Proteome Profiler Mouse Adipokine Array Kit
Proteome Profiler Mouse Adipokine Array Kit Summary
To simultaneously detect the relative levels of 38 different obesity-related molecules in a single sample. No specialized equipment is necessary.
- Product Insert
- Troubleshooting Guide
- Assays for analytes represented in the Mouse Adipokine Array Kit
General Assay Principle
Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernatant, cell lysate, tissue lysate, or serum samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any cytokine/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of cytokine bound. Chemiluminescence is detected in the same manner as a Western blot.
- 4 Array Membranes
- 4-Well Multi-dish
- Array Buffers
- Wash Buffer
- Detection Antibody Cocktail
- Streptavidin-HRP
- Chemiluminescent Detection Reagents
- Transparency Overlay Template
- Detailed Protocol
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Materials Required sections of the product datasheet.
Stability and Storage
Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.
Simultaneously detect the levels of these obesity-related proteins in a single sample. | ||
Adiponectin | IGF-I | M-CSF |
AgRP | IGF-II | OSM |
ANGPT-L3 | IGFBP-1 | PAI-1/Serpin E1 |
CCL2/MCP-1/JE | IGFBP-2 | PTX2 |
CCL5/RANTES | IGFBP-3 | PTX3 |
CRP | IGFBP-5 | Pref-1 |
DPPIV/CD26 | IGFBP-6 | RAGE |
Endocan | IL-6 | RBP4 |
Fetuin A | IL-10 | Resistin |
FGF acidic | IL-11 | TIMP-1 |
FGF-21 | Leptin | TNF-alpha |
HGF | LIF | VEGF |
ICAM-1 | Lipocalin-2/NGAL |
Specifications
Product Datasheets
Citations for Proteome Profiler Mouse Adipokine Array Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Novel role of Quercetin in ameliorating metabolic syndrome via VDR mediated activation of adiponectin/AdipoR2 signaling
Authors: Sannappa Gowda, NG;Shiragannavar, VD;Karunakara, SH;Veeranna, RP;Suvarna, D;Kumar, DP;Santhekadur, PK;
Biochemistry and biophysics reports 2024-09-01
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Identification of IGF-1 Effects on White Adipose Tissue and Hippocampus in Alzheimer's Disease Mice via Transcriptomic and Cellular Analysis
Authors: Kim, YK;Jo, D;Arjunan, A;Ryu, Y;Lim, YH;Choi, SY;Kim, HK;Song, J;
International journal of molecular sciences 2024-02-22
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Adipocyte p53 coordinates the response to intermittent fasting by regulating adipose tissue immune cell landscape
Authors: Reinisch, I;Michenthaler, H;Sulaj, A;Moyschewitz, E;Krstic, J;Galhuber, M;Xu, R;Riahi, Z;Wang, T;Vujic, N;Amor, M;Zenezini Chiozzi, R;Wabitsch, M;Kolb, D;Georgiadi, A;Glawitsch, L;Heitzer, E;Schulz, TJ;Schupp, M;Sun, W;Dong, H;Ghosh, A;Hoffmann, A;Kratky, D;Hinte, LC;von Meyenn, F;Heck, AJR;Blüher, M;Herzig, S;Wolfrum, C;Prokesch, A;
Nature communications 2024-02-15
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High-fat diet induces C-reactive protein secretion, promoting lung adenocarcinoma via immune microenvironment modulation
Authors: Hsu, WL;Hsieh, YT;Chen, WM;Chien, MH;Luo, WJ;Chang, JH;Devlin, K;Su, KY;
Disease models & mechanisms 2023-11-01
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Oncogenic KRAS drives lipo-fibrogenesis to promote angiogenesis and colon cancer progression
Authors: Hsu, WH;LaBella, KA;Lin, Y;Xu, P;Lee, R;Hsieh, CE;Yang, L;Zhou, A;Blecher, JM;Wu, CJ;Lin, K;Shang, X;Jiang, S;Spring, DJ;Xia, Y;Chen, P;Shen, JP;Kopetz, S;DePinho, RA;
Cancer discovery 2023-09-28
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Extracellular vesicles are carriers of adiponectin with insulin-sensitizing and anti-inflammatory properties
Authors: Blandin, A;Amosse, J;Froger, J;Hilairet, G;Durcin, M;Fizanne, L;Ghesquière, V;Prieur, X;Chaigneau, J;Vergori, L;Dray, C;Pradère, JP;Blandin, S;Dupont, J;Ducluzeau, PH;Dubois, S;Boursier, J;Cariou, B;Le Lay, S;
Cell reports 2023-08-29
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Obesity promotes radioresistance through SERPINE1-mediated aggressiveness and DNA repair of triple-negative breast cancer
Authors: YH Su, YZ Wu, DK Ann, JL Chen, CY Kuo
Cell Death & Disease, 2023-01-21;14(1):53. 2023-01-21
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Fabricating 3-dimensional human brown adipose microtissues for transplantation studies
Authors: O Wang, L Han, H Lin, M Tian, S Zhang, B Duan, S Chung, C Zhang, X Lian, Y Wang, Y Lei
Bioactive materials, 2022-10-27;22(0):518-534. 2022-10-27
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Nicotinamide riboside kinase 1 protects against diet and age-induced pancreatic beta-cell failure
Authors: A Cercillieu, J Ratajczak, M Joffraud, JL Sanchez-Ga, G Jacot, A Zollinger, S Métairon, J Giroud-Ger, M Rumpler, E Ciarlo, M Valera-Alb, A Sambeat, C Canto
Molecular Metabolism, 2022-09-20;0(0):101605. 2022-09-20
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Inhibition of the Monocarboxylate Transporter 1 (MCT1) Promotes 3T3-L1 Adipocyte Proliferation and Enhances Insulin Sensitivity
Authors: T Bailey, A Nieto, P McDonald
International Journal of Molecular Sciences, 2022-02-08;23(3):. 2022-02-08
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Adipose MDM2 regulates systemic insulin sensitivity
Authors: P Hallenborg, BAH Jensen, E Fjære, RK Petersen, MS Belmaâti, SS Rasmussen, JP Gunnarsson, P Lauritzen, KKY Cheng, M Hermansson, SB Sonne, CS Ejsing, A Xu, I Kratchmaro, M Krüger, L Madsen, K Kristianse, B Blagoev
Scientific Reports, 2021-11-08;11(1):21839. 2021-11-08
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Crosstalk between Drp1 phosphorylation sites during mitochondrial remodeling and their impact on metabolic adaptation
Authors: M Valera-Alb, M Joffraud, J Miro-Blanc, J Capellades, A Junza, L Dayon, A Núñez Gali, JL Sanchez-Ga, A Valsesia, A Cercillieu, F Söllner, AG Ladurner, O Yanes, C Cantó
Cell Reports, 2021-08-24;36(8):109565. 2021-08-24
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Obesity alters ovarian folliculogenesis through disrupted angiogenesis from increased IL-10 production
Authors: PK Yang, CH Chou, CC Huang, WF Wen, HF Chen, CT Shun, HN Ho, MJ Chen
Molecular Metabolism, 2021-02-13;49(0):101189. 2021-02-13
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Pineapple Vinegar Regulates Obesity-Related Genes and Alters the Gut Microbiota in High-Fat Diet (HFD) C57BL/6 Obese Mice
Authors: NE Mohamad, SK Yeap, H Ky, NWC Liew, BK Beh, SY Boo, WY Ho, SA Sharifuddi, K Long, NB Alitheen
Evid Based Complement Alternat Med, 2020-09-23;2020(0):1257962. 2020-09-23
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Glucose transporter 10 modulates adipogenesis via an ascorbic acid-mediated pathway to protect mice against diet-induced metabolic dysregulation
Authors: CL Jiang, WP Jen, CY Tsao, LC Chang, CH Chen, YC Lee
PLoS Genet., 2020-05-26;16(5):e1008823. 2020-05-26
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Chemerin deficiency regulates adipogenesis is depot different through TIMP1
Authors: CL Huang, LL Xiao, M Xu, J Li, SF Li, CS Zhu, YL Lin, R He, X Li
Genes & diseases, 2020-04-09;8(5):698-708. 2020-04-09
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Functional expression of TRPM7 as a Ca2+ influx pathway in adipocytes
Authors: H Inoue, M Inazu, M Konishi, U Yokoyama
Physiol Rep, 2019-10-01;7(20):e14272. 2019-10-01
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Immune-Deficient Pfp/Rag2-/- Mice Featured Higher Adipose Tissue Mass and Liver Lipid Accumulation with Growing Age than Wildtype C57BL/6N Mice
Authors: S Winkler, M Hempel, MJ Hsu, M Gericke, H Kühne, S Brückner, S Erler, R Burkhardt, B Christ
Cells, 2019-07-25;8(8):. 2019-07-25
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Early postnatal soluble FGFR3 therapy prevents the atypical development of obesity in achondroplasia
Authors: C Saint-Laur, S Garcia, V Sarrazy, K Dumas, F Authier, S Sore, A Tran, P Gual, I Gennero, JP Salles, E Gouze
PLoS ONE, 2018-04-13;13(4):e0195876. 2018-04-13
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Insulin resistance causes inflammation in adipose tissue
Authors: M Shimobayas, V Albert, B Woelnerhan, IC Frei, D Weissenber, AC Meyer-Gers, N Clement, S Moes, M Colombi, JA Meier, MM Swierczyns, P Jenö, C Beglinger, R Peterli, MN Hall
J. Clin. Invest., 2018-03-12;0(0):. 2018-03-12
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Potential Hypoglycaemic and Antiobesity Effects of Senna italica Leaf Acetone Extract
Authors: RO Malematja, VP Bagla, I Njanje, V Mbazima, KW Poopedi, L Mampuru, MP Mokgotho
Evid Based Complement Alternat Med, 2018-02-19;2018(0):5101656. 2018-02-19
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Alterations in Adiposity and Glucose Homeostasis in Adult Gasp-1 Overexpressing Mice
Authors: L Périè, A Parenté, F Baraige, L Magnol, V Blanquet
Cell. Physiol. Biochem., 2017-12-08;44(5):1896-1911. 2017-12-08
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Adipose tissue supports normalization of macrophage and liver lipid handling in obesity reversal
Authors: M Vatarescu, S Bechor, Y Haim, T Pecht, T Tarnovsky, N Slutsky, O Nov, H Shapiro, A Shemesh, A Porgador, N Bashan, A Rudich
J. Endocrinol., 2017-03-30;0(0):. 2017-03-30
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Mfn2 is critical for brown adipose tissue thermogenic function
Authors: M Boutant, SS Kulkarni, M Joffraud, J Ratajczak, M Valera-Alb, R Combe, A Zorzano, C Cantó
EMBO J, 2017-03-27;0(0):. 2017-03-27
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Lipocalin-2 Promotes Pancreatic Ductal Adenocarcinoma by Regulating Inflammation in the Tumor Microenvironment
Authors: S Gomez-Chou, A Swidnicka-, N Badi, M Chavez-Tom, GB Lesinski, T Bekaii-Saa, MR Farren, TA Mace, C Schmidt, Y Liu, D Deng, RF Hwang, L Zhou, TT Moore, D Chatterjee, H Wang, X Leng, RB Arlinghaus, CD Logsdon, Z Cruz-Monse
Cancer Res, 2017-03-01;0(0):. 2017-03-01
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High-fat Diet Enhances Mammary Tumorigenesis and Pulmonary Metastasis and Alters Inflammatory and Angiogenic Profiles in MMTV-PyMT Mice
Authors: Sneha Sundaram
Anticancer Res, 2016-12-01;36(12):6279-6287. 2016-12-01
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Hsp90 blockers inhibit adipocyte differentiation and fat mass accumulation.
Authors: Desarzens S, Liao W, Mammi C, Caprio M, Faresse N
PLoS ONE, 2014-04-04;9(4):e94127. 2014-04-04
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Leucine-rich glioma inactivated 3 associates negatively with adiponectin.
Authors: Kim H, Kwon N, Baek K, Kim D, Yun H
Cytokine, 2013-03-30;62(2):206-9. 2013-03-30
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Efficacy and tolerability of a novel herbal formulation for weight management in obese subjects: a randomized double blind placebo controlled clinical study.
Authors: Sengupta K, Mishra A, Rao M, Sarma K, Krishnaraju A, Trimurtulu G
Lipids Health Dis, 2012-09-20;11(0):122. 2012-09-20
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Palmitate enhances the differentiation of mouse embryonic stem cells towards white adipocyte lineages.
Authors: Petrighi Polidori G, Lomax M, Docherty K
Mol Cell Endocrinol, 2012-04-05;361(1):40-50. 2012-04-05
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Obesity-related systemic factors promote an invasive phenotype in prostate cancer cells.
Authors: Price RS, Cavazos DA, De Angel RE, Hursting SD, Degraffenried LA
Prostate Cancer Prostatic Dis., 2012-02-14;15(2):135-43. 2012-02-14
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Age and sex mediated changes in epicardial fat adipokines.
Authors: Fei J, Cook C, Blough E, Santanam N
Atherosclerosis, 2010-07-07;212(2):488-94. 2010-07-07
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