Rabbit IgG Biotinylated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Detection of Rabbit IgG Antibody by Simple Western<sup>TM</sup>. Detection of Rabbit IgG Antibody by Simple Western<sup>TM</sup>.](https://resources.rndsystems.com/images/datasheets/antibody/IgG_BAF008_Simple_Western_22638.jpg)
Detection of Rabbit IgG Antibody by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Survivin at approximately 24 kDa (as indicated) using 5 µg/mL (Lane A), 1.25 µg/mL (Lane B), or 0 µg/mL Lane C, negative control) of Rabbit Anti-Human Survivin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF886) followed by 1:500 dilution of Biotin-conjugated Anti-Rabbit IgG Secondary Antibody (BAF008). Streptavidin-HRP was used for detection. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
![Detection of Rabbit IgG Antibody by Simple Western<sup>TM</sup>. Detection of Rabbit IgG Antibody by Simple Western<sup>TM</sup>.](https://resources.rndsystems.com/images/datasheets/antibody/IgG_BAF008_Simple_Western_22639.jpg)
Detection of Rabbit IgG Antibody by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP70/HSPA1A at approximately 71 kDa (as indicated) using 5 µg/mL (Lane A), 1.25 µg/mL (Lane B), or 0 µg/mL Lane C, negative control) of Rabbit Anti-Human/Mouse/Rat HSP70/HSPA1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1663) followed by 1:500 dilution of Biotin-conjugated Anti-Rabbit IgG Secondary Antibody (BAF008). Streptavidin-HRP was used for detection. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Product Datasheets
Citations for Rabbit IgG Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
11
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
Silencing Tspan1 inhibits migration and invasion, and induces the apoptosis of human pancreatic cancer cells
Authors: Tian J, Zhang R, Piao H et al.
Mol Med Rep
-
Inhibition of Ezrin suppresses cell migration and invasion in human nasopharyngeal carcinoma.
Authors: Tang Y, Sun X, et al.
Oncol Lett
-
Optogenetic manipulation of BMP signaling to drive chondrogenic differentiation of hPSCs
Authors: Humphreys, PEA;Woods, S;Bates, N;Rooney, KM;Mancini, FE;Barclay, C;O'Flaherty, J;Martial, FP;Domingos, MAN;Kimber, SJ;
Cell reports
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
The preventive effects of probiotic Akkermansia muciniphila on D-galactose/AlCl3 mediated Alzheimer's disease-like rats
Authors: X He, C Yan, S Zhao, Y Zhao, R Huang, Y Li
Experimental gerontology, 2022-09-21;0(0):111959.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
TSPAN1 upregulates MMP2 to promote pancreatic cancer cell migration and invasion via PLC?
Authors: X Zhang, G Shi, F Gao, P Liu, H Wang, X Tan
Oncol. Rep., 2019-01-30;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
An insight into the role of magnesium in the immunomodulatory properties of mesenchymal stem cells
Authors: F da Silva L, AB da Rocha R, A Hastreiter, A Nogueira-P, E Makiyama, C Colli, RA Fock
J. Nutr. Biochem., 2018-02-13;55(0):200-208.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
The expression of SIRT1 regulates the metastaticplasticity of chondrosarcoma cells by inducing epithelial-mesenchymal transition
Authors: H Feng, J Wang, J Xu, C Xie, F Gao, Z Li
Sci Rep, 2017-01-23;7(0):41203.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Effect of hyperinsulinemia during hemodialysis on the insulin-like growth factor system and inflammatory biomarkers: a randomized open-label crossover study.
Authors: Reinhard M, Frystyk J, Jespersen B, Bjerre M, Christiansen J, Flyvbjerg A, Ivarsen P
BMC Nephrol, 2013-04-04;14(0):80.
Species: Human
Sample Types: Serum, Whole Cells
Applications: ELISA Development, Functional Assay -
Human corneal epithelium-derived thymic stromal lymphopoietin links the innate and adaptive immune responses via TLRs and Th2 cytokines.
Authors: Ma P, Bian F, Wang Z, Zheng X, Chotikavanich S, Pflugfelder SC, Li DQ
Invest. Ophthalmol. Vis. Sci., 2009-01-17;50(6):2702-9.
Species: Human
Sample Types: Antibody
Applications: IHC-Fr -
A novel role of the lumican core protein in bacterial lipopolysaccharide-induced innate immune response.
Authors: Wu F, Vij N, Roberts L, Lopez-Briones S, Joyce S, Chakravarti S
J. Biol. Chem., 2007-07-05;282(36):26409-17.
Species: Mouse
Sample Types: Cell Lysates
Applications: ELISA Development -
Effects of three IL-15 variants on NCI-H446 cell proliferation and expression of cell cycle regulatory molecules
Authors: Jun-Ying Ding, Zhi-Hua Wang, Zheng-Zheng Zhang, Xu-Ran Cui, Yan-Ying Hong, Qing-Quan Liu
Oncotarget
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Rabbit IgG Biotinylated Antibody
Average Rating: 4.3 (Based on 3 Reviews)
Have you used Rabbit IgG Biotinylated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by: