Porcine CD34 Alexa Fluor® 594-conjugated Antibody

Catalog #: AF3890T Datasheet

Catalog #	Availability	Size / Price	Qty
AF3890T-100UG

All CD34 Antibodies

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Porcine CD34 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Porcine

Specificity

Detects porcine CD34 in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross‑reactivity with recombinant rat CD34 is observed, and less than 5% cross-reactivity with recombinant mouse CD34 and recombinant human CD34 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant porcine CD34
Ala20-Thr294 (Thr32Ala, Ala52Thr, Pro71Ser, Val126Ala, His278Asn)
Accession # NP_999251

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: CD34

CD34 is a 110 kDa type I transmembrane glycoprotein that belongs to the CD34/Podocalyxin family of sialomucin. It is a widely used marker of activated hematopoietic stem/progenitor cells and is also expressed on adult vascular endothelial cells. Porcine CD34 is 389 amino acids (aa) in length and is predicted to have a 272 aa extracellular domain (ECD) and a 73 aa cytoplasmic tail. An alternate 307 aa splice form with a 4 aa substitution for the C-terminal 61 amino acids also exists. Over aa 20-294, porcine CD34 shares 51%, 57% and 62% aa sequence identity with mouse, human, and canine CD34, respectively.

Entrez Gene IDs

947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine)

Alternate Names

CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1