NR12S
Chemical Name: 3-((3-((9-(Diethylamino)-5-oxo-5H-benzo[a]phenoxazin-2-yl)oxy)propyl)(dodecyl)(methyl)ammonio)propane-1-sulfonate
Purity: ≥95%
Biological Activity
Key information: NR12S is a fluorogenic lipid membrane dye that stains the outer leaflet of lipid vesicles and cell membranes. Suitable for live cell imaging.Used for: monitoring cholesterol and lipid order selectively at the outer leaflet of cell membranes.
Application: fluorescence microscopy.
Properties and Photophysical Data: NR12S is a conjugation of the Nile Red fluorophore with a long alkyl chain and a zwitterionic group, allowing strong interactions with the lipid membranes. Excitation and emission maxima (λ) are 554 nm and 627 nm in DMSO, respectively; quantum yield = 0.55.
Optical Data for NR12S
| Emission Color | Orange |
|---|---|
| λabs | 554 nm |
| λem | 627 nm |
| Quantum Yield (φ) | 0.55 |
| Closest Laser line | 532 nm |
| Application | Monitoring of Lipid Dynamics in Living Cells, Fluorescence Microscopy |
| Alternative For | Nile Red |
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets
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