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PARP Universal Colorimetric Assay Kit

Catalog #: 4677-096-K
33 Citations Datasheet
For the in vitro screening of candidate PARP-1 inhibitors
Catalog # Availability Size / Price Qty
4677-096-K
Inhibition of PARP Activity by 3-aminobenzamide_4677-096-K
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Citations (33)
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PARP Universal Colorimetric Assay Kit Summary

Ideal for the in vitro screening of candidate PARP-1 inhibitors and determination of IC50 values.
 

Key Benefits

• Colorimetric readout
• 96 strip-well format
• Sensitive – detects 10 mU PARP /well
• Assay Time ~3 hrs

Why Use the PARP/Apoptosis Colorimetric Assay Kit?

This ELISA based assay detects biotinylated poly (ADP-ribose) deposited by PARP-1 onto immobilized histones in a 96-well format. The addition of Strep-HRP (biotin-binding protein) and a colorimetric HRP substrate yields relative absorbance that correlates with PARP-1 activity.

Product Specifications

For the in vitro screening of candidate PARP-1 inhibitors and determination of IC50 values.

Kit Contents

• 3-Aminobenzamide
• PARP-HSA, 10 Units/µl
• 20X PARP Buffer
• 10X PARP Cocktail
• 10X Activated DNA
• 10X Strep-Diluent
• Histone-Coated Plate Natural Strip Well Plate
• Strep-HRP
• TACS-Sapphire

Specifications

Shipping Conditions
The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Multi-species

Limitations

For research use only. Not for diagnostic use.

Product Datasheets

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Scientific Data

N/A Inhibition of PARP Activity by 3-aminobenzamide_4677-096-K View Larger

Inhibition of PARP Activity by 3-aminobenzamide. Graphical representation of the colorimetric readout of a PARP standard curve (A) and an inhibition curve for the PARP inhibitor 3-aminobenzamide (provided in the kit). (B) The standard curve is used to translate absorbance values to activity units of PARP.

Citations for PARP Universal Colorimetric Assay Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

33 Citations: Showing 1 - 10
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  1. Design, synthesis and development of a dual inhibitor of Topoisomerase 1 and poly (ADP-ribose) polymerase 1 for efficient killing of cancer cells
    Authors: Guha Majumdar, A;Shree, S;Das, A;Kumar, BK;Dey, P;Subramanian, M;Patro, BS;
    European journal of medicinal chemistry  2023-10-05
  2. Role of PARP and TRPM2 in VEGF Inhibitor-Induced Vascular Dysfunction
    Authors: KB Neves, R Alves-Lope, AC Montezano, RM Touyz
    Journal of the American Heart Association, 2023-02-20;0(0):e027769.  2023-02-20
  3. Natural variations of ZmSRO1d modulate the trade-off between drought resistance and yield by affecting ZmRBOHC-mediated stomatal ROS production in maize
    Authors: H Gao, J Cui, S Liu, S Wang, Y Lian, Y Bai, T Zhu, H Wu, Y Wang, S Yang, X Li, J Zhuang, L Chen, Z Gong, F Qin
    Molecular plant, 2022-08-31;0(0):.  2022-08-31
  4. BRCA mutations lead to XIAP overexpression and sensitise ovarian cancer to inhibitor of apoptosis (IAP) family inhibitors
    Authors: M Cremona, CJ Vandenberg, AM Farrelly, SF Madden, C Morgan, R Kalachand, JN McAlpine, S Toomey, DG Huntsman, L Grogan, O Breathnach, P Morris, MS Carey, CL Scott, BT Hennessy
    British Journal of Cancer, 2022-04-30;0(0):.  2022-04-30
  5. TET-mediated DNA hydroxymethylation is negatively influenced by the PARP-dependent PARylation
    Authors: A Toli?, M Ravichandr, J Raji?, M ?or?evi?, M ?or?evi?, S Dini?, N Grdovi?, JA Jovanovi?, M Mihailovi?, N Nestorovi?, TP Jurkowski, AS Uskokovi?, MS Vidakovi?
    Epigenetics & Chromatin, 2022-04-05;15(1):11.  2022-04-05
  6. JAK Inhibition Prevents DNA Damage and Apoptosis in Testicular Ischemia-Reperfusion Injury via Modulation of the ATM/ATR/Chk Pathway
    Authors: F Khashab, F Al-Saleh, N Al-Kandari, F Fadel, M Al-Maghreb
    International Journal of Molecular Sciences, 2021-12-13;22(24):.  2021-12-13
  7. Cytocidal Antitumor Effects against Human Ovarian Cancer Cells Induced by B-Lactam Steroid Alkylators with Targeted Activity against Poly (ADP-Ribose) Polymerase (PARP) Enzymes in a Cell-Free Assay
    Authors: N Nikoleousa, P Dalezis, A Polonifi, EG Geromichal, S Sagredou, CE Alifieris, MV Deligiorgi, V Sarli, DT Trafalis
    Biomedicines, 2021-08-17;9(8):.  2021-08-17
  8. TSL-1502, a glucuronide prodrug of a poly (ADP-ribose) polymerase (PARP) inhibitor, exhibits potent anti-tumor activity in preclinical models
    Authors: L Wang, X Zhu, L Li, L Li, L Fu, Y Li, H Fu, X Chen, L Lou
    American journal of cancer research, 2021-04-15;11(4):1632-1645.  2021-04-15
  9. Yin and Yang Regulation of Liver X Receptor &alpha Signaling Control of Cholesterol Metabolism by Poly(ADP-ribose) polymerase 1
    Authors: F Zhang, C Wang, Y Jiang, K Huang, F Liu, M Du, X Luo, D Huang, K Huang
    Int J Biol Sci, 2020-09-01;16(15):2868-2882.  2020-09-01
  10. Molecular signatures of BRCAness analysis identifies PARP inhibitor Niraparib as a novel targeted therapeutic strategy for soft tissue Sarcomas
    Authors: H Li, J Tu, Z Zhao, L Chen, Y Qu, H Li, H Yao, X Wang, DF Lee, J Shen, L Wen, G Huang, X Xie
    Theranostics, 2020-07-25;10(21):9477-9494.  2020-07-25
  11. Inhibition of NADPH oxidase alleviates germ cell apoptosis and ER stress during testicular ischemia reperfusion injury
    Authors: F Al-Saleh, F Khashab, F Fadel, N Al-Kandari, M Al-Maghreb
    Saudi J Biol Sci, 2020-04-21;27(8):2174-2184.  2020-04-21
  12. Riluzole protects against skeletal muscle ischaemia-reperfusion injury in a porcine model
    Authors: RW Li, Y Deng, HN Pham, S Weiss, M Chen, PN Smith
    Injury, 2019-12-17;0(0):.  2019-12-17
  13. Increased error-free DNA repair gene expression through reprogramming in human iPS cells
    Authors: Y Yoshimura
    Regen Ther, 2019-06-28;11(0):101-105.  2019-06-28
  14. The Effect of Green and Black Tea Polyphenols on BRCA2 Deficient Chinese Hamster Cells by Synthetic Lethality through PARP Inhibition
    Authors: S Alqahtani, K Welton, JP Gius, S Elmegerhi, TA Kato
    Int J Mol Sci, 2019-03-14;20(6):.  2019-03-14
  15. Poly(ADP-ribose) polymerase 1 accelerates vascular calcification by upregulating Runx2
    Authors: C Wang, W Xu, J An, M Liang, Y Li, F Zhang, Q Tong, K Huang
    Nat Commun, 2019-03-13;10(1):1203.  2019-03-13
  16. Targeting on poly(ADP-ribose) polymerase activity with DNA-damaging hybrid lactam-steroid alkylators in wild-type and BRCA1-mutated ovarian cancer cells.
    Authors: Trafalis D, Polonifi A, Dalezis P, Nikoleousakos N, Katsamakas S, Sarli V
    Chem Biol Drug Des, 2017-05-26;90(5):854-866.  2017-05-26
  17. PARP inhibition ameliorates nephropathy in an animal model of type 2 diabetes: focus on oxidative stress, inflammation, and fibrosis.
    Authors: Zakaria E, El-Maraghy N, Ahmed A, Ali A, El-Bassossy H
    Naunyn Schmiedebergs Arch Pharmacol, 2017-02-21;390(6):621-631.  2017-02-21
  18. Acyl-CoA-binding domain containing 3 modulates NAD+ metabolism through activating poly(ADP-ribose) polymerase 1.
    Authors: Chen Y, Bang S, Park S, Shi H, Kim S
    Biochem J, 2015-05-05;469(2):189-98.  2015-05-05
  19. Disruption of Wnt/beta-Catenin Signaling and Telomeric Shortening Are Inextricable Consequences of Tankyrase Inhibition in Human Cells.
    Authors: Kulak O, Chen H, Holohan B, Wu X, He H, Borek D, Otwinowski Z, Yamaguchi K, Garofalo L, Ma Z, Wright W, Chen C, Shay J, Zhang X, Lum L
    Mol Cell Biol, 2015-05-04;35(14):2425-35.  2015-05-04
  20. Silencing of poly(ADP-ribose) polymerase-1 suppresses hyperstretch-induced expression of inflammatory cytokines in vitro.
    Authors: Wang J, Liu L, Xia Y, Wu D
    Acta Biochim Biophys Sin (Shanghai), 2014-05-14;46(7):556-64.  2014-05-14
  21. The PARP inhibitors, veliparib and olaparib, are effective chemopreventive agents for delaying mammary tumor development in BRCA1-deficient mice.
    Authors: To C, Kim E, Royce D, Williams C, Collins R, Risingsong R, Sporn M, Liby K
    Cancer Prev Res (Phila), 2014-05-09;7(7):698-707.  2014-05-09
  22. New insights into PARP inhibitors' effect on cell cycle and homology-directed DNA damage repair.
    Authors: Jelinic P, Levine D
    Mol Cancer Ther, 2014-04-02;13(6):1645-54.  2014-04-02
  23. Combinatorial effects of PARP inhibitor PJ34 and histone deacetylase inhibitor vorinostat on leukemia cell lines.
    Authors: Jasek E, Gajda M, Lis G, Jasinska M, Litwin J
    Anticancer Res, 2014-04-01;34(4):1849-56.  2014-04-01
  24. Effect of small-molecule modification on single-cell pharmacokinetics of PARP inhibitors.
    Authors: Thurber G, Reiner T, Yang K, Kohler R, Weissleder R
    Mol Cancer Ther, 2014-02-19;13(4):986-95.  2014-02-19
  25. Poly(ADP-ribose) polymerase 1 promotes oxidative-stress-induced liver cell death via suppressing farnesoid X receptor alpha.
    Authors: Wang C, Zhang F, Wang L, Zhang Y, Li X, Huang K, Du M, Liu F, Huang S, Guan Y, Huang D, Huang K
    Mol Cell Biol, 2013-09-16;33(22):4492-503.  2013-09-16
  26. TRAIL induces necroptosis involving RIPK1/RIPK3-dependent PARP-1 activation.
    Authors: Jouan-Lanhouet S, Arshad M, Piquet-Pellorce C, Martin-Chouly C, Le Moigne-Muller G, Van Herreweghe F, Takahashi N, Sergent O, Lagadic-Gossmann D, Vandenabeele P, Samson M, Dimanche-Boitrel M
    Cell Death Differ, 2012-07-20;19(12):2003-14.  2012-07-20
  27. BCL2 suppresses PARP1 function and nonapoptotic cell death.
    Authors: Dutta C, Day T, Kopp N, van Bodegom D, Davids M, Ryan J, Bird L, Kommajosyula N, Weigert O, Yoda A, Fung H, Brown J, Shapiro G, Letai A, Weinstock D
    Cancer Res, 2012-06-11;72(16):4193-203.  2012-06-11
  28. Differential effects of poly(ADP-ribose) polymerase inhibition on DNA break repair in human cells are revealed with Epstein-Barr virus.
    Authors: Ma W, Halweg C, Menendez D, Resnick M
    Proc Natl Acad Sci U S A, 2012-04-09;109(17):6590-5.  2012-04-09
  29. Cannabinoid 1 receptor promotes cardiac dysfunction, oxidative stress, inflammation, and fibrosis in diabetic cardiomyopathy.
    Authors: Rajesh M, Batkai S, Kechrid M, Mukhopadhyay P, Lee W, Horvath B, Holovac E, Cinar R, Liaudet L, Mackie K, Hasko G, Pacher P
    Diabetes, 2012-02-07;61(3):716-27.  2012-02-07
  30. Nitric oxide modulates hypoxia-inducible factor-1 and poly(ADP-ribose) polymerase-1 cross talk in response to hypobaric hypoxia.
    Authors: Martinez-Romero R, Canuelo A, Siles E, Oliver F, Martinez-Lara E
    J Appl Physiol (1985), 2011-12-15;112(5):816-23.  2011-12-15
  31. Poly(ADP-ribose) polymerase 1 (PARP-1) binds to 8-oxoguanine-DNA glycosylase (OGG1).
    Authors: Noren Hooten N, Kompaniez K, Barnes J, Lohani A, Evans M
    J Biol Chem, 2011-11-04;286(52):44679-90.  2011-11-04
  32. Histone H2AX is a critical factor for cellular protection against DNA alkylating agents.
    Authors: Meador JA, Zhao M, Su Y, Narayan G, Geard CR, Balajee AS
    Oncogene, 2008-06-09;27(43):5662-71.  2008-06-09
  33. PARP-1 inhibition prevents oxidative and nitrosative stress-induced endothelial cell death via transactivation of the VEGF receptor 2.
    Authors: Mathews MT, Berk BC
    Arterioscler. Thromb. Vasc. Biol., 2008-01-31;28(4):711-7.  2008-01-31

FAQs

  1. With what species does this kit work?

    • This kit is not species specific. It is suitable for enzymes that will be conserved across all mammals and for tissue homogenates.

  2. What PARP isoform is detected in this kit?

    • This is a universal PARP kit. We expect that all 3 isoforms of PARP will be detected by this kit.

  3. Is the kit capable of measuring PARP hyperactivation (ie. greater than 100% of the PARP positive control)?

    • With this kit, hyperactivation may be possible since it is a cell free system. It is possible that test molecules increase the binding of PARP to the activated DNA or inhibit its dissociation. We are unable to confirm if PARP may become bound to the DNA and trapped within the replication fork or if this would allow for hyperactivation.

  4. What is the ratio of NAD and activated DNA relative to the concentration of enzyme in the final well?

    • The ratio of PARP to DNA is 2:1 and NAD is in excess in the final well.

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