Phospho-Akt (S473) PE-conjugated Antibody

Catalog #: IC7794P 1 Review Datasheet

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IC7794P

Detection of Phospho-Akt (S473) in Jurkat Human Cell Line by Flow Cytometry.

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Phospho-Akt (S473) PE-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse Akt1, Akt2 and Akt3, when phosphorylated at S473, S474 and S472, respectively.

Source

Monoclonal Mouse IgG₁ Clone # 545007

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Phosphopeptide containing the human Akt (S473) site

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of Phospho-Akt (S473) antibody in Jurkat Human Cell Line antibody by Flow Cytometry.

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Detection of Phospho-Akt (S473) in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line either untreated (open histogram) or treated with 100 nM Calyculin A (Catalog # 1336) for 30 minutes (filled histogram) was stained with Mouse Anti-Human/Mouse Phospho-Akt (S473) PE-conjugated Monoclonal Antibody (Catalog # IC7794P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Akt

Akt, also known as Protein Kinase B (PKB), is a central kinase in such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. Three highly homologous members define the Akt family: Akt1 (PKB alpha ), Akt2 (PKB beta ), and Akt3 (PKB gamma ). All three Akts contain an amino-terminal pleckstrin homology domain, a central kinase domain, and a carboxyl-terminal regulatory domain. Akt1 is the most widely expressed family member and is frequently activated in a number of carcinomas, including breast, prostate, lung, pancreatic, liver, ovarian, and colorectal cancer. Akt1 is activated in a multistep process that involves the sequential phosphorylation of Thr450 by JNK kinases, Thr308 by PDK1, and Ser473 by PDK2 or mTORC2. Activated Akt1 phosphorylates a wide variety of cytosolic, nuclear, and mitochondrial substrates. Human Akt1 shares 98% aa sequence identity with mouse and rat Akt1.

Long Name

v-Akt Murine Thymoma Viral Oncogene Homolog

Alternate Names

RAC-ALPHA; AKT serine/threonine kinase 1; Akt; PKB; PKB-ALPHA; PRKBA; RAC