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Poly(I:C)

Catalog #: 4287
20 Citations 1 Review Datasheet
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Poly(I:C) | CAS No. 42424-50-0 | Toll-like Receptor Agonists
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Description: TLR3 agonist

Chemical Name: Polyinosinic-polycytidylic acid sodium salt

Product Details
Citations (20)
Supplemental Products
Reviews (1)
Biological Activity Technical Data Additional Information Background Product Datasheets Calculators

Biological Activity

Poly(I:C) is a synthetic double-stranded RNA (dsRNA); endosomal Toll-like receptor 3 (TLR3) agonist. It also binds to the cytoplasmic receptors, retinoic acid-inducible gene I (RIG-I) and melanoma differentiation-associated gene 5 (MDA-5), elicits the secretion of type I interferon (IFN) and pro-inflammatory cytokines. Poly(I:C) is commonly used for immune system activation in vivo. Poly(I:C) also promotes maturation of hPSC-derived cardiomyocytes by modulating early Notch signaling and cardiomyogenic transcriptional programs. Poly(I:C) promotes generation of stable, mature dendritic cells in combination with TLR8 agonist CL 075 (Cat. No. 6142). Transfection into NIT-1 β cells induces β-cell apoptosis.

To prevent denaturation, this product should be reconstituted in solutions with physiological salt concentrations.

Technical Data

Formula:
(C10H13N4O8P)x.(C9H14N3O8P)x
Solubility:
Soluble to 3 mg/ml in water
Storage:
Store at -20°C
CAS No:
42424-50-0

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Other Product-Specific Information:
  • To prevent denaturation this product should be reconstituted in solutions with physiological salt concentrations.

Background References

  1. Cetuximab-activated natural killer and dendritic cells collaborate to trigger tumor antigen-specific T-cell immunity in head and neck cancer patients.
    Srivastava R, Lee S, Andrade Filho P, Lord C, Jie H, Davidson H, Lopez-Albaitero A, Gibson S, Gooding W, Ferrone S, Ferris R
    Clin Cancer Res, 2013;19(7):1858-72.
  2. Epidermal Expression and Regulation of Interleukin-33 during Homeostasis and Inflammation: Strong Species Differences.
    Sundnes O, Pietka W, Loos T, Sponheim J, Rankin A, Pflanz S, Bertelsen V, Sitek J, Hol J, Haraldsen G, Khnykin D
    J Invest Dermatol, 2015;135(7):1771-80.
  3. Decreased TLR3 in Hyperplastic Adipose Tissue, Blood and Inflamed Adipocytes is Related to Metabolic Inflammation
    J Latorre, JM Moreno-Nav, M Sabater, M Buxo, JI Rodriguez-, J Girones, JM Fort, R Vilallonga, W Ricart, R Simo, JM Fernandez-, FJ Ortega
    Cell. Physiol. Biochem., 2018;51(3):1051-1068.
  4. The Efficacy of the Interferon Alpha/Beta Response versus Arboviruses Is Temperature Dependent
    WC Lane, MD Dunn, CL Gardner, LKM Lam, AM Watson, AL Hartman, KD Ryman, WB Klimstra
    MBio, 2018;9(2):.
  5. Duck Tembusu Virus Nonstructural Protein 1 Antagonizes IFN-? Signaling Pathways by Targeting VISA
    Qiyun Zhu
    J. Immunol., 2016;0(0):.
  6. RNA-Eluting Surfaces for the Modulation of Gene Expression as A Novel Stent Concept
    O Koenig, D Zengerle, N Perle, S Hossfeld, B Neumann, A Behring, M Avci-Adali, T Walker, C Schlensak, HP Wendel, A Nolte
    Pharmaceuticals (Basel), 2017;10(1):.
  7. Interleukin-20 is triggered by TLR ligands and associates with disease activity in patients with rheumatoid arthritis
    L Šenolt, K Prajzlerov, H Hulejová, B Šumová, M Filková, D Veigl, K Pavelka, J Vencovský
    Cytokine, 2017;97(0):187-192.
  8. Generation of Th1-polarizing dendritic cells using the TLR7/8 agonist CL075.
    Spranger et al.
    J.Immunol., 2010;185:738
  9. Nuclear factor-κB translocation mediates double-stranded ribonucleic acid-induced NIT-1 beta-cell apoptosis and up-regulates caspase-12 and tumor necrosis factor-associated ligand (TRAIL).
    Robbins et al.
    Endocrinology, 2003;144:4616
  10. Poly(I:C) used for human dendritic cell maturation preserves their ability to secondarily secrete bioactive IL-12.
    Rouas et al.
    Int.Immunol., 2004;16:767
  11. TLR signalling augments macrophage bactericidal activity through mitochondrial ROS.
    West et al.
    Nature, 2011;472:476
  12. Epigenetic priming of human pluripotent stem cell-derived cardiac progenitor cells accelerates cardiomyocyte maturation.
    Biermann et al.
    Stem Cells, 2019;37:910
  13. Ectonucleotidase CD39 is highly expressed on ATLL cells and is responsible for their immunosuppressive function
    Y Nagate, S Ezoe, J Fujita, D Okuzakis, D Motooka, T Ishibashi, M Ichii, A Tanimura, M Kurashige, E Morii, T Fukushima, Y Suehiro, T Yokota, H Shibayama, K Oritani, Y Kanakura
    Leukemia, 2020;0(0):.
  14. Enhanced 15-Lipoxygenase 1 Production is Related to Periostin Expression and Eosinophil Recruitment in Eosinophilic Chronic Rhinosinusitis
    Y Imoto, T Takabayash, M Sakashita, Y Kato, K Yoshida, M Kidoguchi, K Koyama, N Adachi, Y Kimura, K Ogi, Y Ito, M Kanno, M Okamoto, N Narita, S Fujieda
    Biomolecules, 2020;10(11):.

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Citations for Poly(I:C)

The citations listed below are publications that use Tocris products. Selected citations for Poly(I:C) include:

20 Citations: Showing 1 - 10

  1. Anti-inflammatory properties of antiangiogenic fucoidan in retinal pigment epithelium cells.
    Authors: Alexa Et al.
    Heliyon  2023;9:e15202
  2. Genetic adaptation to pathogens and increased risk of inflammatory disorders in post-Neolithic Europe.
    Authors: Anne Et al.
    Cell Genom  2023;3:100248
  3. A p53-TLR3 axis ameliorates pulmonary hypertension by inducing BMPR2 via IRF3.
    Authors: Mingjun Et al.
    iScience  2023;26:105935
  4. Weight cycling induces innate immune memory in adipose tissue macrophages.
    Authors: Alyssa H Et al.
    Front Immunol  2023;13:984859
  5. Assessing human iPSC-derived microglia identity and function by immunostaining, phagocytosis, calcium activity, and inflammation assay.
    Authors: Sandra Et al.
    STAR Protoc  2022;3:101866
  6. TRAF6 functions as a tumor suppressor in myeloid malignancies by directly targeting MYC oncogenic activity.
    Authors: Scott W Et al.
    Cell Stem Cell  2022;29:298-314.e9
  7. Poly I:C and R848 facilitate nitric oxide production via inducible nitric oxide synthase in chicks.
    Authors: Tachibana Et al.
    Comp.Biochem.Physiol.A Mol.Integr.Physiol.  2022;269:111211
  8. Pro-inflammatory activation changes intracellular transport of bevacizumab in the retinal pigment epithelium in vitro.
    Authors: Johann Et al.
    Graefes Arch Clin Exp Ophthalmol  2022;260:857-872
  9. Stem cell-derived porcine macrophages as a new platform for studying host-pathogen interactions.
    Authors: Xuefei Et al.
    BMC Biol  2022;20:14
  10. Human Lung Fibroblasts Exhibit Induced Inflammation Memory via Increased IL6 Gene Expression and Release.
    Authors: Takashi Et al.
    Front Immunol  2022;13:921728
  11. ADAM17 Boosts Cholesterol Efflux and Downstream Effects of High-Density Lipoprotein on Inflammatory Pathways in Macrophages.
    Authors: Yi Et al.
    Arterioscler Thromb Vasc Biol  2021;41:1854-1873
  12. Virus Infection Induces Keap1 Binding to Cytokine Genes, Which Recruits NF-κB p50 and G9a-GLP and Represses Cytokine Transcription.
    Authors: Veronica Elizabeth Et al.
    J Immunol  2021;207:1437-1447
  13. PERK Is Critical for Alphavirus Nonstructural Protein Translation.
    Authors: Ivan Et al.
    Viruses  2021;13
  14. EZH2 Inhibition Compromises α4-1BB-Mediated Antitumor Efficacy by Reducing the Survival and Effector Programming of CD8+ T Cells.
    Authors: Shahram Et al.
    Front Immunol  2021;12:770080
  15. TRIM21 Dysfunction Enhances Aberrant B-Cell Differentiation in Autoimmune Pathogenesis.
    Authors: Hideaki Et al.
    Front Immunol  2020;11:98
  16. IL-17c is involved in olfactory mucosa responses to Poly(I:C) mimicking virus presence.
    Authors: Nicolas Et al.
    Brain Behav Immun  2019;79:274-283
  17. Microglial nodules provide the environment for pathogenic T cells in human encephalitis.
    Authors: Tröscher Et al.
    Acta Neuropathol  2019;137:619
  18. Cryo-EM Structures of MDA5-dsRNA Filaments at Different Stages of ATP Hydrolysis.
    Authors: Yu Et al.
    Mol Cell  2018;72:999
  19. PDGFRβ Cells Rapidly Relay Inflammatory Signal from the Circulatory System to Neurons via Chemokine CCL2.
    Authors: Yue Et al.
    Neuron  2018;100:183-200.e8
  20. Systemic Activation of TLR3-Dependent TRIF Signaling Confers Host Defense against Gram-Negative Bacteria in the Intestine.
    Authors: Masayuki Et al.
    Front Cell Infect Microbiol  2016;5:105

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Good product
By KANG LE on 04/24/2018

I used this product to activate TLR3.

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