Rabbit VisUCyte HRP Polymer-DAB Cell & Tissue Staining Kit
Rabbit VisUCyte HRP Polymer-DAB Cell & Tissue Staining Kit Summary
Key Benefits
VisUCyte kit outperforms conventional Avidin-Biotin-HRP detection by providing the following advantages:
- High-sensitivity detection: Detect low-abundant protein targets, saving costs with reduced primary antibody usage.
- Lack of non-specific staining: Specific signal stays clear without interference from irrelevant staining profiles.
- Avidin and biotin-free: Skip the need to quench endogenous biotin, preventing non-specific tissue staining.
- Swift protocol: Our time-efficient staining procedure accelerates scientific data generation.
- Ready-to-use HRP polymer conjugated secondary antibodies: No hassle with staining optimization.
- Convenient packaging: Offers easy handling and no confusion with kit components.
Our HRP polymer-conjugated secondary antibodies are versatile, reacting with all IgG subclasses, suitable for both manual protocols and tissue autostainers. Secondary antibodies used for conjugation to HRP polymer undergo rigorous quality control, ensuring reproducibility. Due to their high sensitivity, VisUCyte™ kits are ideal for detecting low-abundant tissue proteins, providing the ability to complete IHC experiments in just 1-2 hours, while additionally resulting in significant cost savings.
Download the datasheet to read full technical specifications, staining procedure, and more.
Specifications
Product Datasheets
Scientific Data
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Detection of Ki67/MKI67 in Kidney Tumor. Tissue sections were first incubated with Human Ki67/MKI67 Antibody (MAB7617) and then subjected to staining development using Rabbit IgG VisUCyte HRP-DAB Kit (Catalog # VCTS003B). Specific staining was localized to the nucleus. Note strong and high contrast specific tissue staining and the absence of non-specific background staining produced by the VisUCyte kit.
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Detection of ErbB2/Her2 in human breast cancer Tissue sections were first incubated with Rabbit Anti-Human Phospho-ErbB2/Her2 Polyclonal Antibody (AF1768) and then subjected to staining development using Rabbit VisUCyte Detection kit (Catalog# VCTS003B). Staining is confined to the cell membranes of cancer cells. Note strong and high contrast specific tissue staining and the absence of non-specific background staining produced by the VisUCyte kit.
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Side-by-side comparison of staining using different detection chemistries on adjacent tissue sections of Human Colon Tumor. Ki67/MKI67 was detected in immersion fixed paraffin-embedded sections of human colon tumor using Rabbit Anti-Human Ki67/MKI67 Monoclonal Antibody (Catalog # MAB7617) at 1 µg/ml for overnight at 4 °C followed by incubation with the Rabbit VisUCyte HRP Polymer-DAB Cell & Tissue Staining Kit (Catalog # VCTS003B) and a generic Avidin-Biotin kit. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. Strong specific tissue staining is seen using VisUCyte kit. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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