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Rat Glut4 Alexa Fluor® 350-conjugated Antibody

Catalog #: FAB1262U Datasheet
Catalog # Availability Size / Price Qty
FAB1262U-100UG
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Rat Glut4 Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity
Rat
Specificity
Recognizes an epitope in the cytoplasmic portion of rat Glut4 and has been shown to bind only to the muscle-adipose isoform of the glucose transporter, which is antigenically unique from the other transport proteins (1-3). Recognizes Glut4 in human, monke
Source
Monoclonal Mouse IgG1 Clone # 1F8
Purification
Protein A or G purified
Immunogen
Partially purified vesicles containing Glut4
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
 
Immunoprecipitation
Optimal dilution of this antibody should be experimentally determined.
 
Immunoaffinity Purification
Optimal dilution of this antibody should be experimentally determined.
 
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Glut4

Glut4 belongs to the facilitative glucose transporter protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains. Glut4 is expressed in the insulin-responsive tissues, muscle and adipose tissue. Insulin stimulation leads to translocation of Glut4 from intracellular stores to the cell surface resulting in the enhancement of glucose uptake.

Entrez Gene IDs
6517 (Human); 20528 (Mouse); 25139 (Rat)
Alternate Names
Glut4; GLUT-4; GLUT4Glucose transporter type 4, insulin-responsive; insulin-responsive glucose transporter type 4; SLC2A4; solute carrier family 2 (facilitated glucose transporter), member 4; solute carrier family 2, facilitated glucose transporter member 4

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