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Porcine TNF-alpha Quantikine ELISA Kit

Catalog #: PTA00
85 Citations 2 Reviews Datasheet
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PTA00
Porcine TNF-alpha ELISA Cell Culture Supernate Standard Curve
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Citations (85)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Porcine TNF-alpha Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
5 pg/mL
Assay Range
23.4 - 1,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant porcine TNF-alpha
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Porcine TNF-alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure porcine TNF-alpha in cell culture supernates, serum, and plasma samples. It contains E. coli-expressed recombinant porcine TNF-alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately measure recombinant porcine TNF-alpha. Results obtained using natural porcine TNF-alpha show dose response curves that are parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural porcine TNF-alpha.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 79 260 646 88 279 679
Standard Deviation 4.9 9.2 27 8.8 24 44
CV% 6.2 3.5 4.2 10 8.6 6.5

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 104 358 880 106 348 866
Standard Deviation 7.2 13 36 9.7 32 72
CV% 6.9 3.6 4.1 9.2 9.2 8.3

Recovery

The recovery of porcine TNF-alpha spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 99 91-105
EDTA Plasma (n=6) 98 83-111
Heparin Plasma (n=4) 91 82-99
Serum (n=6) 105 91-120

Linearity

To assess the linearity of the assay, six samples containing and/or spiked with various concentrations of porcine TNF-alpha in each matrix were diluted with the appropriate Calibrator Diluent and then assayed.
Porcine TNF-alpha ELISA Linearity

Scientific Data

N/A Porcine TNF-alpha ELISA Cell Culture Supernate Standard Curve View Larger

Porcine TNF-alpha ELISA Cell Culture Supernate Standard Curve

N/A Porcine TNF-alpha ELISA Serum/Plasma Standard Curve View Larger

Porcine TNF-alpha ELISA Serum/Plasma Standard Curve

Product Datasheets

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Product Datasheet

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TNF-alpha

Tumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-α is produced by a wide variety of immune and epithelial cell types. Human TNF-α consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD). Within the ECD, human TNF-α shares 97% aa sequence identity with rhesus and 71% - 92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-α. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness.

Cleavage of membrane bound TNF-α by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-α. TNF-α trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers. TNF-α regulates lymphoid tissue development through control of apoptosis. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. TNF-α is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism.

Long Name:
Tumor Necrosis Factor alpha
Entrez Gene IDs:
7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 102139631 (Cynomolgus Monkey); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
Alternate Names:
APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 5 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Porcine TNF-alpha Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

85 Citations: Showing 1 - 10
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  1. Necroptosis contributes to the intestinal toxicity of deoxynivalenol and is mediated by methyltransferase SETDB1
    Authors: Zhou, B;Xiao, K;Guo, J;Xu, Q;Xu, Q;Lv, Q;Zhu, H;Zhao, J;Liu, Y;
    Journal of hazardous materials
    Species: Porcine
    Sample Types: Cell Culture Supernates
  2. Supplementation of vitamin E or a botanical extract as antioxidants to improve growth performance and health of growing pigs housed under thermoneutral or heat-stressed conditions
    Authors: Silva-Guillen, YV;Arellano, C;Wiegert, J;Boyd, RD;Martínez, GE;van Heugten, E;
    Journal of animal science and biotechnology
    Species: Porcine
    Sample Types: Tissue Homogenates
  3. Analysis of the Model of Atherosclerosis Formation in Pig Hearts as a Result of Impaired Activity of DNA Repair Enzymes
    Authors: Paslawski, R;Kowalczyk, P;Paslawska, U;Wi?niewski, J;Dzi?giel, P;Janiszewski, A;Kiczak, L;Zacharski, M;Gawdzik, B;Kramkowski, K;Szuba, A;
    International journal of molecular sciences
    Species: Porcine
    Sample Types: Serum
  4. Early weaning and biological sex shape long-term immune and metabolic responses in pigs
    Authors: Fardisi, M;Thelen, K;Groenendal, A;Rajput, M;Sebastian, K;Contreras, GA;Moeser, AJ;
    Scientific reports
    Species: Porcine
    Sample Types: Plasma
  5. Porcine circovirus type 2 infection inhibits macrophage M1 polarization induced by other pathogens via viral capsid protein and host gC1qR protein
    Authors: Yang, X;Du, Q;Wang, X;Shi, J;Wang, T;Li, P;Zhong, J;Tong, D;Huang, Y;
    Veterinary microbiology
    Species: Porcine
    Sample Types: Cell Culture Supernates
  6. Efficacy of Feed Additive Containing Bentonite and Enzymatically Hydrolyzed Yeast on Intestinal Health and Growth of Newly Weaned Pigs under Chronic Dietary Challenges of Fumonisin and Aflatoxin
    Authors: Deng, Z;Jang, KB;Jalukar, S;Du, X;Kim, SW;
    Toxins
    Species: Porcine
    Sample Types: Tissue Homogenates
  7. Aqueous cannabidiol ?-cyclodextrin complexed polymeric micelle nasal spray to attenuate in vitro and ex vivo SARS-CoV-2-induced cytokine storms
    Authors: Changsan, N;Sawatdee, S;Suedee, R;Chunhachaichana, C;Srichana, T;
    International journal of pharmaceutics
    Species: Porcine
    Sample Types: Tissue Homogenates
  8. Regenerative Effect of Mesenchymal Stem Cell on Cartilage Damage in a Porcine Model
    Authors: Lin, S;Panthi, S;Hsuuw, Y;Chen, S;Huang, M;Sieber, M;Hsuuw, Y;
    Biomedicines
    Species: Porcine
    Sample Types: Serum
  9. Phytogenic feed additives alleviate pathogenic Escherichia coli-induced intestinal damage through improving barrier integrity and inhibiting inflammation in weaned pigs
    Authors: SY Chang, MH Song, JH Lee, HJ Oh, YJ Kim, JW An, YB Go, DC Song, HA Cho, SY Cho, DJ Kim, MS Kim, HB Kim, JH Cho
    Journal of animal science and biotechnology, 2022-09-02;13(1):107.
    Species: Porcine
    Sample Types: Serum
  10. Supplementation With Spirulina Reduces Infarct Size and Ameliorates Cardiac Function in a Pig Model of STEMI
    Authors: G Vilahur, P Sutelman, S Ben-Aicha, G Mendieta, M Radiké, L Schoch, L Casaní, M Borrell-Pa, T Padro, L Badimon
    Frontiers in Pharmacology, 2022-05-03;13(0):891801.
    Species: Porcine
    Sample Types: Plasma
  11. Levosimendan Ameliorates Cardiopulmonary Function but Not Inflammatory Response in a Dual Model of Experimental ARDS
    Authors: R Rissel, M Gosling, J Kamuf, M Renz, R Ruemmler, A Ziebart, EK Hartmann
    Biomedicines, 2022-04-29;10(5):.
    Species: Porcine
    Sample Types: Plasma
  12. Impacts of weaning weights and mycotoxin challenges on jejunal mucosa-associated microbiota, intestinal and systemic health, and growth performance of nursery pigs
    Authors: DM Holanda, SW Kim
    Journal of animal science and biotechnology, 2022-04-13;13(1):43.
    Species: Porcine
    Sample Types: Complex Sample Type
  13. Assessment of the Effects of the Synbiotic Combination of Bifidobacterium longum subsp. infantis CECT 7210 and Oligofructose-Enriched Inulin Against Digestive Bacterial Infections in a Piglet Model
    Authors: A Rodríguez-, L Castillejo, P López-Colo, G Cifuentes-, JA Moreno-Muñ, SM Martín-Orú
    Frontiers in Microbiology, 2022-03-08;13(0):831737.
    Species: Porcine
    Sample Types: Serum
  14. Hemosiderin Accumulation in Liver Decreases Iron Availability in Tachycardia-Induced Porcine Congestive Heart Failure Model
    Authors: M Kasztura, L Kiczak, U Pas?awska, J Bania, A Janiszewsk, A Tomaszek, M Zacharski, A Noszczyk-N, R Pas?awski, A Tabi?, P Kuropka, P Dzi?giel, P Ponikowski
    International Journal of Molecular Sciences, 2022-01-18;23(3):.
    Species: Porcine
    Sample Types: Serum
  15. Influence of rosuvastatin treatment on cerebral inflammation and nitro-oxidative stress in experimental lung injury in pigs
    Authors: J Kamuf, A Garcia Bar, A Ziebart, R Ruemmler, J Schwab, M Dib, A Daiber, SC Thal, EK Hartmann
    BMC anesthesiology, 2021-09-13;21(1):224.
    Species: Porcine
    Sample Types: Serum
  16. IFN-gamma Licensing Does Not Enhance the Reduced Immunomodulatory Potential and Migratory Ability of Differentiation-Induced Porcine Bone Marrow-Derived Mesenchymal Stem Cells in an In Vitro Xenogeneic Application
    Authors: HJ Lee, HD Kim, CH Jo, EY Bok, SB Kim, SL Lee, M Jang, SG Bae, SH Yun, SJ Kim, GJ Rho, WJ Lee
    BioMed Research International, 2021-09-04;2021(0):4604856.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  17. Phytobiotics with Adsorbent to Mitigate Toxicity of Multiple Mycotoxins on Health and Growth of Pigs
    Authors: DM Holanda, YI Kim, W Parnsen, SW Kim
    Toxins, 2021-06-26;13(7):.
    Species: Porcine
    Sample Types: Serum
  18. Epigenetic Modulation of TLR4 Expression by Sulforaphane Increases Anti-Inflammatory Capacity in Porcine Monocyte-Derived Dendritic Cells
    Authors: X Qu, C Neuhoff, MU Cinar, M Pröll, E Tholen, D Tesfaye, M Hölker, K Schellande, MJ Uddin
    Biology, 2021-05-31;10(6):.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  19. Effects of the Administration of Bifidobacterium longum subsp. infantis CECT 7210 and Lactobacillus rhamnosus HN001 and Their Synbiotic Combination With Galacto-Oligosaccharides Against Enterotoxigenic Escherichia coli F4 in an Early Weaned Piglet Model
    Authors: A Rodríguez-, L Castillejo, P López-Colo, G Cifuentes-, M Rodríguez-, JA Moreno-Muñ, D Luise, P Trevisi, SM Martín-Orú
    Frontiers in Microbiology, 2021-04-16;12(0):642549.
    Species: Porcine
    Sample Types: Serum
  20. SOFA Score, Hemodynamics and Body Temperature Allow Early Discrimination between Porcine Peritonitis-Induced Sepsis and Peritonitis-Induced Septic Shock
    Authors: M Al-Obeidal, D Jarkovská, L Valešová, J Horák, J Jedli?ka, L Nalos, J Chvojka, J Švíglerová, J Kuncová, J Beneš, M Mat?jovi?, M Štengl
    Journal of personalized medicine, 2021-02-28;11(3):.
    Species: Porcine
    Sample Types: Plasma
  21. Hemodynamic effects of high frequency oscillatory ventilation with volume guarantee in a piglet model of respiratory distress syndrome
    Authors: J Bhogal, AL Solevåg, M O'Reilly, TF Lee, C Joynt, LK Hornberger, GM Schmölzer, PY Cheung
    PLoS ONE, 2021-02-16;16(2):e0246996.
    Species: Porcine
    Sample Types: Tissue Homogenates
  22. Experimental lung injury induces cerebral cytokine mRNA production in pigs
    Authors: J Kamuf, A Garcia Bar, A Ziebart, K Frauenknec, K Folkert, J Schwab, R Ruemmler, M Renz, D Cana, SC Thal, EK Hartmann
    PeerJ, 2020-12-09;8(0):e10471.
    Species: Porcine
    Sample Types: Whole Blood
  23. Thymol modulates the endocannabinoid system and gut chemosensing of weaning pigs
    Authors: A Toschi, B Tugnoli, B Rossi, A Piva, E Grilli
    BMC Vet. Res., 2020-08-12;16(1):289.
    Species: Porcine
    Sample Types: Tissue Homogenates, Tissue Lysates
  24. Investigation of the Efficacy of a Postbiotic Yeast Cell Wall-Based Blend on Newly-Weaned Pigs under a Dietary Challenge of Multiple Mycotoxins with Emphasis on Deoxynivalenol
    Authors: DM Holanda, A Yiannikour, SW Kim
    Toxins (Basel), 2020-08-06;12(8):.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  25. Hypochlorite-induced porcine model of peritoneal fibrosis through the activation of IL1&beta-CX3CL1-TGF&beta1 signal axis
    Authors: YT Hsu, CH Wu, CY Chao, YS Wei, YC Chang, YT Chen, SL Lin, SY Tsai, YJ Lee, PS Tsai
    Sci Rep, 2020-07-13;10(1):11496.
    Species: Porcine
    Sample Types: Serum
  26. Effects of sustained inflation pressure during�neonatal cardiopulmonary resuscitation of asphyxiated piglets
    Authors: GH Shim, SY Kim, PY Cheung, TF Lee, M O'Reilly, GM Schmölzer
    PLoS ONE, 2020-06-23;15(6):e0228693.
    Species: Porcine
    Sample Types: Tissue Homogenates
  27. Efficacy of Mycotoxin Detoxifiers on Health and Growth of Newly-Weaned Pigs under Chronic Dietary Challenge of Deoxynivalenol
    Authors: DM Holanda, SW Kim
    Toxins (Basel), 2020-05-09;12(5):.
    Species: Porcine
    Sample Types: Tissue Homogenates
  28. Low Protein-High Carbohydrate Diets Alter Energy Balance, Gut Microbiota Composition and Blood Metabolomics Profile in Young Pigs
    Authors: S Spring, H Premathila, U DeSilva, C Shili, S Carter, A Pezeshki
    Sci Rep, 2020-02-24;10(1):3318.
    Species: Porcine
    Sample Types: Serum
  29. Efficacy of medium-chain fatty acid salts distilled from coconut oil against two enteric pathogen challenges in weanling piglets
    Authors: P López-Colo, L Castillejo, A Rodríguez-, M Puyalto, JJ Mallo, SM Martín-Orú
    J Anim Sci Biotechnol, 2019-11-09;10(0):89.
    Species: Porcine
    Sample Types: Serum
  30. Sepsis causes right ventricular myocardial inflammation independent of pulmonary hypertension in a porcine sepsis model
    Authors: SE Pischke, S Hestenes, HT Johansen, H Fure, JF Bugge, A Espinoza, H Skulstad, T Edvardsen, E Fosse, TE Mollnes, PS Halvorsen, EW Nielsen
    PLoS ONE, 2019-06-27;14(6):e0218624.
    Species: Porcine
    Sample Types: Plasma
  31. Toll-like receptor 5-mediated IL-17C expression in intestinal epithelial cells enhances epithelial host defense against F4+ ETEC infection
    Authors: Y Luo, J Xu, C Zhang, C Jiang, Y Ma, H He, Y Wu, B Devriendt, E Cox, H Zhang
    Vet. Res., 2019-06-20;50(1):48.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  32. Dietary supplementation of xylanase and protease on growth performance, digesta viscosity, nutrient digestibility, immune and oxidative stress status, and gut health of newly weaned pigs
    Authors: ME Duarte, FX Zhou, WM Dutra, SW Kim
    Anim Nutr, 2019-05-11;5(4):351-358.
    Species: Porcine
    Sample Types: Serum
  33. I-FABP, Pig-MAP and TNF-? as biomarkers for monitoring gut-wall integrity in front of Salmonella Typhimurium and ETEC K88 infection in a weaned piglet model
    Authors: P López-Colo, K Yu, E Barba-Vida, Y Saco, SM Martín-Orú, L Castillejo, D Solà-Oriol, A Bassols
    Res. Vet. Sci., 2019-05-07;124(0):426-432.
    Species: Porcine
    Sample Types: Serum
  34. Nonmuscle Myosin Heavy Chain IIA Recognizes Sialic Acids on Sialylated RNA Viruses To Suppress Proinflammatory Responses via the DAP12-Syk Pathway
    Authors: Y Liu, R Li, XX Chen, Y Zhi, R Deng, EM Zhou, S Qiao, G Zhang
    MBio, 2019-05-07;10(3):.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  35. Modulation of the somatotropic axis, adiponectin and cytokine secretion during highly pathogenic porcine reproductive and respiratory syndrome virus type 1 (HP-PRRSV-1) infection
    Authors: R Saleri, V Cavalli, L Ferrari, G Ogno, E Canelli, P Martelli, P Borghetti
    Res. Vet. Sci., 2019-04-11;124(0):263-269.
    Species: Porcine
    Sample Types: Plasma
  36. PO2 oscillations induce lung injury and inflammation
    Authors: S Boehme, EK Hartmann, T Tripp, SC Thal, M David, D Abraham, JE Baumgardne, K Markstalle, KU Klein
    Crit Care, 2019-03-27;23(1):102.
    Species: Porcine
    Sample Types: Plasma
  37. Medium-Chain Triglycerides Attenuate Liver Injury in Lipopolysaccharide-Challenged Pigs by Inhibiting Necroptotic and Inflammatory Signaling Pathways
    Authors: L Zhang, X Wang, S Chen, S Wang, Z Tu, G Zhang, H Zhu, X Li, J Xiong, Y Liu
    Int J Mol Sci, 2018-11-21;19(11):.
    Species: Porcine
    Sample Types: Serum
  38. Neuroimmunological Implications of Subclinical Lipopolysaccharide from Salmonella Enteritidis
    Authors: A Miko?ajczy, D Z?otkowska
    Int J Mol Sci, 2018-10-22;19(10):.
    Species: Porcine
    Sample Types: Serum
  39. Glutamate alleviates intestinal injury, maintains mTOR and suppresses TLR4 and NOD signaling pathways in weanling pigs challenged with lipopolysaccharide
    Authors: Q Qin, X Xu, X Wang, H Wu, H Zhu, Y Hou, B Dai, X Liu, Y Liu
    Sci Rep, 2018-10-11;8(1):15124.
    Species: Porcine
    Sample Types: Plasma
  40. Blood pressure targeting by partial REBOA is possible in severe hemorrhagic shock in pigs and produces less circulatory, metabolic and inflammatory sequelae than total REBOA
    Authors: M Sadeghi, TM Hörer, D Forsman, EM Dogan, K Jansson, C Kindler, P Skoog, KF Nilsson
    Injury, 2018-09-29;0(0):.
    Species: Porcine
    Sample Types: Serum
  41. Pulmonary Effects of Remote Ischemic Preconditioning in a Porcine Model of Ventilation-induced Lung Injury
    Authors: A Bergmann, T Schilling, G Hedenstier, K Ahlgren, A Larsson, M Kretzschma, A Kozian, T Hachenberg
    Respir Physiol Neurobiol, 2018-08-31;0(0):.
    Species: Porcine
    Sample Types: Tissue Homogenates
  42. Inflammatory markers before and after farrowing in healthy sows and in sows affected with postpartum dysgalactia syndrome
    Authors: M Kaiser, M Jacobson, PH Andersen, P Bækbo, JJ Cerón, J Dahl, D Escribano, S Jacobsen
    BMC Vet. Res., 2018-03-12;14(1):83.
    Species: Porcine
    Sample Types: Serum
  43. Medium-chain TAG improve intestinal integrity by suppressing toll-like receptor 4, nucleotide-binding oligomerisation domain proteins and necroptosis signalling in weanling piglets challenged with lipopolysaccharide
    Authors: X Xu, S Chen, H Wang, Z Tu, S Wang, X Wang, H Zhu, C Wang, J Zhu, Y Liu
    Br. J. Nutr., 2018-03-06;0(0):1-10.
    Species: Porcine
    Sample Types: Plasma
  44. Sodium lactate improves renal microvascular thrombosis compared to sodium bicarbonate and 0.9% NaCl in a porcine model of endotoxic shock: an experimental randomized open label controlled study
    Authors: T Duburcq, A Durand, A Tournoys, V Gnemmi, V Gmyr, F Pattou, M Jourdain, F Tamion, E Besnier, S Préau, E Parmentier, D Mathieu, J Poissy, R Favory
    Ann Intensive Care, 2018-02-14;8(1):24.
    Species: Porcine
    Sample Types: Serum
  45. Influence of zinc supplementation on immune parameters in weaned pigs
    Authors: V Kloubert, K Blaabjerg, TS Dalgaard, HD Poulsen, L Rink, I Wessels
    J Trace Elem Med Biol, 2018-01-31;0(0):.
    Species: Porcine
    Sample Types: Whole Blood
    Applications: ELISA Capture
  46. Beneficial effects of inhaled nitric oxide with intravenous steroid in an ischemia-reperfusion model involving aortic clamping
    Authors: W Gozdzik, S Zielinski, M Zielinska, K Ratajczak, P Skrzypczak, S Rodziewicz, A Kübler, K Löfström, P Dziegiel, M Olbromski, B Adamik, S Ryniak, P Harbut, J Albert, C Frostell
    Int J Immunopathol Pharmacol, 2018-01-01;32(0):3946320177514.
    Species: Porcine
    Sample Types: Plasma
  47. Butyrate modifies intestinal barrier function in IPEC-J2 cells through a selective upregulation of tight junction proteins and activation of the Akt signaling pathway
    Authors: H Yan, KM Ajuwon
    PLoS ONE, 2017-06-27;12(6):e0179586.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  48. Altered Cytokine Expression and Barrier Properties after In Vitro Infection of Porcine Epithelial Cells with Enterotoxigenic Escherichia coli and Probiotic Enterococcus faecium
    Authors: M Kern, D Günzel, JR Aschenbach, K Tedin, A Bondzio, U Lodemann
    Mediators Inflamm., 2017-05-14;2017(0):2748192.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  49. Septic porcine blood does not further activate coagulation during in vitro membrane oxygenation
    Authors: Rolf Rossaint
    Eur J Cardiothorac Surg, 2017-03-01;0(0):.
    Species: Porcine
    Sample Types: Plasma
  50. Inhibition of CD34+ cell migration by matrix metalloproteinase-2 during acute myocardial ischemia, counteracted by ischemic preconditioning
    Authors: D Lukovic, K Zlabinger, A Gugerell, A Spannbauer, N Pavo, L Mandic, DT Weidenauer, S Kastl, C Kaun, A Posa, I Sabdyushev, J Winkler, M Gyöngyösi
    F1000Res, 2016-11-22;5(0):2739.
    Species: Porcine
    Sample Types: Serum
  51. An Immunomodulatory Device Improves Insulin Resistance in Obese Porcine Model of Metabolic Syndrome
    Authors: Angela J Westover
    J Diabetes Res, 2016-10-13;2016(0):3486727.
    Species: Porcine
    Sample Types: Plasma
  52. Sulforaphane epigenetically regulates innate immune responses of porcine monocyte-derived dendritic cells induced with lipopolysaccharide.
    Authors: Qu X, Proll M, Neuhoff C, Zhang R, Cinar M, Hossain M, Tesfaye D, Grosse-Brinkhaus C, Salilew-Wondim D, Tholen E, Looft C, Holker M, Schellander K, Uddin M
    PLoS ONE, 2015-03-20;10(3):e0121574.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  53. Inhalation therapy with the synthetic TIP-like peptide AP318 attenuates pulmonary inflammation in a porcine sepsis model.
    Authors: Hartmann E, Ziebart A, Thomas R, Liu T, Schad A, Tews M, Moosmann B, Kamuf J, Duenges B, Thal S, David M
    BMC Pulm Med, 2015-02-07;15(0):7.
    Species: Porcine
    Sample Types: Plasma
  54. Soluble T cell immunoglobulin mucin domain 3 is shed from CD8+ T cells by the sheddase ADAM10, is increased in plasma during untreated HIV infection, and correlates with HIV disease progression.
    Authors: Clayton K, Douglas-Vail M, Nur-ur Rahman A, Medcalf K, Xie I, Chew G, Tandon R, Lanteri M, Norris P, Deeks S, Ndhlovu L, Ostrowski M
    J Virol, 2015-01-21;89(7):3723-36.
    Species: Porcine
    Sample Types: Plasma
  55. Low tidal volume pressure support versus controlled ventilation in early experimental sepsis in pigs.
    Authors: Ziebart A, Hartmann E, Thomas R, Liu T, Duenges B, Schad A, Bodenstein M, Thal S, David M
    Respir Res, 2014-09-06;15(0):101.
    Species: Porcine
    Sample Types: Plasma
  56. Risks Associated with High-Dose Lactobacillus rhamnosus in an Escherichia coli Model of Piglet Diarrhoea: Intestinal Microbiota and Immune Imbalances.
    Authors: Li XQ, Zhu YH, Zhang HF, Yue Y, Cai ZX, Lu QP, Zhang L, Weng XG, Zhang FJ, Zhou D, Yang JC, Wang JF
    PLoS ONE, 2012-07-27;7(7):e40666.
    Species: Porcine
    Sample Types: Serum
  57. Ex vivo adenoviral vector gene delivery results in decreased vector-associated inflammation pre- and post-lung transplantation in the pig.
    Authors: Yeung JC, Wagnetz D, Cypel M, Rubacha M, Koike T, Chun YM, Hu J, Waddell TK, Hwang DM, Liu M, Keshavjee S
    Mol. Ther., 2012-03-27;20(6):1204-11.
    Species: Porcine
    Sample Types: Plasma
  58. Efficacy of linezolid compared to vancomycin in an experimental model of pneumonia induced by methicillin-resistant Staphylococcus aureus in ventilated pigs.
    Authors: Martinez-Olondris P, Rigol M, Soy D, Guerrero L, Agusti C, Quera MA, Li Bassi G, Esperatti M, Luque N, Liapikou M, Filella X, Marco F, de la Bellacasa JP, Torres A
    Crit. Care Med., 2012-01-01;40(1):162-8.
    Species: Porcine
    Sample Types: Serum
  59. Ornithodoros moubata complement inhibitor is an equally effective C5 inhibitor in pigs and humans.
    Authors: Barratt-Due A, Thorgersen EB, Lindstad JK, Pharo A, Lissina O, Lambris JD, Nunn MA, Mollnes TE
    J. Immunol., 2011-09-30;187(9):4913-9.
    Species: Porcine
    Sample Types: Plasma
  60. Dissecting the effects of lipopolysaccharides from nonlipopolysaccharide molecules in experimental porcine meningococcal sepsis.
    Authors: Hellerud BC, Nielsen EW, Thorgersen EB, Lindstad JK, Pharo A, Tonnessen TI, Castellheim A, Mollnes TE, Brandtzaeg P
    Crit. Care Med., 2010-06-01;38(6):1467-74.
    Species: Porcine
    Sample Types: Plasma
  61. Plasma concentrations of inflammatory cytokines rise rapidly during ECMO-related SIRS due to the release of preformed stores in the intestine.
    Authors: McILwain RB, Timpa JG, Kurundkar AR
    Lab. Invest., 2009-11-09;90(1):128-39.
    Species: Porcine
    Sample Types: Plasma
  62. Cardiac Function and the Proinflammatory Cytokine Response After Recovery From Cardiac Arrest in Swine.
    Authors: Niemann JT, Rosborough JP, Youngquist S, Shah AP, Lewis RJ, Phan QT, Filler SG
    J. Interferon Cytokine Res., 2009-11-01;0(0):.
    Species: Porcine
    Sample Types: Plasma
  63. CD14 inhibition efficiently attenuates early inflammatory and hemostatic responses in Escherichia coli sepsis in pigs.
    Authors: Thorgersen EB, Hellerud BC, Nielsen EW, Barratt-Due A, Fure H, Lindstad JK, Pharo A, Fosse E, Tonnessen TI, Johansen HT, Castellheim A, Mollnes TE
    FASEB J., 2009-10-19;24(3):712-22.
    Species: Porcine
    Sample Types: Plasma
  64. Autologous mesenchymal stem cells produce reverse remodelling in chronic ischaemic cardiomyopathy.
    Authors: Schuleri KH, Feigenbaum GS, Centola M, Weiss ES, Zimmet JM, Turney J, Kellner J, Zviman MM, Hatzistergos KE, Detrick B, Conte JV, McNiece I, Steenbergen C, Lardo AC, Hare JM
    Eur. Heart J., 2009-07-08;30(22):2722-32.
    Species: Porcine
    Sample Types: Serum
  65. Metabolic activity of the enteric microbiota influences the fatty acid composition of murine and porcine liver and adipose tissues.
    Authors: Wall R, Ross RP, Shanahan F, O'Mahony L, O'Mahony C, Coakley M, Hart O, Lawlor P, Quigley EM, Kiely B, Fitzgerald GF, Stanton C
    Am. J. Clin. Nutr., 2009-04-08;89(5):1393-401.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  66. Effect of linezolid compared with glycopeptides in methicillin-resistant Staphylococcus aureus severe pneumonia in piglets.
    Authors: Luna CM, Bruno DA, Garcia-Morato J, Mann KC, Risso Patron J, Sagardia J, Absi R, Garcia Bottino M, Marchetti D, Famiglietti A, Baleztena M, Biancolini C
    Chest, 2009-01-13;135(6):1564-71.
    Species: Porcine
    Sample Types: Serum
  67. Inhibition of complement and CD14 attenuates the Escherichia coli-induced inflammatory response in porcine whole blood.
    Authors: Thorgersen EB, Pharo A, Haverson K, Axelsen AK, Gaustad P, Kotwal GJ, Sfyroera G, Mollnes TE
    Infect. Immun., 2008-12-01;77(2):725-32.
    Species: Porcine
    Sample Types: Whole Blood
  68. Inducible nitric oxide synthase expression and cardiomyocyte dysfunction during sustained moderate ischemia in pigs.
    Authors: Heinzel FR, Gres P, Boengler K, Duschin A, Konietzka I, Rassaf T, Snedovskaya J, Meyer S, Skyschally A, Kelm M, Heusch G, Schulz R
    Circ. Res., 2008-09-25;103(10):1120-7.
    Species: Porcine
    Sample Types: Cell Lysates
  69. Blood concentrations of the cytokines IL-1beta, IL-6, IL-10, TNF-alpha and IFN-gamma during experimentally induced swine dysentery.
    Authors: Kruse R, Essen-Gustavsson B, Fossum C, Jensen-Waern M
    Acta Vet. Scand., 2008-08-12;50(0):32.
    Species: Porcine
    Sample Types: Serum
  70. Effects of glucocorticoids in ventilated piglets with severe pneumonia.
    Authors: Sibila O, Luna CM, Agusti C, Baquero S, Gando S, Patron JR, Morato JG, Absi R, Bassi N, Torres A
    Eur. Respir. J., 2008-05-28;32(4):1037-46.
    Species: Porcine
    Sample Types: Serum
  71. Cytokine secretion depends on Galalpha(1,3)Gal expression in a pig-to-human whole blood model.
    Authors: Saethre M, Schneider MK, Lambris JD, Magotti P, Haraldsen G, Seebach JD, Mollnes TE
    J. Immunol., 2008-05-01;180(9):6346-53.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  72. Protective effect of Sivelestat in a porcine hepatectomy model prepared using an intermittent Pringle method.
    Authors: Shimoda M, Iwasaki Y, Okada T, Sawada T, Kubota K
    Eur. J. Pharmacol., 2008-02-29;587(1):248-52.
    Species: Porcine
    Sample Types: Serum
  73. Activated protein C downregulates p38 mitogen-activated protein kinase and improves clinical parameters in an in-vivo model of septic shock.
    Authors: Nold MF, Nold-Petry CA, Fischer D, Richter B, Blaheta R, Pfeilschifter J, Muhl H, Schranz D, Veldman A
    Thromb. Haemost., 2007-11-01;98(5):1118-26.
    Species: Porcine
    Sample Types: Plasma
  74. Involvement of both tumor necrosis factor-alpha-induced necrosis and p53-mediated caspase-dependent apoptosis in nephrotoxicity of cisplatin.
    Authors: Yano T, Itoh Y, Matsuo M, Kawashiri T, Egashira N, Oishi R
    Apoptosis, 2007-10-01;12(10):1901-9.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  75. Experimental Pseudomonas aeruginosa pneumonia: evaluation of the associated inflammatory response.
    Authors: Sibila O, Agusti C, Torres A, Baquero S, Gando S, Patron JR, Morato JG, Goffredo DH, Bassi N, Luna CM
    Eur. Respir. J., 2007-09-05;30(6):1167-72.
    Species: Porcine
    Sample Types: Serum
  76. Experimental severe Pseudomonas aeruginosa pneumonia and antibiotic therapy in piglets receiving mechanical ventilation.
    Authors: Luna CM, Baquero S, Gando S, Patron JR, Morato JG, Sibila O, Absi R, Famiglietti A, Vay CA, Von Stecher F, Agusti C, Torres A
    Chest, 2007-08-01;132(2):523-31.
    Species: Porcine
    Sample Types: Serum
  77. The fibrin-derived peptide Bbeta15-42 is cardioprotective in a pig model of myocardial ischemia-reperfusion injury.
    Authors: Roesner JP, Petzelbauer P, Koch A, Mersmann J, Zacharowski PA, Boehm O, Reingruber S, Pasteiner W, Mascher D, Wolzt M, Barthuber C, Noldge-Schomburg GE, Scheeren TW, Zacharowski K
    Crit. Care Med., 2007-07-01;35(7):1730-5.
    Species: Porcine
    Sample Types: Plasma
  78. Bidirectional role of tumor necrosis factor-alpha in coronary microembolization: progressive contractile dysfunction versus delayed protection against infarction.
    Authors: Skyschally A, Gres P, Hoffmann S, Haude M, Erbel R, Schulz R, Heusch G
    Circ. Res., 2006-12-14;100(1):140-6.
    Species: Porcine
    Sample Types: Tissue Homogenates
  79. Dietary fatty acid composition rather than vitamin E supplementation influence ex vivo cytokine and eicosanoid response of porcine alveolar macrophages.
    Authors: Moller S, Lauridsen C
    Cytokine, 2006-08-17;35(1):6-12.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  80. Role of oxidative stress in remodeling of the myocardial microcirculation in hypertension.
    Authors: Zhu XY, Daghini E, Chade AR, Rodriguez-Porcel M, Napoli C, Lerman A, Lerman LO
    Arterioscler. Thromb. Vasc. Biol., 2006-05-18;26(8):1746-52.
    Species: Porcine
    Sample Types: Plasma
  81. Proangiogenic effects of protease-activated receptor 2 are tumor necrosis factor-alpha and consecutively Tie2 dependent.
    Authors: Zhu T, Sennlaub F, Beauchamp MH, Fan L, Joyal JS, Checchin D, Nim S, Lachapelle P, Sirinyan M, Hou X, Bossolasco M, Rivard GE, Heveker N, Chemtob S
    Arterioscler. Thromb. Vasc. Biol., 2006-01-26;26(4):744-50.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  82. Conjugated linoleic acid attenuates the production and gene expression of proinflammatory cytokines in weaned pigs challenged with lipopolysaccharide.
    Authors: Changhua L, Jindong Y, Defa L, Lidan Z, Shiyan Q, Jianjun X
    J. Nutr., 2005-02-01;135(2):239-44.
    Species: Porcine
    Sample Types: Serum
  83. Deciphering the involvement of innate immune factors in the development of the host response to PRRSV vaccination.
    Authors: Royaee AR, Husmann RJ, Dawson HD, Calzada-Nova G, Schnitzlein WM, Zuckermann FA, Lunney JK
    Vet. Immunol. Immunopathol., 2004-12-08;102(3):199-216.
    Species: Porcine
    Sample Types: Whole Cells
  84. The effect of warm humidified CO2 on the dissipation of residual gas following laparoscopy in piglets.
    Authors: Glew PA, Campher MJ, Pearson K, Schofield JC, Davey AK
    J Am Assoc Gynecol Laparosc, 2004-05-01;11(2):204-10.
    Species: Porcine
    Sample Types: Serum
  85. Extracellular ubiquitin inhibits the TNF-alpha response to endotoxin in peripheral blood mononuclear cells and regulates endotoxin hyporesponsiveness in critical illness.
    Authors: Majetschak M, Krehmeier U, Bardenheuer M, Denz C, Quintel M, Voggenreiter G, Obertacke U
    Blood, 2002-10-24;101(5):1882-90.
    Species: Porcine
    Sample Types: Plasma

FAQs

  1. Assay Diluent RD1-63 contains visible precipitate. Is this acceptable to use?

    • A small amount of precipitate is expected and acceptable in Assay Diluent RD1-63. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.

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