Rat Adiponectin/Acrp30 Antibody Summary
Met1-Asn244
Accession # NP_653345
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Rat Adiponectin/Acrp30 by Western Blot. Western blot shows lysates of rat adipose tissue and rat mammary gland tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Rat Adiponectin/Acrp30 Monoclonal Antibody (Catalog # MAB3100) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Adiponectin/Acrp30 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Adiponectin/Acrp30 in Rat Adipocytes. Adiponectin/Acrp30 was detected in Immersion fixed rat mesenchymal stem cells differentiated into adipocytes with Human/Mouse/Rat StemXVivo Adipogenic Supplement (Catalog # CCM011) using Mouse Anti-Rat Adiponectin/Acrp30 Monoclonal Antibody (Catalog # MAB3100) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Adiponectin/Acrp30
Adiponectin, also known as Adipocyte complement-related protein of 30 kDa (Acrp30), is a secreted adipose tissue-specific protein. It is an anti-atherogenic, anti-diabetic and anti-inflammatory hormone that plays important roles in the regulation of lipid and glucose metabolism. Rat Adiponectin/Acrp30 shares 91% and 83% amino acid sequence homology with mouse and human Adiponectin/Acrp30, respectively.
Product Datasheets
Citation for Rat Adiponectin/Acrp30 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Therapeutic potential of epiphyseal growth plate cells for bone regeneration in an osteoporosis model
Authors: I Baek, AB Bello, J Jeon, Y Arai, BH Cha, BJ Kim, SH Lee
Journal of tissue engineering, 2022-08-11;13(0):2041731422111.
Species: Rat
Sample Types: Cell Lysate
Applications: Western Blot
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