PEI STAR™ transfection reagent
Chemical Name: Poly[imino(1,2-ethanediyl)] hydrochloride
Biological Activity
PEI STAR™ is a chemically-defined, high-performance polyethylenimine (PEI) transfection reagent for cost-effective, affordable and scalable transient gene expression. PEI is a synthetic polymer with an exceptionally high positive charge density in pH-neutral solutions. Positively charged PEI binds strongly to negatively charged DNA and imparts a net cationic charge, allowing the DNA to enter cells. PEI is a non-viral vector commonly used to transfect HEK293 and CHO cells. Applications include production of recombinant proteins, antibodies and viruses.Pre-dissolved, ready-to-use formulation of PEI STAR™ also available: PEI STAR-Go™ transfection reagent (Cat. No. 8174).
PEI STAR is a trademark of Bio-Techne Corp.
Scientific Data
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Application of PEI STAR™ transfection reagent. PEI STAR™ performance data comparison (HEK293): HEK 293 - 20 mL cultures containing HEK293 suspensions were transfected with a CMV-SEAP plasmid at optimized PEI/DNA ratios using either PEI STAR™ (3:1) or leading competitor PEI. SEAP expression levels were quantified 5 days post-transfection using phosphatase reporter dye and UV/Vis absorbance.
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Application of PEI STAR™ transfection reagent. PEI STAR™ performance data comparison (CHO): CHO - 20 mL cultures containing CHO suspensions were transfected with a CMV-SEAP plasmid at optimized PEI/DNA ratios using either PEI STAR™ (5:1) or leading competitor PEI. SEAP expression levels were quantified 5 days post-transfection using phosphatase reporter dye and UV/Vis absorbance.
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Transfection of a reporter GFP construct using a leading competitor PEI and PEI STAR™. HEK293 cells grown in DMEM/10% FBS to 75% confluency in a 24 well plate. 1 μg of DNA + 25 μL optimem and 2 μg of PEI (1 mg/mL) + 25 μL optimem were incubated for 8 minutes before addition to cells. PEI STAR™ showed significantly higher expression of GFP in imaged cells.
Protocols
Please see the links below for protocols relating to the use of PEI STAR™
| Protocol Name | Cell Type |
|---|---|
| PEI STAR™ Transfection Reagent preparation | |
| Gene Expression in Adherent HEK293 cells | Adherent |
| rAAV Production in Adherent HEK293 Cells | Adherent |
| Lentivirus Production in Adherent HEK293T Cells | Adherent |
| Gene Expression in HEK293 Cell Suspensions | Suspension |
| rAAV Production in Suspension HEK293 Cells | Suspension |
| Gene Expression in CHO Suspensions | Suspension |
Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets
Citation for PEI STAR™ transfection reagent
The citations listed below are publications that use Tocris products. Selected citations for PEI STAR™ transfection reagent include:
1 Citation: Showing 1 - 1
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Surface functionalization of virus-like particles via bioorthogonal click reactions for enhanced cell-specific targeting.
Authors: Laomeephol Et al.
Int.J.Pharm. 2024;660:124332
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