Home / IL-4 / Porcine IL-4 Antibody

Porcine IL-4 Antibody

Catalog #: MAB6543
1 Citation Datasheet

Discontinued Product

MAB6543 has been discontinued.
View all IL-4 products.
Detection of IL‑4 in PBMC lymphocytes by Flow Cytometry.
1 Image
Product Details
Citations (1)
FAQs
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Porcine IL-4 Antibody Summary

Species Reactivity
Porcine
Specificity
Detects porcine IL-4 in direct ELISAs and Western blots. Does not cross-react with recombinant human IL-13 or with recombinant IL-4 from human, canine, bovine, mouse, rat, cotton rat, or feline systems.
Source
Monoclonal Mouse IgG1 Clone # 99605
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant porcine IL-4
His25-Cys133
Accession # Q04745
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Porcine IL‑4 (Catalog # 654-P4)
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
PBMC lymphocytes fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry View Larger

Detection of IL‑4 in PBMC lymphocytes by Flow Cytometry. PBMC lymphocytes were stained with Mouse Anti-Porcine IL‑4 Monoclonal Antibody (Catalog # MAB6543, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-4

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 - 18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Porcine IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature porcine IL-4 shares 78%, 59%, 41%, and 41% aa sequence identity with bovine, human, mouse, and rat IL-4, respectively. Human IL-4 is active on porcine vascular endothelial cells (5). IL-4 exerts its effects through two receptor complexes (6, 7). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL‑2, ‑7, ‑9, ‑15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL‑4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgE in B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (8, 11). IL-4 plays a dominant role in the development of allergic inflammation and asthma (10, 12).

References
  1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
  2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
  3. Bailey, M. et al. (1993) Biochim. Biophys. Acta 1171:328.
  4. Redfield, C. et al. (1991) Biochemistry 30:11029.
  5. Stocker, C.J. et al. (2000) J. Immunol. 164:3309.
  6. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
  7. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
  8. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
  9. Corthay, A. (2006) Scand. J. Immunol. 64:93.
  10. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
  11. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
  12. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.
Long Name
Interleukin 4
Entrez Gene IDs
3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)
Alternate Names
B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; IL4E12; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra

Product Datasheets

You must select a language.

x
Product Datasheet

Citation for Porcine IL-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. TREM2 suppresses the proinflammatory response to facilitate PRRSV infection via PI3K/NF-kappaB signaling
    Authors: Z Zhu, X Zhang, W Dong, X Wang, S He, H Zhang, X Wang, R Wei, Y Chen, X Liu, C Guo
    PLoS Pathog., 2020-05-13;16(5):e1008543.
    Species: Porcine
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Immunoprecipitation, Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Porcine IL-4 Antibody

There are currently no reviews for this product. Be the first to review Porcine IL-4 Antibody and earn rewards!

Have you used Porcine IL-4 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review