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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Catalog #: F0133
1 Citation Datasheet
Catalog # Availability Size / Price Qty
F0133
Detection of Mouse IgG2BPrimary Antibody by Flow Cytometry.
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Rat Anti-Mouse IgG2B APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
The specificity of this antibody was confirmed by direct ELISA on mouse IgG2B monoclonal antibodies, and it does not cross-react with IgG1, IgG2A, IgG3, IgM, IgA, or IgE antibodies. By flow cytometry, this isotype-specific secondary antibody detects primary antibodies of the mouse IgG2B isotype, and does not detect primary antibodies of mouse IgG1, IgG2A, or IgG3 class.
Source
Monoclonal Rat IgG1 Clone # 332723
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Cocktail of mouse IgG2B and IgG3
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Mouse IgG<SUB>2B</SUB>Primary Antibody by Flow Cytometry. View Larger

Detection of Mouse IgG2BPrimary Antibody by Flow Cytometry. Human peripheral blood lymphocytes were stained with A) Mouse Anti-Human CD8a Monoclonal Antibody (Catalog # MAB1509, Mouse IgG2B, filled histogram) or isotype control (Catalog # MAB0041, open histogram), or B) Mouse Anti-Human CD4 Monoclonal Antibody (Catalog # MAB3791, Mouse IgG2A, filled histogram) or isotype control (Catalog # MAB0041, open histogram), followed by APC-conjugated Rat Anti-Mouse IgG2Bisotype-specific secondary antibody (Catalog # F0133). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2B Fc
Alternate Names
IgG2B

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Citation for Rat Anti-Mouse IgG2B APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. An in vitro model of antibody-mediated injury to glomerular endothelial cells: Upregulation of MHC class II and adhesion molecules
    Authors: Nancy A. Wilson, James Dylewski, Kenna R. Degner, Megan A. O'Neill, Shannon R. Reese, Luis G. Hidalgo et al.
    Transplant Immunology

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