PARP/Apoptosis Chemiluminescent Assay Kit

Catalog #: 4685-096-K
1 Citation 1 Review Datasheet

For the measurment of PARP-1 activity in cell extracts

Catalog #	Availability	Size / Price	Qty
4685-096-K

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Product Details

Citations (1)

FAQs

Reviews (1)

Summary Product Datasheets

PARP/Apoptosis Chemiluminescent Assay Kit Summary

Ideal for measuring the in vitro activity of PARP-1 in cell extracts before and during apoptosis, screening of candidate PARP-1 inhibitors and determination of IC50 values.

Key Benefits

• Chemiluminescent readout
• 96 well format
• Highly sensitive – detects 0.1 mU PARP ~500 cells
• Dynamic range between 0.1 to 10 mU PARP
• Requires 10-100 ng extract for detection
• Assay Time ~3 hrs

Why Use the PARP/Apoptosis Chemiluminescent Assay Kit?

This ELISA based assay detects poly (ADP-ribose) deposited by PARP-1 onto immobilized histones in a 96-well format. An anti-PAR monoclonal detecting antibody followed by addition of a goat anti-rabbit IgG-HRP secondary and a chemiluminescent HRP substrate yields relative light units (RLU) that correlates with PARP-1 activity. During apoptosis PARP-1 is cleaved with a resulting drop in activity. Etoposide is included as a control apoptosis inducer.

Product Specifications

Measure PARP-1 activity in cell extracts before and after apoptosis.
For the screening of candidate PARP-1 inhibitors and determination of IC50 values.

Kit Contents

• 10X Activated DNA
• PARP-HSA, 10 mUnits/µl
• 20 mM NAD
• Anti-PAR monoclonal Antibody
• Goat anti-mouse IgG-HRP
• 10 mM Etoposide
• 20X I-PAR Assay Buffer
• PARP PeroxyGlow A
• PARP PeroxyGlow™ B
• 5X Antibody Diluent
• Histone-Coated White Strip Well Plate, I-PAR

Specifications

Shipping Conditions

The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.

Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Species

Multi-species

Limitations

For research use only. Not for diagnostic use.

Product Datasheets

Product Datasheet

Citation for PARP/Apoptosis Chemiluminescent Assay Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

MUC1-C INTEGRATES CHROMATIN REMODELING AND PARP1 ACTIVITY IN THE DNA DAMAGE RESPONSE OF TRIPLE-NEGATIVE BREAST CANCER CELLS
Authors: M Yamamoto, C Jin, T Hata, Y Yasumizu, Y Zhang, D Hong, T Maeda, M Miyo, M Hiraki, Y Suzuki, K Hinohara, H Rajabi, D Kufe
Cancer Res., 2019-03-01;0(0):. 2019-03-01

FAQs

Is it possible to purchase a smaller version of the PARP/Apoptosis Chemiluminescent Assay Kit?
- R&D Systems does not offer a smaller version of this kit. However, plates are provided in stripwell format, so the user is able to test fewer wells and save the remaining plate strips for later use.
Is it possible to test partial plates when using the PARP/Apoptosis Chemiluminescent Assay Kit?
- The histone-coated plates are provided in stripwell format. To run partial plates, remove excess microplate strips, seal in the provided pouch, and store at 2-8 °C with a dessicant.

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