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Rat CXCL2/CINC-3 Quantikine ELISA Kit

Catalog #: RCN300
17 Citations Datasheet
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RCN300
Rat CXCL2/GRO beta/MIP-2/CINC-3 ELISA Standard Curve
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Citations (17)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Rat CXCL2/CINC-3 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
2.7 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant rat CINC-3
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Rat CINC-3 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure rat CINC-3 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rat CINC-3 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural rat CINC-3 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring rat CINC-3.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 50 46 50
Mean (pg/mL) 111 345 1012 105 308 877
Standard Deviation 3.9 11.9 39.1 10.1 28.5 73.2
CV% 3.5 3.4 3.9 9.6 9.3 8.3

Recovery

The recovery of rat CINC-3 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 97 86-107
EDTA Plasma (n=6) 92 86-99
Heparin Plasma (n=6) 99 85-105
Serum (n=6) 97 83-106

Linearity

To assess the linearity of the assay, samples containing rat CINC-3 in each matrix were diluted with Calibrator Diluent and then assayed.
Rat CXCL2/GRO beta/MIP-2/CINC-3 ELISA Linearity

Scientific Data

N/A Rat CXCL2/GRO beta/MIP-2/CINC-3 ELISA Standard Curve View Larger

Rat CXCL2/GRO beta/MIP-2/CINC-3 ELISA Standard Curve

Product Datasheets

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Product Datasheet

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL2/GRO beta/MIP-2/CINC-3

CXCL2/GRO beta, also called MIP-2 in mouse and CINC-3 in rat, is a member of the CXC chemokine family. Human CXCL2/GRO beta is 107 amino acids (aa) in length with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 70% and 71% aa sequence identity with the human protein, respectively. N-terminal aa 1-4 of CXCL2/GRO beta can be post-translationally cleaved which confers enhanced hematopoietic bioactivity. CXCL2/GRO beta is produced by a variety of cell types including monocytes and macrophages at sites of inflammation and is chemotactic for granulocytes, including neutrophils.

Entrez Gene IDs:
2920 (Human); 20310 (Mouse); 114105 (Rat)
Alternate Names:
chemokine (C-X-C motif) ligand 2; CINC-2a; CINC3; CINC-3; C-X-C Motif Chemokine 2; CXCL2; GRO beta; GRO2 oncogene; GRO2; GROB; Gro-beta; Growth-regulated protein beta; Macrophage inflammatory protein 2-alpha; melanoma growth stimulatory activity beta; MGSA beta; MGSA-b; MGSA-beta; MIP2; MIP-2; MIP2A; MIP-2a; MIP2-alpha; SCYB2
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 5 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Rat CXCL2/CINC-3 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Schwann cell-derived CXCL2 contributes to cancer pain by modulating macrophage infiltration in a mouse breast cancer model
    Authors: Y Zhang, R Sang, J Bao, Z Jiang, D Qian, Y Zhou, W Su, J Wei, L Zhao, Z Wei, Y Zhao, M Shi, G Chen
    Brain, Behavior, and Immunity, 2023-02-06;0(0):.
    Species: Rat
    Sample Types: Cell Culture Supernates
  2. Dapsone Azo-Linked with Two Mesalazine Moieties Is a "Me-Better" Alternative to Sulfasalazine
    Authors: C Kang, J Kim, S Ju, S Park, JW Yoo, IS Yoon, MS Kim, Y Jung
    Pharmaceutics, 2022-03-21;14(3):.
    Species: Rat
    Sample Types: Tissue Homogenates
  3. The development of various forms of lung injury with increasing tidal volume in normal rats
    Authors: E D'Angelo, A Koutsoukou, P Della Vall, G Gentile, M Pecchiari
    Respir Physiol Neurobiol, 2020-01-03;274(0):103369.
    Species: Rat
    Sample Types: Serum
  4. Dexamethasone-induced Intra-Uterine Growth Restriction impacts NOSTRIN and its downstream effector genes in the rat mesometrial uterus
    Authors: S Chakrabort, S Islam, S Saha, R Ain
    Sci Rep, 2018-05-29;8(1):8342.
    Species: Human
    Sample Types: Tissue Homogenates
  5. Assessment of Pulmonary Toxicity Induced by Inhaled Toner with External Additives
    Authors: T Tomonaga, H Izumi, Y Yoshiura, T Myojo, T Oyabu, BW Lee, T Okada, Y Li, K Kawai, T Higashi, Y Morimoto
    Biomed Res Int, 2017-01-16;2017(0):4245309.
    Species: Mouse
    Sample Types: Tissue Homogenates
  6. NF-kappaB and STAT1 control CXCL1 and CXCL2 gene transcription.
    Authors: Burke S, Lu D, Sparer T, Masi T, Goff M, Karlstad M, Collier J
    Am J Physiol Endocrinol Metab, 2013-11-26;306(2):E131-49.
    Species: Rat
    Sample Types: Cell Culture Supernates
  7. Mechanical ventilation enhances HMGB1 expression in an LPS-induced lung injury model.
    Authors: Ding N, Wang F, Xiao H, Xu L, She S
    PLoS ONE, 2013-09-10;8(9):e74633.
    Species: Rat
    Sample Types: BALF
  8. A short course of infusion of a hydrogen sulfide-donor attenuates endotoxemia induced organ injury via stimulation of anti-inflammatory pathways, with no additional protection from prolonged infusion.
    Cytokine, 2012-12-23;61(2):614-21.
    Species: Rat
    Sample Types: BALF
  9. P2X7 receptor activation induces CXCL2 production in microglia through NFAT and PKC/MAPK pathways.
    Authors: Shiratori M, Tozaki-Saitoh H, Yoshitake M
    J. Neurochem., 2010-05-13;114(3):810-9.
    Species: Rat
    Sample Types: Cell Culture Supernates
  10. Mechanism of hepatoprotection in proestrus female rats following trauma-hemorrhage: heme oxygenase-1-derived normalization of hepatic inflammatory responses.
    Authors: Yang S, Hu S, Chen J, Choudhry MA, Rue LW, Bland KI, Chaudry IH
    J. Leukoc. Biol., 2009-02-24;85(6):1015-26.
    Species: Rat
    Sample Types: Serum
  11. Antithrombin inhibits bronchoalveolar activation of coagulation and limits lung injury during Streptococcus pneumoniae pneumonia in rats.
    Authors: Choi G, Hofstra JJ, Roelofs JJ, Rijneveld AW, Bresser P, van der Zee JS, Florquin S, van der Poll T, Levi M, Schultz MJ
    Crit. Care Med., 2008-01-01;36(1):204-10.
    Species: Rat
    Sample Types: BALF
  12. ACE mediates ventilator-induced lung injury in rats via angiotensin II but not bradykinin.
    Authors: Wosten-van Asperen RM, Lutter R, Haitsma JJ, Merkus MP, van Woensel JB, van der Loos CM, Florquin S, Lachmann B, Bos AP
    Eur. Respir. J., 2007-10-24;31(2):363-71.
    Species: Rat
    Sample Types: BALF
  13. Mechanism of the nongenomic effects of estrogen on intestinal myeloperoxidase activity following trauma-hemorrhage: up-regulation of the PI-3K/Akt pathway.
    Authors: Yu HP, Hsieh YC, Suzuki T, Choudhry MA, Schwacha MG, Bland KI, Chaudry IH
    J. Leukoc. Biol., 2007-06-22;82(3):774-80.
    Species: Rat
    Sample Types: Tissue Homogenates
  14. Natural anticoagulants limit lipopolysaccharide-induced pulmonary coagulation but not inflammation.
    Authors: Choi G, Vlaar AP, Schouten M, Van't Veer C, van der Poll T, Levi M, Schultz MJ
    Eur. Respir. J., 2007-05-30;30(3):423-8.
    Species: Rat
    Sample Types: Tissue Homogenates
  15. Anti-rat soluble IL-6 receptor antibody down-regulates cardiac IL-6 and improves cardiac function following trauma-hemorrhage.
    Authors: Yang S, Hu S, Choudhry MA, Rue LW, Bland KI, Chaudry IH
    J. Mol. Cell. Cardiol., 2006-12-16;42(3):620-30.
    Species: Rat
    Sample Types: Tissue Homogenates
  16. Maintenance of lung myeloperoxidase activity in proestrus females after trauma-hemorrhage: upregulation of heme oxygenase-1.
    Authors: Yu HP, Yang S, Hsieh YC, Choudhry MA, Bland KI, Chaudry IH
    Am. J. Physiol. Lung Cell Mol. Physiol., 2006-03-23;291(3):L400-6.
    Species: Rat
    Sample Types: Tissue Homogenates
  17. Mechanism of IL-6-mediated cardiac dysfunction following trauma-hemorrhage.
    Authors: Yang S, Hu S, Hsieh YC, Choudhry MA, Rue LW, Bland KI, Chaudry IH
    J. Mol. Cell. Cardiol., 2006-02-24;40(4):570-9.
    Species: Rat
    Sample Types: Tissue Homogenates

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