Phospho-GSK-3 alpha/beta (S21/S9) DuoSet IC ELISA

Catalog #: DYC2630-2 Datasheet

Catalog #	Availability	Size / Price	Qty
DYC2630-5
DYC2630-2

Ancillary Products Available

DY999B: Substrate Reagent Pack

DY999: Substrate Reagent Pack

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All GSK-3 alpha/beta ELISAs

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Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Phospho-GSK-3 alpha/beta (S21/S9) DuoSet IC ELISA Summary

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4 hours 40 minutes (after plate preparation)

Sample Volume Required

Cell lysates (100 µL)

Assay Range

156.0 - 10,000 pg/mL

Sufficient Materials

Kits available for two, five, or fifteen 96-well plates*

Specificity

Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure phosphorylated human, mouse, and rat GSK-3 alpha / beta (S21 / S9) in cell lysates. An immobilized capture antibody specific for GSK-3 alpha / beta binds both phosphorylated and unphosphorylated GSK-3 alpha / beta. After washing away unbound material, a biotinylated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format.

Product Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Available in 2, 5, and 15- (96-well) plate pack sizes
Economical alternative to Western blot

Kit Content

Capture Antibody
Conjugated Detection Antibody
Calibrated Immunoassay Standard or Control
Streptavidin-HRP

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂O₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Scientific Data

ELISA

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Standard Curve

Product Datasheets

Product Datasheet

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: GSK-3 alpha/beta

GSK-3 is a Ser/Thr kinase first identified as an inactivator of Glycogen Synthase. GSK-3 acts as a multifunctional downstream switch that determines the output of numerous signaling pathways. There are two mammalian GSK-3 isoforms encoded by distinct genes, GSK-3 alpha and GSK-3 beta, which are structurally similar, but functionally non-identical. GSK-3a is inhibited by phosphorylation at S21 by Akt and other kinases. GSK-3 alpha and GSK-3 beta share 85% amino acid identity. Dysregulated GSK-3 has been implicated in several diseases including type II diabetes, Alzheimer's disease, bipolar disorder, and cancer.

Long Name:

Glycogen Synthase Kinase 3

Alternate Names:

DKFZp686D0638; EC 2.7.11; EC 2.7.11.26; glycogen synthase kinase 3 alpha; glycogen synthase kinase-3 alpha; GSK-3 alpha; GSK-3 alpha/beta