Porcine Luminex® Discovery Assay

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Porcine Luminex Discovery Panel Standard Curves
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Product Details
Citations (3)
FAQs
Supplemental Products
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Porcine Luminex® Discovery Assay Summary

Assay Type Magnetic bead-based multiplex assay for the Luminex® platform
Format 1 x 96-well microplate and Magnetic antibody-coated beads
Species Porcine
Analytes Detected Please see analyte list in assay customization tool below.
Performance Validation Luminex Assays are validated for use with cell culture supernatants, plasma and serum. All Luminex assays are tested for sensitivity, intra-assay precision, inter-assay precision and to ensure assay linearity for validated sample types. Antibody pairs are selected and tested to confirm the parallel detection of natural and recombinant standard protein and to ensure the accurate determination of target analytes within biological samples. Assays for each target analyte are screened against all target analytes to confirm low antibody cross-reactivity.

Make your selections below to configure your assay

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Assays

Below are kits that are available based on your selections. These selections may be further customized before adding to the cart or emailing the configuration to your purchasing department or local distributor to place your order. Selected analytes may be included in more than one High Performance Assay. All assay options are displayed.

If you run into bead region incompatibilities, send your Luminex code to our team and find out more.

How would you like these analytes mixed?

Premixed
User Mixed
Please select at least one mixing option to see the kits.

Would you like to split based on dilution?

Do not split
Split based on serum
Split based on plasma
Split based on cell culture supernates

Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Ordering Tool. We also offer Custom Luminex Services

Product Features

Magnetic Luminex Assays are the most flexible bead-based multiplex assays that we offer. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples.

  • Simultaneously profile up to 50 analytes of your choice in one sample
  • Choose from a wide menu of analytes, including many unique analytes
  • Requires a small sample volume (<50 µL)
  • Run your assay in just 3-4 hours
  • Mass-calibrated standards for consistent results with every new lot of material
  • Enough reagents for one 96-well plate
  • Quick turn-around time: Kits are typically shipped within 5-10 days of ordering.*

Please allow additional time for international orders.

Kit Components
  • Premixed cocktail of antibody-coated Magnetic beads
  • Premixed cocktail of biotinylated detection antibody
  • Standard Cocktail(s)
  • Bead Diluent
  • Biotin Antibody Diluent
  • Standard/Sample Diluent
  • Wash Buffer
  • Streptavidin-PE
  • One flat-bottom 96-well Microplate
  • Foil Plate Sealers (4)
  • Mixing Bottle
  • Certificate of Analysis**

**A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.

Background

Pig animal models have an established track record of success in scientific research across a range of study areas, including cardiovascular, clinical work, pharmacology, toxicology, aging, and more recently in stem cell therapies. Porcine models share many similarities to human physiology, anatomy, and mechanisms of human disease, making them a unique valuable experimental model to test new disease therapies, devices and safety and efficacy of new drugs preceding human trials. In addition to transplantation research, a wide range of viral, bacterial, cancer, and inflammatory disease translational research has leveraged the pig’s close homology to humans.  At the same time, some “minipig” breeds are providing unique value in longer-term pre-clinical studies due to their ability to age without growing too large, enabling researchers to complete multi-generational studies in comparatively compact timeframes. Emerging human disease research areas for porcine models include the fields of transgenics and regenerative medicine. For the near term, pigs are expected to remain an important translational model to bridge the divide between traditional rodent models and humans in advancing biological experimentation and improving human health.
 
Which Luminex Assay is right for me?

Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.

Scientific Data

Porcine Luminex Discovery Panel Standard Curves

Porcine Luminex Discovery Assay Serum Sample Values Serum samples from 4 porcine were run on the Porcine Luminex Discovery Assay and sample values plotted. Minimum and maximum values shown by exterior lines, the box represents the 1st and 3rd quartiles with the center line representing the median.

Product Datasheets

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Product Specific Notices
Luminex® is a registered trademark of Luminex Corporation.

Citations for Porcine Luminex® Discovery Assay

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Gut microbiome perturbation and its correlation with tylosin pharmacokinetics in healthy and infected pigs
    Authors: Lee, EB;Lee, GY;Hossain, MA;Awji, EG;Park, SC;
    Scientific reports
    Species: Porcine
    Sample Types: Plasma
  2. First Application of a Mixed Porcine-Human Repopulated Bioengineered Liver in a Preclinical Model of Post-Resection Liver Failure
    Authors: Felgendreff, P;Hosseiniasl, SM;Minshew, A;Amiot, BP;Wilken, S;Ahmadzada, B;Huebert, RC;Sakrikar, NJ;Engles, NG;Halsten, P;Mariakis, K;Barry, J;Riesgraf, S;Fecteau, C;Ross, JJ;Nyberg, SL;
    Biomedicines
    Species: Porcine
    Sample Types: Serum
  3. Elamipretide mitigates ischemia-reperfusion injury in a swine model of hemorrhagic shock
    Authors: N Patel, MA Johnson, N Vapniarsky, MW Van Brockl, TK Williams, ST Youngquist, R Ford, N Ewer, LP Neff, GL Hoareau
    Scientific Reports, 2023-03-18;13(1):4496.
    Species: Porcine
    Sample Types: Serum

FAQs

  1. Are QC controls available for both the Luminex Discovery and High Performance Assays?

    • Controls are not offered for Luminex Discovery Assays, but may be formulated by the end user. A general guide may be available upon request. Controls are available for select Luminex High Performance Assays and can be found under the supplemental products tab on the product webpage. Some High Performance Luminex kits include controls and the controls will be listed in the materials provided section on the product datasheet. When included in the kit, controls are not sold separately.

  2. Why does the Luminex Assay Customization Tool provide the option to split based on dilution?

    • The feature to split based on dilution enables investigators to configure premix Luminex panels based on sample type (cell culture supernates, serum, and plasma). This keeps analytes requiring the same dilution factor within a single panel, while separating analytes requiring different dilutions. The recommended dilution factor for each analyte may be different for each sample type, and the suggested sample dilutions are built into the Luminex Customization Tool. Note that the suggested dilution factors are based on samples from healthy volunteers.

  3. Is the reconstituted standard a concentrate?

    • For Discovery Assays, each standard cocktail is a concentrate upon reconstitution with the volume specified on the Certificate of Analysis (CoA). After creating Standard 1 following the table in the product insert, the Standard 1 value is the concentration listed on the CoA.

  4. At what frequency should Calibration and Verification tests be performed?

    • It is recommended that a Calibration test be performed within one week prior to running an assay. A Verification test should be performed prior to data acquisition on the day of running an assay.

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