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PAR/pADPr Alexa Fluor® 350-conjugated Antibody

Catalog #: 4335-MC-100U Datasheet
Catalog # Availability Size / Price Qty
4335-MC-100U-100UG
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PAR/pADPr Alexa Fluor® 350-conjugated Antibody Summary

Specificity
The antibody is specific for PAR polymers 2 to 50 units long, but does not recognize structurally related RNA, DNA, ADP-ribose monomers, NAD, or other nucleic acid monomers.  Detects Poly(ADP-ribose) Polymer in Direct ELISA.
Source
Monoclonal Mouse IgG3 Clone # 10HA
Purification
Protein A or G purified
Immunogen
Purified ADP-ribose polymers between 2 and 50 units long
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration
Sample
ELISA
Optimal dilution of this antibody should be experimentally determined.
 
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
 
Immunoprecipitation
Optimal dilution of this antibody should be experimentally determined.
 
Immunoaffinity Purification
Optimal dilution of this antibody should be experimentally determined.
 
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
 
Intracellular Staining by Flow Cytometry
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PAR/pADPr

PARP [Poly(ADP-ribose) Polymerase], also known as ADPRT and PPOL, is a 118-kDa enzyme that uses NAD as a substrate to catalyze the covalent transfer of ADP-ribose to a variety of nuclear protein acceptors. ADP ribosyltransferase is required for cellular repair, and PARP expression is induced by single-strand breaks in DNA. PARP is proteolytically cleaved by Caspase-3 into two fragments of 89- and 24-kDa in one of the hallmark events of apoptosis.

Long Name
Poly [ADP-ribose] Polymer
Alternate Names
pADPr; PAR; Poly(ADP-ribose)

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