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Porcine CD31/PECAM-1 Antibody

Catalog #: MAB33871
6 Citations 1 Review Datasheet
Catalog # Availability Size / Price Qty
MAB33871
MAB33871-SP
Detection of CD31/PECAM‑1 in Porcine PBMCs by Flow Cytometry.
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Citations (6)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Porcine CD31/PECAM-1 Antibody Summary

Species Reactivity
Porcine
Specificity
Detects porcine CD31/PECAM‑1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CD31, recombinant mouse (rm) CD31, rhVCAM-1, rhICAM-1, rhICAM-2, rhICAM-3, rmICAM-5, or rmMADCAM-1 is observed.
Source
Monoclonal Rat IgG1 Clone # 377537
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant porcine CD31/PECAM‑1
Gln28-Lys602 (predicted)
Accession # Q95242
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Porcine CD31/PECAM‑1 (Catalog # 3387-PC)
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD31/PECAM-1 antibody in Porcine PBMCs antibody by Flow Cytometry. View Larger

Detection of CD31/PECAM‑1 in Porcine PBMCs by Flow Cytometry. Porcine peripheral blood mononuclear cells were stained with Rat Anti-Porcine CD31/PECAM-1 Monoclonal Antibody (Catalog # MAB33871, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0105B).

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD31/PECAM-1

CD31, also known as PECAM-1 (platelet-endothelial cell adhesion molecule-1), is a 130 kDa type I transmembrane glycoprotein adhesion molecule in the immunoglobulin superfamily (1, 2). Expression is restricted to the vascular system, especially endothelial cells, platelets, monocytes, neutrophils and lymphocyte subsets. CD31 is concentrated at cell-cell junctions and is required for transendothelial migration (TEM) (1‑3). The extracellular domain (ECD) of CD31 has ten potential N-glycosylation sites and six C2-type Ig-like domains, the first of which is critical for adhesion and extravasation (3, 4). The cytoplasmic domain contains immunoregulatory tyrosine-based inhibitory and switch motifs (ITIM, ITSM) that mediate both inhibition and activation via phosphotyrosine-mediated engagement of SH2-containing signaling molecules (1, 5). Metalloproteinase-mediated ectodomain shedding occurs during apoptosis (6) but increased serum CD31 ectodomain in HIV and active multiple sclerosis occurs independent of apoptosis (7, 8). In humans, expression of six isoforms with exon deletions in the cytoplasmic domain is tissue‑ and stage-specific, but full-length CD31 is predominant. A form lacking the ITSM predominates in mouse (9). Porcine CD31 ECD shows 74%, 73%, 70%, 63% and 62% amino acid (aa) identity with bovine, canine, human, mouse and rat CD31, respectively. CD31 participates with other adhesion molecules for most functions but is the critical molecule for TEM. Homotypic CD31 adhesion in trans combined with cycling of CD31 to and from surface-connected endothelial cell vesicles leads leukocytes across endothelial tight junctions (3, 10). Homotypic adhesion and signaling functions also strongly suppress mitochondria-dependent apoptosis (11). In platelets, PECAM-1 is necessary for limiting thrombus formation (12) and promoting integrin-mediated clot retraction and platelet spreading (13), but mechanisms for these phenomena are unclear. CD31-/- mice are deficient in chemokine-mediated chemotaxis (14).

References
  1. Ilan, N. and J.A. Madri (2003) Curr Opin. Cell Biol. 15:515.
  2. Nasu, K. et al. (1999) Transplantation 68:861.
  3. Liao, F. et al. (1997) J. Exp. Med. 185:1349.
  4. Nakada, M.T. et al. (2000) J. Immunol. 164:452.
  5. Chemnitz, J.M. et al. (2004) J. Immunol. 173:945.
  6. Ilan, N. et al. (2001) FASEB J. 15:362.
  7. Eugenin, E.A. et al. (2006)J. Leukoc. Biol. 79:444.
  8. Losy, J. et al. (1999) 99:169.
  9. Wang, Y. et al. (2003) Am. J. Physiol. Heart Circ. Physiol. 284:H1008.
  10. Mamdouh, Z. et al. (2003) Nature 421:748.
  11. Gao, C. et al. (2003) Blood 102:169.
  12. Falati, S. et al. (2006) Blood 107:535.
  13. Wee, J. L. and D.E. Jackson (2005) Blood 106:3816.
  14. Wu, Y. et al. (2005) J. Immunol. 175:3484.
Long Name
Platelet Endothelial Cell Adhesion Molecule 1
Entrez Gene IDs
5175 (Human); 18613 (Mouse); 29583 (Rat)
Alternate Names
adhesion molecule; CD31 antigen; CD31; CD31/EndoCAM; EndoCAM; FLJ34100; FLJ58394; GPIIA'; PECA1; PECAM1; PECAM-1; PECAM-1, CD31/EndoCAM; platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule-1; platelet/endothelial cell adhesion molecule

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Citations for Porcine CD31/PECAM-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Combination of Anti-CD40 and Anti-CD40L Antibodies as Co-Stimulation Blockade in Preclinical Cardiac Xenotransplantation
    Authors: Bender, M;Abicht, JM;Reichart, B;Neumann, E;Radan, J;Mokelke, M;Buttgereit, I;Leuschen, M;Wall, F;Michel, S;Ellgass, R;Steen, S;Paskevicius, A;Lange, A;Kessler, B;Kemter, E;Klymiuk, N;Denner, J;Godehardt, AW;Tönjes, RR;Burgmann, JM;Figueiredo, C;Milusev, A;Zollet, V;Salimi-Afjani, N;Despont, A;Rieben, R;Ledderose, S;Walz, C;Hagl, C;Ayares, D;Wolf, E;Schmoeckel, M;Brenner, P;Binder, U;Gebauer, M;Skerra, A;Längin, M;
    Biomedicines
    Species: Transgenic Porcine, Xenograft
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. An antibody against Siglec-15 promotes bone formation and fracture healing by increasing TRAP+ mononuclear cells and PDGF-BB secretion
    Authors: Gehua Zhen, Yang Dan, Ruomei Wang, Ce Dou, Qiaoyue Guo, Melissa Zarr et al.
    Bone Research
  3. In Situ Bioprinting of Autologous Skin Cells Accelerates Wound Healing of Extensive Excisional Full-Thickness Wounds
    Authors: M Albanna, KW Binder, SV Murphy, J Kim, SA Qasem, W Zhao, J Tan, IB El-Amin, DD Dice, J Marco, J Green, T Xu, A Skardal, JH Holmes, JD Jackson, A Atala, JJ Yoo
    Sci Rep, 2019-02-12;9(1):1856.
    Species: Porcine
    Sample Types: Whole Tissue
    Applications: IHC-Fr, IHC-P
  4. 3D artificial round section micro-vessels to investigate endothelial cells under physiological flow conditions
    Authors: R Sfriso, S Zhang, CA Bichsel, O Steck, A Despont, OT Guenat, R Rieben
    Sci Rep, 2018-04-12;8(1):5898.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: ICC
  5. Surface modification of pig endothelial cells with a branched heparin conjugate improves their compatibility with human blood
    Authors: AK Bongoni, E Salvaris, S Nordling, N Klymiuk, E Wolf, DL Ayares, R Rieben, PU Magnusson, PJ Cowan
    Sci Rep, 2017-06-30;7(1):4450.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: ICC
  6. Effect of C1-INH on ischemia/reperfusion injury in a porcine limb ex vivo perfusion model
    Authors: MM Abdelhafez, J Shaw, D Sutter, J Schnider, Y Banz, H Jenni, E Voegelin, MA Constantin, R Rieben
    Mol. Immunol., 2017-06-20;88(0):116-124.
    Species: Porcine
    Sample Types: Whole Tissue
    Applications: IHC

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Porcine CD31/PECAM-1 Antibody
By Anonymous on 11/24/2021
Application: WB Sample Tested: Endothelial cells Species: Porcine
Western Blot Porcine CD31/PECAM-1 Antibody MAB33871