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Rat ICAM-1/CD54 Antibody

Catalog #: MAB5832
2 Citations Datasheet
Catalog # Availability Size / Price Qty
MAB5832-SP
MAB5832-500
MAB5832-100
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat ICAM-1/CD54 Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat ICAM-1 in ELISAs and Western blots. Does not cross-react with recombinant human (rh) CD31, recombinant mouse (rm) DCC, rhICAM-1, rmICAM-1, rmICAM-2, rhICAM-3, rhMAdCAM-1, or rmVCAM-1.
Source
Monoclonal Mouse IgG1 Clone # 141017
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat ICAM-1
Met1-Thr493
Accession # Q00238
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample

Rat ICAM-1/CD54 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Rat ICAM‑1/CD54 Biotinylated Antibody (Catalog # BAF583)

Standard: Recombinant Rat ICAM-1/CD54 Fc Chimera Protein, CF (Catalog # 583-IC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-1/CD54

Intercellular Adhesion Molecule-1 (ICAM-1, CD54), binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS. Rat and human ICAM-1 share approximately 52% amino acid identity. Rat and mouse ICAM-1 share approximately 52% amino acid identity.

Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer and transplantation.

References
  1. Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book, p. 74. Academic Press.
  2. Siu, G. et al. (1989) J. Immunol. 143:3813.
  3. Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853.
Long Name
Intercellular Adhesion Molecule 1
Entrez Gene IDs
3383 (Human); 15894 (Mouse); 25464 (Rat)
Alternate Names
BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58

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Citations for Rat ICAM-1/CD54 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Efficacy of Antibody Delivery to the Retina and Optic Nerve by Topical Administration
    Authors: Stacy Hu, Steven Koevary
    Journal of Ocular Pharmacology and Therapeutics
  2. Intravenous injection of post‑hemorrhagic shock mesenteric lymph induces multiple organ injury in rats
    Authors: Yifeng Zhao, Limin Zhang, Rui Han, Yonghua Si, Zigang Zhao
    Experimental and Therapeutic Medicine

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