Normal Human IgG Control Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of Human IgG by Western Blot Activated ADSCs trigger the proliferation and invasion of lung cancer cells by regulating MMP2/9 expression and EMT. a The effects of activated ADSCs on lung cancer cell proliferation were evaluated using the CCK-8 assay. Four lung cancer cell lines were cultured in the presence of ADSC-CM or aADSC-CM, and the optical density at 450 nm was analysed. The cancer cells cultured alone in normal growth medium were used as negative controls. Data from three separate experiments are shown. b The number of lung cancer cells that migrated through 8-μm Transwell membrane pores was counted to determine the changes in the invasive capabilities in response to CM from epidural ADSCs or aADSCs. c MMP2/9, E-cadherin and vimentin expression levels in four lung cancer cell lines treated with ADSC-CM or aADSC-CM were examined by western blotting. Lung cancer cells cultured in an untreated medium served as controls. d Lung cancer cells were treated with neutralizing antibodies against IL-6, isotype controls and aADSC-CM. Lung cancer cell invasion was analysed using a Transwell assay. Lung cancer cells cultured with untreated medium served as negative controls. e Lung cancer cells were treated with neutralizing antibodies against IL-6, isotype controls and aADSC-CM. MMP2/9, E-cadherin and vimentin expression levels in four lung cancer cell lines were analysed using western blotting. Lung cancer cells cultured with untreated medium served as negative controls. ***P < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31196220), licensed under a CC-BY license. Not internally tested by R&D Systems.
Preparation and Storage
♦ 6 months from date of receipt at 2 to 8 °C
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Product Datasheets
Product Specific Notices
Contains sodium azide. Sodium azide may react with lead and copper plumbing to form explosive metal azides. Flush with large amounts of water during disposal.Citations for Normal Human IgG Control
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Inhibition of shedding of low-density lipoprotein receptor-related protein 1 reverses cartilage matrix degradation in osteoarthritis
Authors: K Yamamoto, S Santamaria, KA Botkjaer, J Dudhia, L Troeberg, Y Itoh, G Murphy, H Nagase
Arthritis & rheumatology (Hoboken, N.J.), 2017-04-28;0(0):.
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Involvement of CD40-CD40L and ICOS-ICOSL in the development of chronic rhinosinusitis by targeting eosinophils
Authors: Aina Zhou, Chenxi Shi, Yuhui Fan, Yushuang Zheng, Jue Wang, Zhichen Liu et al.
Frontiers in Immunology
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Blood-brain barrier penetration of non-replicating SARS-CoV-2 and S1 Variants of Concern induce neuroinflammation which is accentuated in a mouse model of Alzheimer's disease
Authors: MA Erickson, AF Logsdon, EM Rhea, KM Hansen, SJ Holden, WA Banks, JL Smith, C German, SA Farr, JE Morley, RR Weaver, AJ Hirsch, A Kovac, E Kontsekova, KK Baumann, MA Omer, J Raber
Brain, Behavior, and Immunity, 2023-01-20;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Schwann cells promote the migration and invasion of colorectal cancer cells via the activated NF-kappa B/IL-8 axis in the tumor microenvironment
Authors: Shuhai Chen, Mingyou Chen
Frontiers in Oncology
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Dll4 Inhibition Promotes Graft Retention in Fat Grafting Enriched with Adipose-Derived Stem Cells
Authors: Choong-kun Lee, Bo-Yoon Park, Taehee Jo, Cheol-Heum Park, Ju-Hee Kim, Kyu-Jin Chung et al.
Stem Cells Translational Medicine
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A single-cell atlas of non-haematopoietic cells in human lymph nodes and lymphoma reveals a landscape of stromal remodelling
Authors: Yoshiaki Abe, Mamiko Sakata-Yanagimoto, Manabu Fujisawa, Hiroaki Miyoshi, Yasuhito Suehara, Keiichiro Hattori et al.
Nature Cell Biology
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Inhibition of the VEGF signaling pathway attenuates tumor?associated macrophage activity in liver cancer
Authors: S Okikawa, Y Morine, Y Saito, S Yamada, K Tokuda, H Teraoku, K Miyazaki, S Yamashita, T Ikemoto, S Imura, M Shimada
Oncology reports, 2022-02-16;47(4):.
Species: Human
Sample Types: Whole Cells
Applications: Migration Assay Control -
GluA3 autoantibodies induce alterations in dendritic spine and behavior in mice
Authors: D Scheggia, J Stanic, M Italia, F La Greca, E Zianni, A Benussi, B Borroni, M Di Luca, F Gardoni
Brain, Behavior, and Immunity, 2021-07-09;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo Control -
Dissecting spatial heterogeneity and the immune-evasion mechanism of CTCs by single-cell RNA-seq in hepatocellular carcinoma
Authors: YF Sun, L Wu, SP Liu, MM Jiang, B Hu, KQ Zhou, W Guo, Y Xu, Y Zhong, XR Zhou, ZF Zhang, G Liu, S Liu, YH Shi, Y Ji, M Du, NN Li, GB Li, ZK Zhao, XY Huang, LQ Xu, QC Yu, DH Peng, SJ Qiu, HC Sun, M Dean, XD Wang, WY Chung, AR Dennison, J Zhou, Y Hou, J Fan, XR Yang
Nature Communications, 2021-07-02;12(1):4091.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization Control -
Alternol Sensitizes Renal Carcinoma Cells to TRAIL-Induced Apoptosis
Authors: Yu Ren, Xue Wang, Shuaishuai Huang, Yangkai Xu, Guobin Weng, Rui Yu
Frontiers in Pharmacology
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Synthesis and Biological Evaluation of Novel Isoxazole-Amide Analogues as Anticancer and Antioxidant Agents
Authors: AM Eid, M Hawash, J Amer, A Jarrar, S Qadri, I Alnimer, A Sharaf, R Zalmoot, O Hammoudie, S Hameedi, A Mousa
BioMed Research International, 2021-03-09;2021(0):6633297.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
High-Content Analysis-Based Sensitivity Prediction and Novel Therapeutics Screening for c-Met-Addicted Glioblastoma
Authors: JW Oh, YJ Oh, S Han, NG Her, DH Nam
Cancers, 2021-01-20;13(3):.
Species: Human
Sample Types: Whole Cells
Applications: Isotype Control -
The inhibitory effect of TU-100 on hepatic stellate cell activation in the tumor microenvironment
Authors: Y Wada, K Tokuda, Y Morine, S Okikawa, S Yamashita, T Ikemoto, S Imura, Y Saito, S Yamada, M Shimada
Oncotarget, 2020-12-08;11(49):4593-4604.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
An allosteric peptide inhibitor of HIF-1&alpha regulates hypoxia-induced retinal neovascularization
Authors: A Usui-Ouchi, E Aguilar, S Murinello, M Prins, ML Gantner, PE Wright, RB Berlow, M Friedlande
Proc Natl Acad Sci U S A, 2020-10-26;0(0):.
Species: Mouse
Sample Types: Reference Standard
Applications: Control -
Disruption of the tumour-associated EMP3 enhances erythroid proliferation and causes the MAM-negative phenotype
Authors: N Thornton, V Karamatic, L Tilley, CA Green, CL Tay, RE Griffiths, BK Singleton, F Spring, P Walser, AG Alattar, B Jones, R Laundy, JR Storry, M Möller, L Wall, R Charlewood, CM Westhoff, C Lomas-Fran, V Yahalom, U Feick, A Seltsam, B Mayer, ML Olsson, DJ Anstee
Nat Commun, 2020-07-16;11(1):3569.
Species: Human
Sample Types: Whole Cells
Applications: Isotype Control -
Nanoparticle interactions with immune cells dominate tumor retention and induce T cell-mediated tumor suppression in models of breast cancer
Authors: P Korangath, JD Barnett, A Sharma, ET Henderson, J Stewart, SH Yu, SK Kandala, CT Yang, JS Caserto, M Hedayati, TD Armstrong, E Jaffee, C Gruettner, XC Zhou, W Fu, C Hu, S Sukumar, BW Simons, R Ivkov
Sci Adv, 2020-03-25;6(13):eaay1601.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Adipose Tissue-Derived Mesenchymal Stem Cells Suppress Growth of Huh7 Hepatocellular Carcinoma Cells via Interferon (IFN)-beta -Mediated JAK/STAT1 Pathway in vitro
Authors: Chun Sung Byun, Soonjae Hwang, Sung-Hun Woo, Moon Young Kim, Jin Suk Lee, Jong In Lee et al.
International Journal of Medical Sciences
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High Throughput Traction Force Microscopy for Multicellular Islands on Combinatorial Microarrays
Authors: Ian C. Berg, Gregory H. Underhill
BIO-PROTOCOL
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Citrullinated Inhibitor of DNA Binding 1 Is a Novel Autoantigen in Rheumatoid Arthritis
Authors: Ray A. Ohara, Gautam Edhayan, Stephanie M. Rasmussen, Takeo Isozaki, Henriette A. Remmer, Thomas M. Lanigan et al.
Arthritis & Rheumatology
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Immunoglobulin G responses against falciparum malaria specific antigens are higher in children with homozygous sickle cell trait than those with normal hemoglobin
Authors: GM Bwire, M Majigo, R Makalla, L Nkinda, A Mawazo, M Mizinduko, J Makani
BMC Immunol., 2019-04-27;20(1):12.
Species: Human
Sample Types: Protein
Applications: ELISA Standard -
ITGA2 as a potential nanotherapeutic target for glioblastoma
Authors: P Guo, A Moses-Gard, J Huang, ER Smith, MA Moses
Sci Rep, 2019-04-17;9(1):6195.
Species: Human
Sample Types: Whole Cells
Applications: Isotype Control -
Immunoglobulin‑like transcript 4 and human leukocyte antigen‑G interaction promotes the progression of human colorectal cancer
Authors: Zhaoyang Cai, Lu Wang, Yali Han, Wenwen Gao, Xiaojuan Wei, Rumei Gong et al.
International Journal of Oncology
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C1s Inhibition by BIVV009 (Sutimlimab) Prevents Complement-Enhanced Activation of Autoimmune Human B Cells In Vitro
Authors: Pavel A. Nikitin, Eileen L. Rose, Tony S. Byun, Graham C. Parry, Sandip Panicker
The Journal of Immunology
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SPINK1 promotes cell growth and metastasis of lung adenocarcinoma and acts as a novel prognostic biomarker
Authors: Liyun Xu, Changchang Lu, Yanyan Huang, Jihang Zhou, Xincheng Wang, Chaowu Liu et al.
BMB Reports
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Calpain-mediated tau fragmentation is altered in Alzheimer's disease progression
Authors: HH Chen, P Liu, P Auger, SH Lee, O Adolfsson, L Rey-Bellet, J Lafrance-V, BA Friedman, M Pihlgren, A Muhs, A Pfeifer, J Ernst, G Ayalon, KR Wildsmith, TG Beach, MP van der Br
Sci Rep, 2018-11-13;8(1):16725.
Species: Human
Sample Types: Tissue Homogenates
Applications: Immunoprecipitation -
ADAM17 inhibition enhances platinum efficiency in ovarian cancer
Authors: Nina Hedemann, Christoph Rogmans, Susanne Sebens, Daniela Wesch, Manuel Reichert, Dirk Schmidt-Arras et al.
Oncotarget
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Hypoxia-induced activin A diminishes endothelial cell vasculogenic activity
Authors: S Merfeld-Cl, H Lu, X Wu, KL March, DO Traktuev
J. Cell. Mol. Med., 2017-08-18;0(0):.
Species: Human
Sample Types: Whole Cells
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Comprehensive cross-clade characterization of antibody-mediated recognition, complement-mediated lysis and cell-mediated cytotoxicity of HIV-1 envelope specific antibodies towards the eradication of the HIV-1 reservoir
Authors: S Mujib, J Liu, AKMN Rahman, JA Schwartz, P Bonner, FY Yue, MA Ostrowski
J. Virol., 2017-07-27;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
iPSC-derived mesenchymal stem cells exert SCF-dependent recovery of cigarette smoke-induced apoptosis/proliferation imbalance in airway cells
Authors: Xiang Li, Yuelin Zhang, Yingmin Liang, Yuting Cui, Sze C. Yeung, Mary S.M. Ip et al.
Journal of Cellular and Molecular Medicine
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HCV RNA Activates APCs via TLR7/TLR8 While Virus Selectively Stimulates Macrophages Without Inducing Antiviral Responses
Sci Rep, 2016-07-07;6(0):29447.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Combinatorial microenvironmental regulation of liver progenitor differentiation by Notch ligands, TGF beta, and extracellular matrix
Authors: Kerim B. Kaylan, Viktoriya Ermilova, Ravi Chandra Yada, Gregory H. Underhill
Scientific Reports
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Glycoprotein nonmetastatic melanoma protein B extracellular fragment shows neuroprotective effects and activates the PI3K/Akt and MEK/ERK pathways via the Na+/K+-ATPase.
Authors: Yoko Ono, Kazuhiro Tsuruma, Masafumi Takata, Masamitsu Shimazawa, Hideaki Hara
Scientific Reports, 2016-03-18;0(0):2045-2322.
Species: Mouse
Sample Types: Cell Lysates
Applications: Control -
Active immunization with Tocilizumab mimotopes induces specific immune responses.
Authors: Yang L, Xing R, Li C, Liu Y, Sun L, Liu X, Wang Y
BMC Biotechnol, 2015-06-02;15(0):46.
Species: Bacteria - E. Coli
Sample Types: Virus
Applications: Isotype Control -
Targeting ADAM-17 with an inhibitory monoclonal antibody has antitumour effects in triple-negative breast cancer cells.
Authors: Caiazza F, McGowan P, Mullooly M, Murray A, Synnott N, O'Donovan N, Flanagan L, Tape C, Murphy G, Crown J, Duffy M
Br J Cancer, 2015-05-26;112(12):1895-903.
Species: Human
Sample Types: Whole Cells
Applications: Isotype Control -
Coronary artery endothelial cells and microparticles increase expression of VCAM-1 in myocardial infarction
Authors: Christopher E. Radecke, Alexandra E. Warrick, Gagan D. Singh, Jason H. Rogers, Scott I. Armstrong, Ehrin J. Armstrong
Thrombosis and Haemostasis
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Experimental study of endothelial progenitor cells labeled with superparamagnetic iron oxide in vitro
Authors: MENG-QI WEI, DI-DI WEN, XIAO-YING WANG, YI HUAN, YONG YANG, JIAN XU et al.
Molecular Medicine Reports
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Pericytes contribute to the disruption of the cerebral endothelial barrier via increasing VEGF expression: implications for stroke.
Authors: Bai Y, Zhu X, Chao J, Zhang Y, Qian C, Li P, Liu D, Han B, Zhao L, Zhang J, Buch S, Teng G, Hu G, Yao H
PLoS ONE, 2015-04-17;10(4):e0124362.
Species: Mouse
Sample Types: Whole Organism
Applications: Control -
Hypoxia down-regulates expression of secretory leukocyte protease inhibitor in bronchial epithelial cells via TGF-beta1.
Authors: Pahlman L, Jogi A, Gram M, Mori M, Egesten A
BMC Pulm Med, 2015-03-07;15(0):19.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
B7-H1-expressing antigen-presenting cells mediate polarization of protumorigenic Th22 subsets.
Authors: Kuang D, Xiao X, Zhao Q, Chen M, Li X, Liu R, Wei Y, Ouyang F, Chen D, Wu Y, Lao X, Deng H, Zheng L
J Clin Invest, 2014-09-17;124(10):4657-67.
Species: Human
Sample Types: Whole Cells
Applications: Control -
A novel murine cytomegalovirus vaccine vector protects against Mycobacterium tuberculosis.
Authors: Beverley P, Ruzsics Z, Hey A, Hutchings C, Boos S, Bolinger B, Marchi E, O'Hara G, Klenerman P, Koszinowski U, Tchilian E
J Immunol, 2014-07-28;193(5):2306-16.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
RepSox slows decay of CD34+ acute myeloid leukemia cells and decreases T cell immunoglobulin mucin-3 expression.
Authors: Jajosky A, Coad J, Vos J, Martin K, Senft J, Wenger S, Gibson L
Stem Cells Transl Med, 2014-05-22;3(7):836-48.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Induction and activation of human Th17 by targeting antigens to dendritic cells via dectin-1.
Authors: Duluc D, Joo H, Ni L, Yin W, Upchurch K, Li D, Xue Y, Klucar P, Zurawski S, Zurawski G, Oh S
J Immunol, 2014-05-16;192(12):5776-88.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Pathogenic strains of Acanthamoeba are recognized by TLR4 and initiated inflammatory responses in the cornea.
Authors: Alizadeh H, Tripathi T, Abdi M, Smith A
PLoS ONE, 2014-03-14;9(3):e92375.
Species: Human
Sample Types: Whole Cells
Applications: Isotype Control -
The aryl hydrocarbon receptor is functionally upregulated early in the course ofvhuman T-cell activation.
Authors: Prigent L, Robineau M, Jouneau S, Morzadec C, Louarn L, Vernhet L, Fardel O, Sparfel L
Eur J Immunol, 2014-02-18;44(5):1330-40.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Generation of neuronal progenitor cells in response to tumors in the human brain.
Authors: Macas J, Ku M, Nern C, Xu Y, Buhler H, Remke M, Synowitz M, Franz K, Seifert V, Plate K, Kettenmann H, Glass R, Momma S
Stem Cells, 2014-01-01;32(1):244-57.
Species: Human
Sample Types: Whole Cells
Applications: Control -
Angiogenesis stimulated by human kallikrein-related peptidase 12 acting via a platelet-derived growth factor B-dependent paracrine pathway.
Authors: Kryza T, Achard C, Parent C, Marchand-Adam S, Guillon-Munos A, Iochmann S, Korkmaz B, Respaud R, Courty Y, Heuze-Vourc'h N
FASEB J, 2013-11-13;28(2):740-51.
Species: Human
Sample Types: Protein, Whole Cells
Applications: IHC, Western Blot -
Activation of endothelial TLR2 by bacterial lipoprotein upregulates proteins specific for the neutrophil response
Authors: Kevin Wilhelmsen, Kailin R Mesa, Arun Prakash, Fengyun Xu, Judith Hellman
Innate Immunity
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Anti-Tumour Effects of a Specific Anti-ADAM17 Antibody in an Ovarian Cancer Model In Vivo.
Authors: Richards FM, Tape CJ, Jodrell DI, Murphy G
PLoS ONE, 2012-07-11;7(7):e40597.
Species: Human
Sample Types: Whole Cells
Applications: Control -
ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes.
Authors: Wilhelmsen K, Mesa K, Lucero J, Xu F, Hellman J
J Biol Chem, 2012-06-15;287(32):26478-94.
Species: Human
Sample Types: Whole Cells
Applications: Blocking -
Sjogren's syndrome pathological neovascularization is regulated by VEGF-A-stimulated TACE-dependent crosstalk between VEGFR2 and NF-kappaB.
Authors: Sisto M, Lisi S, Lofrumento DD, D'Amore M, Frassanito MA, Ribatti D
Genes Immun., 2012-04-19;13(5):411-20.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Tumor cell marker PVRL4 (nectin 4) is an epithelial cell receptor for measles virus.
Authors: Noyce RS, Bondre DG, Ha MN
PLoS Pathog., 2011-08-25;7(8):e1002240.
Species: Hamster, Human
Sample Types: Whole Cells
Applications: Fc Blocking -
Inhibition of Cell Proliferation by an Anti-EGFR Aptamer.
Authors: Li N, Nguyen HH, Byrom M, Ellington AD
PLoS ONE, 2011-06-08;6(6):e20299.
Species: Human
Sample Types: RNA
Applications: Control -
HER2 phosphorylation is maintained by a PKB negative feedback loop in response to anti-HER2 herceptin in breast cancer.
Authors: Gijsen M, King P, Perera T, Parker PJ, Harris AL, Larijani B, Kong A
PLoS Biol., 2010-12-21;8(12):e1000563.
Species: Human
Sample Types: Whole Cells
Applications: Control -
Suppression of TNF-alpha and IL-1 signaling identifies a mechanism of homeostatic regulation of macrophages by IL-27.
Authors: Kalliolias GD, Gordon RA, Ivashkiv LB
J. Immunol., 2010-10-22;185(11):7047-56.
Species: Human
Sample Types: Whole Cells
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TSU68 prevents liver metastasis of colon cancer xenografts by modulating the premetastatic niche.
Authors: Yamamoto M, Kikuchi H, Ohta M, Kawabata T, Hiramatsu Y, Kondo K, Baba M, Kamiya K, Tanaka T, Kitagawa M, Konno H
Cancer Res., 2008-12-01;68(23):9754-62.
Species: Mouse
Sample Types: In Vivo
Applications: Control -
Spatial patterning of liver progenitor cell differentiation mediated by cellular contractility and Notch signaling
Authors: KB Kaylan, IC Berg, MJ Biehl, A Brougham-C, I Jain, SM Jamil, LH Sargeant, NJ Cornell, LT Raetzman, GH Underhill
Elife, 2018-12-27;7(0):.
FAQs
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What subtype of IgG does Normal Human IgG Control (Catalog # 1-001-A) contain?
Normal Human IgG Control (Catalog # 1-001-A) is produced by purification of IgG from Normal human plasma. It may contain more than one subtype of IgG.
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