PA Janelia Fluor® 549, NHS ester
Chemical Name: 2,5-Dioxo-1-pyrrolidinyl 3',6'-di-1-azetidinyl-2-diazo-2,3-dihydro-3-oxospiro[1H-indene-1,9'-[9H]xanthene]-6-carboxylate
Purity: ≥90%
Biological Activity
Key information: PA Janelia Fluor® 549, NHS ester is a yellow photoactivatable fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for live and fixed cell imaging.Application: Suitable for single molecule tracking and super resolution microscopy (SRM) in live cells, specifically sptPALM (live cells) and PALM (fixed cells) techniques.
Properties and Photophysical Data: Non-fluorescent until activated at 405 nm. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Can be multiplexed with PA Janelia Fluor® 646, NHS ester (Cat. No. 6150) to perform two-color sptPALM in live cells with activation at 405 nm. Photochemical quantum yield of uncaging = 2.2%, with improved yield upon protein conjugation. Excitation and emission maxima (λ) are 551 - 553 nm and 570 - 573 nm, respectively.
Please see the protocol for further information on protein/antibody labeling and conjugation.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Janelia Fluor is a registered trademark of Howard Hughes Medical Institute.
This product has been recently renamed. The previous name for this product was PA Janelia Fluor® 549, SE.
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Additional Information
Background References
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Bright photoactivatable fluorophores for single-molecule imaging.
Grimm et al.
Nat.Methods, 2016;13:985 -
Real-time imaging of Huntingtin aggregates diverting target search and gene transcription.
Li et al.
eLife, 2016;5:e17056 -
Phosphorylation of the RNA polymerase II C-terminal domain by TFIIH kinase is not essential for transcription of Saccharomyces cerevisiae genome.
Hong et al.
Proc.Natl.Acad.Sci.USA., 2009;106:14276
Product Datasheets
Citation for PA Janelia Fluor® 549, NHS ester
The citations listed below are publications that use Tocris products. Selected citations for PA Janelia Fluor® 549, NHS ester include:
1 Citation: Showing 1 - 1
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Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable cell dyes
Authors: Genshaft Et al.
Nat Commun 2021;12:4995
FAQs
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