Rabbit TNF-alpha Antibody

Catalog #: MAB56701
1 Citation Datasheet

Catalog #	Availability	Size / Price	Qty
MAB56701
MAB56701-SP

All TNF-alpha Antibodies

Product Details

Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rabbit TNF-alpha Antibody Summary

Species Reactivity

Rabbit

Specificity

Detects rabbit TNF-alpha in direct ELISAs. In direct ELISAs, 50-100% cross-reactivity with recombinant cotton rat, feline, guinea pig, human, mouse, rat TNF-alpha, less than 10% cross-reactivity with recombinant canine, equine, rhesus TNF-alpha, and no cross-reactivity with recombinant bovine or porcine TNF-alpha is observed. No cross-reactivity with recombinant human (rh) 4-1BB Ligand, APRIL, rhBAFF, rhEDA, rhEDA-A2, rhFas Ligand, rhGITR Ligand, rhLIGHT, rhLymphotoxin alpha 1 beta 2, rhLymphotoxin alpha 2 beta 1, rhOX40 Ligand, rhTRAIL, rhTRANCE, rhTWEAK, rhVEGI, recombinant mouse (rm) 4-1BB Ligand, rmBAFF, rmFas Ligand, rmGITR Ligand, rmLIGHT, rmOX40 Ligand, rmTRAIL, rmTRAIL-2 Ligand, rmTRANCE, or rmTWEAK is observed.

Source

Monoclonal Mouse IgG_2B Clone # 610325

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant rabbit TNF-alpha
Leu79-Leu234
Accession # P04924

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Rabbit TNF-alpha (Catalog # 5670-TG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TNF-alpha

Tumor necrosis factor (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily and family. The 26 kDa type II transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Rabbit TNF-alpha is 235 amino acids (aa) in length and contains a 35 aa cytoplasmic domain, a 21 aa transmembrane region, and a 179 extracellular domain (ECD).Cleavage of membrane bound TNF-alpha by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-alpha. Rabbit TNF-alpha is 79% and 78% aa identical to human and mouse TNF-alpha, respectively. TNF-alpha is produced by several lymphoid cells as well as by astrocytes, endothelial cells, and smooth muscle cells. TNF-alpha binds TNF RI and TNF RII present on virtually all cell types where it triggers the activation of multiple signal transduction pathways and modulates the expression of a wide variety of genes.

Long Name

Tumor Necrosis Factor alpha

Entrez Gene IDs

7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 102139631 (Cynomolgus Monkey); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)

Alternate Names

APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha

Product Datasheets

Product Datasheet

Related Research Areas

Citation for Rabbit TNF-alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1