosis in Mice
Authors: M Takehara, Y Sonobe, H Bandou, K Kobayashi, M Nagahama
Toxins (Basel), 2019-08-30;11(9):.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Interferon-?3 Promotes Epithelial Defense and Barrier Function Against Cryptosporidium parvum Infection
Authors: SH Ferguson, DM Foster, B Sherry, ST Magness, DM Nielsen, JL Gookin
Cell Mol Gastroenterol Hepatol, 2019-03-05;0(0):.
Species: Porcine
Sample Types: In Vivo
Applications: Neutralization -
The Chemokine Receptor CXCR4 Mediates Recruitment of CD11c+ Conventional Dendritic Cells Into the Inflamed Murine Cornea
Authors: MJ Lopez, Y Seyed-Raza, A Jamali, DL Harris, P Hamrah
Invest. Ophthalmol. Vis. Sci., 2018-11-01;59(13):5671-5681.
Species: Mouse
Sample Types: Whole Cells
Applications: Isotype Control -
Airway Epithelial Cell-Derived Colony Stimulating Factor-1 Promotes Allergen Sensitization
Authors: Hyung-Geun Moon, Seung-jae Kim, Jong Jin Jeong, Seon-Sook Han, Nizar N. Jarjour, Hyun Lee et al.
Immunity
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Integrin-based diffusion barrier separates membrane domains enabling formation of microbiostatic frustrated phagosomes
Authors: ME Maxson, X Naj, TR O'Meara, JD Plumb, LE Cowen, S Grinstein
Elife, 2018-03-19;7(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Control -
Imiquimod-Induced Psoriasis-Like Skin Lesions Do Not Accelerate Atherosclerosis in Low Density Lipoprotein Receptor-Deficient Mice
Authors: M Madsen, PR Hansen, LB Nielsen, RM Cardoso, M van Eck, TX Pedersen
Am. J. Pathol., 2018-03-12;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Toll-Interacting Protein, Tollip, Inhibits IL-13-Mediated Pulmonary Eosinophilic Inflammation in Mice
Authors: Y Ito, N Schaefer, A Sanchez, D Francisco, R Alam, RJ Martin, JG Ledford, C Stevenson, D Jiang, L Li, M Kraft, HW Chu
J Innate Immun, 2018-01-27;0(0):.
Species: Mouse
Sample Types: Whole Cells
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NKG2D ligand RAE1? induces generation and enhances the inhibitor function of myeloid-derived suppressor cells in mice
Authors: L Qian, Y Liu, S Wang, W Gong, X Jia, L Liu, F Ye, J Ding, Y Xu, Y Fu, F Tian
J. Cell. Mol. Med, 2017-03-09;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Smooth muscle cell-specific Tgfbr1 deficiency attenuates neointimal hyperplasia but promotes an undesired vascular phenotype for injured arteries
Physiol Rep, 2016-12-01;4(23):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Effect of 12-O-tetradecanoylphorbol-13-acetate-induced psoriasis-like skin lesions on systemic inflammation and atherosclerosis in hypercholesterolaemic apolipoprotein E deficient mice
Authors: Marie Madsen, Peter Riis Hansen, Lars Bo Nielsen, Karsten Hartvigsen, Anders Elm Pedersen, Jan Pravsgaard Christensen et al.
BMC Dermatology
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CCL19-CCR7-dependent reverse transendothelial migration of myeloid cells clears Chlamydia muridarum from the arterial intima
Nat Immunol, 2016-09-26;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Syndecan-1 (CD138) deficiency increases Staphylococcus aureus infection but has no effect on pathology in a mouse model of peritoneal dialysis.
Authors: Kowalewska P, Nguyen U, Burrows L, Fox-Robichaud A
J Biomed Sci, 2016-02-01;23(1):20.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Visualized macrophage dynamics and significance of S100A8 in obese fat.
Authors: Sekimoto R, Fukuda S, Maeda N, Tsushima Y, Matsuda K, Mori T, Nakatsuji H, Nishizawa H, Kishida K, Kikuta J, Maijima Y, Funahashi T, Ishii M, Shimomura I
Proc Natl Acad Sci U S A, 2015-04-06;112(16):E2058-66.
Species: Mouse
Sample Types: Whole Tissue
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Therapeutic exercise attenuates neutrophilic lung injury and skeletal muscle wasting
Authors: D. Clark Files, Chun Liu, Andrea Pereyra, Zhong-Min Wang, Neil R. Aggarwal, Franco R. D’Alessio et al.
Science Translational Medicine
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Degeneration and Regeneration of Corneal Nerves in Response to HSV-1 Infection
Authors: A. J. Chucair-Elliott, Min Zheng, Daniel J J Carr
Investigative Ophthalmology & Visual Science
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Structure-function analysis of CCL28 in the development of post-viral asthma.
Authors: Thomas M, Buelow B, Nevins A, Jones S, Peterson F, Gundry R, Grayson M, Volkman B
J Biol Chem, 2015-01-02;290(7):4528-36.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Mesenchymal Stem Cells Ameliorate Atherosclerotic Lesions via Restoring Endothelial Function.
Authors: Lin Y, Yet S, Hsu Y, Wang G, Hung S
Stem Cells Transl Med, 2014-12-10;4(1):44-55.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Nickel Nanoparticles cause exaggerated lung and airway remodeling in mice lacking the T-box transcription factor, TBX21 (T-bet)
Authors: Ellen E Glista-Baker, Alexia J Taylor, Brian C Sayers, Elizabeth A Thompson, James C Bonner
Particle and Fibre Toxicology
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Upregulation of Bone Morphogenetic Protein-1/Mammalian Tolloid and Procollagen C-Proteinase Enhancer-1 in Corneal Scarring
Authors: Francois Malecaze, Dawiyat Massoudi, Pierre Fournie, Cyrielle Tricoire, Myriam Cassagne, Marilyne Malbouyres et al.
Investigative Ophthalmology & Visual Science
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Syndecan-1 in the mouse parietal peritoneum microcirculation in inflammation.
Authors: Kowalewska P, Patrick A, Fox-Robichaud A
PLoS ONE, 2014-09-03;9(9):e104537.
Species: Mouse
Sample Types: In Vivo
Applications: IHC-Fr -
Oxidative stress induced age dependent meibomian gland dysfunction in Cu, Zn-superoxide dismutase-1 (Sod1) knockout mice.
Authors: Ibrahim O, Dogru M, Matsumoto Y, Igarashi A, Kojima T, Wakamatsu T, Inaba T, Shimizu T, Shimazaki J, Tsubota K
PLoS ONE, 2014-07-18;9(7):e99328.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
The cytokine midkine supports neutrophil trafficking during acute inflammation by promoting adhesion via beta2 integrins (CD11/CD18).
Authors: Weckbach L, Gola A, Winkelmann M, Jakob S, Groesser L, Borgolte J, Pogoda F, Pick R, Pruenster M, Muller-Hocker J, Deindl E, Sperandio M, Walzog B
Blood, 2014-01-23;123(12):1887-96.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Monocytes Control Second-Phase Neutrophil Emigration in Established Lipopolysaccharide-induced Murine Lung Injury
Authors: Kevin Dhaliwal, Emma Scholefield, David Ferenbach, Michael Gibbons, Rodger Duffin, David A. Dorward et al.
American Journal of Respiratory and Critical Care Medicine
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The role of SDF-1-CXCR4/CXCR7 axis in the therapeutic effects of hypoxia-preconditioned mesenchymal stem cells for renal ischemia/reperfusion injury.
Authors: Liu H, Liu S, Li Y, Wang X, Xue W, Ge G, Luo X
PLoS ONE, 2012-04-12;7(4):e34608.
Species: Mouse
Sample Types: Whole Cells
Applications: Control -
E-cadherin-expressing feeder cells promote neural lineage restriction of human embryonic stem cells.
Authors: Moore RN, Cherry JF, Mathur V
Stem Cells Dev., 2011-06-01;21(1):30-41.
Species: Mouse
Sample Types: Whole Cells
Applications: Control -
IL-13 induces skin fibrosis in atopic dermatitis by thymic stromal lymphopoietin.
Authors: Oh MH, Oh SY, Yu J
J. Immunol., 2011-05-16;186(12):7232-42.
Species: Mouse
Sample Types: In Vivo
Applications: Control -
IL-21 Promotes Skin Recruitment of CD4+ Cells and Drives IFN-{gamma}-Dependent Epidermal Hyperplasia.
Authors: Sarra M, Caruso R, Cupi ML, Monteleone I, Stolfi C, Campione E, Diluvio L, Mazzotta A, Botti E, Chimenti S, Costanzo A, MacDonald TT, Pallone F, Monteleone G
J. Immunol., 2011-03-25;186(9):5435-42.
Species: Mouse
Sample Types: In Vivo
Applications: Control -
Mechanical Stretch Induces Epithelial-Mesenchymal Transition in Alveolar Epithelia via Hyaluronan Activation of Innate Immunity.
Authors: Heise RL, Stober V, Cheluvaraju C, Hollingsworth JW, Garantziotis S
J. Biol. Chem., 2011-03-11;286(20):17435-44.
Species: Mouse
Sample Types: Whole Cells
Applications: Control -
Overexpression of sICAM-1 in the Alveolar Epithelial Space Results in an Exaggerated Inflammatory Response and Early Death in Gram Negative Pneumonia.
Authors: Mendez MP, Monroy YK, Du M
Respir. Res., 2011-01-19;12(1):12.
Species: Mouse
Sample Types: BALF
Applications: Control -
Dynamic macrophage "probing" is required for the efficient capture of phagocytic targets.
Authors: Flannagan RS, Harrison RE, Yip CM, Jaqaman K, Grinstein S
J. Cell Biol., 2010-12-06;191(6):1205-18.
Species: Mouse
Sample Types:
Applications: Flow Cytometry Control -
Breast milk immune complexes are potent inducers of oral tolerance in neonates and prevent asthma development.
Authors: Mosconi E, Rekima A, Seitz-Polski B
Mucosal Immunol, 2010-05-19;3(5):461-74.
Species: Mouse
Sample Types: In Vivo
Applications: Control -
Chlamydial respiratory infection during allergen sensitization drives neutrophilic allergic airways disease.
Authors: Horvat JC, Starkey MR, Kim RY, Beagley KW, Preston JA, Gibson PG, Foster PS, Hansbro PM
J. Immunol., 2010-03-12;184(8):4159-69.
Species: Mouse
Sample Types: In Vivo
Applications: Control -
Microarray analysis of retinal endothelial tip cells identifies CXCR4 as a mediator of tip cell morphology and branching.
Authors: Strasser GA, Kaminker JS, Tessier-Lavigne M
Blood, 2010-02-12;115(24):5102-10.
Species: Mouse
Sample Types: In Vivo
Applications: Control -
A human colonic commensal promotes colon tumorigenesis via activation of T helper type 17 T cell responses.
Authors: Wu S, Rhee KJ, Albesiano E, Rabizadeh S, Wu X, Yen HR, Huso DL, Brancati FL, Wick E, McAllister F, Housseau F, Pardoll DM, Sears CL
Nat. Med., 2009-08-23;15(9):1016-22.
Species: Mouse
Sample Types: In Vivo
Applications: Control -
MCP-1 antibody treatment enhances damage and impedes repair of the alveolar epithelium in influenza pneumonitis.
Authors: Narasaraju T, Ng HH, Phoon MC
Am. J. Respir. Cell Mol. Biol., 2009-07-17;42(6):732-43.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
A new RXR agonist, HX630, suppresses intimal hyperplasia in a mouse blood flow cessation model.
Authors: Haraguchi G, Suzuki J, Kosuge H, Ogawa M, Koga N, Muto S, Itai A, Kagechika H, Shudo K, Isobe M
J. Mol. Cell. Cardiol., 2006-09-11;41(5):885-92.
Species: Mouse
Sample Types: Whole Tissue
Applications: Control -
CCL27 is a critical factor for the development of atopic dermatitis in the keratin-14 IL-4 transgenic mouse model.
Authors: Chen L, Lin SX, Agha-Majzoub R, Overbergh L, Mathieu C, Chan LS
Int. Immunol., 2006-05-30;18(8):1233-42.
Species: Mouse
Sample Types:
Applications: Control -
Shedding of soluble ICAM-1 into the alveolar space in murine models of acute lung injury.
Authors: Mendez MP, Morris SB, Wilcoxen S, Greeson E, Moore B, Paine R
Am. J. Physiol. Lung Cell Mol. Physiol., 2005-12-22;290(5):L962-70.
Species: Mouse
Sample Types: Cell Lysates
Applications: Control -
Transcriptional profiling of lipopolysaccharide-induced acute lung injury.
Authors: Jeyaseelan S, Chu HW, Young SK, Worthen GS
Infect. Immun., 2004-12-01;72(12):7247-56.
Species: Mouse
Sample Types: In Vivo, Whole Cells
Applications: Control, Neutralization -
Rapid up-regulation of CXC chemokines in the airways after Ag-specific CD4+ T cell activation.
Authors: Knott PG, Gater PR, Dunford PJ, Fuentes ME, Bertrand CP
J. Immunol., 2001-01-15;166(2):1233-40.
Species: Mouse
Sample Types: In Vivo
Applications: Control
FAQs
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What is the light chain of Rat IgG2B Isotype Control, Catalog # MAB0061?
The light chain is kappa.
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